Summary of Substituted Cysteine Modification and Anesthetic Protection Studies in β3 Subunits.

Summary of Substituted Cysteine Modification and Anesthetic Protection Studies in β3 Subunits.

<p>See <b><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0336606#pone.0336606.g003" target="_blank">Fig 3</a></b> legend for detailed description. Protection was inferred when an anesthetic significantly reduced modification ra...

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Manylion Llyfryddiaeth
Prif Awdur:	Kieran Bhave (9234643) (author)
Awduron Eraill:	Stuart A. Forman (649631) (author)
Cyhoeddwyd:	2025
Pynciau:	Biophysics Biochemistry Cell Biology Genetics Molecular Biology Biotechnology Evolutionary Biology Cancer Infectious Diseases Computational Biology Chemical Sciences not elsewhere classified general anesthesia induced 8 residues located mpab binds selectively substituted cysteine modification pcmbs modification effects approaches also reveal acid level analysis homologous sites formed five homologous sites performed using etomidate synaptic αβγ gaba produced functional gaba outer transmembrane inter etomidate binds selectively control scamp studies 5 point mutations 3 &# 947 1 &# 946 &# 945 ;+ bnoh binds selectively xlink "> incorporation anesthetic photolabel ptfd synaptic gaba xlink "> functional analysis &# 947 &# 946 &# 945 photolabel incorporation anesthetic photolabel bind selectively etomidate sites pcmbs ). cysteine mutations approaches based subunit sites interfacial sites used voltage retained sensitivity positive modulation pentameric ligand inhibitory hetero electron micrography clamp electrophysiology bound ptfd bnoh modulation based largely </ sub
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Testing Dex alone and Dex with different doses of Midazolam in male rats that were 3-4 months old. The stimulus was applied at different time points in an experiment. Responses were tabulated and are presented numerically in the table. Statistical difference is calculated with Fisher’s exact test. Data were obtained from 8 male rats.
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Testing Dex alone or Dex supplemented with midazolam in 3–4-month-old (left two columns) and 11–12-month-old female rats (right two columns). A noxious stimulus was applied every 15 minutes during an experiment. Responses were tabulated and statistical difference is calculated with Fisher’s exact test. Data were obtained from the same group of 8 female rats.
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Testing Dex alone or Dex supplemented with Mg<sup>2+</sup> in old female (20-21 months) and male rats (18-19 months). The rats were exposed to Dex with Mg<sup>2+</sup> as indicated. A noxious stimulus was applied at different time points in an experiment. Responses were tabulated and are presented numerically in the table. The data is shown for illustrative purposes only.
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Testing Dex supplemented with Mg<sup>2+</sup> in 11-12 month old male rats. A noxious stimulus was applied at the times indicated. Responses were tabulated and are presented numerically in the table. Statistical difference is calculated with Fisher’s exact test. This same group of rats gained a significant amount of weight from 3–4-month-old to 11-12-month-old, p = 0.0014.
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Testing Dex alone or Dex supplemented with Mg<sup>2+</sup> or Dex supplemented with both Mg<sup>2+</sup> and midazolam in 3–4-month-old male rats. A noxious stimulus was applied at different time points in an experiment. Responses were tabulated and are presented numerically in the table. Statistical difference is calculated with Fisher’s exact test. Data were obtained from 8 male rats. Note that Dex with Midazolam at 0.3 mg/kg bolus and 0.1 mg/kg/hr was not sufficient to suppress the tail clamp tests in these rats.
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Testing Dex alone or Dex supplemented with Mg<sup>2+</sup> in female rats that were either 3-4 months old (left 2 columns) or 11-12 months old (right 2 columns). A noxious stimulus was applied at different time points in each experiment, as indicated. Responses were tabulated and are presented numerically in the table. Statistical difference is calculated with Fisher’s exact test. Repeating the tail clamp experiment in the two young rats that responded to the stimulus with same dose of Dex but an increased level of Mg<sup>2+</sup> (100 mg/kg bolus and 50 mg/kg/hr infusion), completely suppressed all response to the stimulus. None of the older rats responded to the tail clamp at the lower dose of Mg<sup>2+</sup> with Dex.
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A representative illustration of an older female rat (11-12 months) that underwent abdominal surgery that received Dex supplemented with Mg<sup>2+</sup> with no other agents.
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Distribution of binding preferences.
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