Phage susceptibility of FM2.5 complemented with SLCT-H4 or SLCT-H8.

<p>Spot tests on FM2.5 strains expressing SLCT-H4 or SLCT-H8. Serial 10-fold dilutions of the indicated phages (undiluted titers >10⁸ PFU/mL) were spotted onto bacterial lawns. For ΦMMP04, the observed lysis zone on the SLCT-H4-complemented strain is considered to result from a lysis from w...

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Bibliographic Details
Main Author:	Alexia L.M. Royer (22693792) (author)
Other Authors:	Émeline Dion (22693795) (author), Andrew A. Umansky (22693798) (author), Mariano Avino (243049) (author), Olga Soutourina (248942) (author), Louis-Charles Fortier (225877) (author)
Published:	2025
Subjects:	Biochemistry Microbiology Molecular Biology Biotechnology Ecology Immunology Cancer Infectious Diseases Virology Biological Sciences not elsewhere classified remains poorly understood provide new insights div >< p creating chimeric constructs expressing modified isoforms phages adsorbed efficiently layer protein contribute lmw d2 domain govern phage binding clostridioides difficile </ mediated phage recognition general phage receptor phage infection cycle layer protein slpa layer protein receptor binding difficile </ phage recognition different isoforms successful infection slpa </ several phages seven phages productive infection precise recognition phage therapy phage specificity phage cocktails structural features structural determinants slpa required results demonstrate r20291 δ promising alternative previously identified molecular determinants host receptor hmw fragments highlighting differences future design fundamental importance frequently required dependent manner delayed adsorption critical step broad spectrum always predictive always essential also able
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Phage susceptibility of FM2.5 complemented with SLCT-H4 or SLCT-H8.

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