Summary of Substituted Cysteine Modification and Anesthetic Protection Studies in β3 Subunits.

<p>See <b><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0336606#pone.0336606.g003" target="_blank">Fig 3</a></b> legend for detailed description. Protection was inferred when an anesthetic significantly reduced modification ra...

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Váldodahkki:	Kieran Bhave (9234643) (author)
Eará dahkkit:	Stuart A. Forman (649631) (author)
Almmustuhtton:	2025
Fáttát:	Biophysics Biochemistry Cell Biology Genetics Molecular Biology Biotechnology Evolutionary Biology Cancer Infectious Diseases Computational Biology Chemical Sciences not elsewhere classified general anesthesia induced 8 residues located mpab binds selectively substituted cysteine modification pcmbs modification effects approaches also reveal acid level analysis homologous sites formed five homologous sites performed using etomidate synaptic αβγ gaba produced functional gaba outer transmembrane inter etomidate binds selectively control scamp studies 5 point mutations 3 &# 947 1 &# 946 &# 945 ;+ bnoh binds selectively xlink "> incorporation anesthetic photolabel ptfd synaptic gaba xlink "> functional analysis &# 947 &# 946 &# 945 photolabel incorporation anesthetic photolabel bind selectively etomidate sites pcmbs ). cysteine mutations approaches based subunit sites interfacial sites used voltage retained sensitivity positive modulation pentameric ligand inhibitory hetero electron micrography clamp electrophysiology bound ptfd bnoh modulation based largely </ sub
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Čoahkkáigeassu:	<p>See <b><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0336606#pone.0336606.g003" target="_blank">Fig 3</a></b> legend for detailed description. Protection was inferred when an anesthetic significantly reduced modification ratios (p < 0.05) relative to control. Below each bar graph is a diagram showing the interfaces where the mutation is located. <b>Panel A:</b> β3L231C (β–) was protected by both R-mTFD-MPAB and pTFD-di-iPr-BnOH. <b>Panel B:</b> β3M286C (β+) was protected by etomidate. ETO = etomidate; MPAB = R-mTFD-MPAB; iPr-BnOH = pTFD-di-iPr-BnOH; pCMBS = para-chloromercuribenzenesulfonate.</p>

Summary of Substituted Cysteine Modification and Anesthetic Protection Studies in β3 Subunits.

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