Alternative Ion-Pairing Modifiers Should Be Investigated in Low-Input and Single-Cell Proteomics

Alternative Ion-Pairing Modifiers Should Be Investigated in Low-Input and Single-Cell Proteomics

A recent study demonstrated a substantial increase in the peptide signal and corresponding proteome coverage when employing 0.5% acetic acid (AA) as the ion pairing modifier in place of the 0.1% formic acid traditionally used in shotgun proteomics. Given the strictly limited material and counterintu...

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Detalhes bibliográficos
Autor principal: Colten D. Eberhard (17729446) (author)
Outros Autores: Cameron Braswell (22674649) (author), Benjamin C. Orsburn (9306361) (author)
Publicado em: 2025
Assuntos:
Biophysics
Biochemistry
Medicine
Biological Sciences not elsewhere classified
Chemical Sciences not elsewhere classified
subnanogram proteome dilutions
strictly limited material
reduce ramp times
recent study demonstrated
increased proteome coverage
corresponding proteome coverage
30 min analysis
ion pairing modifiers
ion pairing modifier
pairing modifiers
alternative ion
alternative modifier
vendor raw
shotgun proteomics
scans across
processed data
peptide groups
lower concentrations
emerging field
digest standards
counterintuitive observations
cell proteomics
approximately 13
additives warrant
acetic acid
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