Alternative Ion-Pairing Modifiers Should Be Investigated in Low-Input and Single-Cell Proteomics

A recent study demonstrated a substantial increase in the peptide signal and corresponding proteome coverage when employing 0.5% acetic acid (AA) as the ion pairing modifier in place of the 0.1% formic acid traditionally used in shotgun proteomics. Given the strictly limited material and counterintu...

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Bibliographic Details
Main Author:	Colten D. Eberhard (17729446) (author)
Other Authors:	Cameron Braswell (22674649) (author), Benjamin C. Orsburn (9306361) (author)
Published:	2025
Subjects:	Biophysics Biochemistry Medicine Biological Sciences not elsewhere classified Chemical Sciences not elsewhere classified subnanogram proteome dilutions strictly limited material reduce ramp times recent study demonstrated increased proteome coverage corresponding proteome coverage 30 min analysis ion pairing modifiers ion pairing modifier pairing modifiers alternative ion alternative modifier vendor raw shotgun proteomics scans across processed data peptide groups lower concentrations emerging field digest standards counterintuitive observations cell proteomics approximately 13 additives warrant acetic acid 7 ×
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Alternative Ion-Pairing Modifiers Should Be Investigated in Low-Input and Single-Cell Proteomics

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