<i>N. brasiliensis</i> infection is comparable between BALB/c and IL-4Rα<sup>−/lox</sup> mice.

<p>Five mice per group were infected with 750 <i>N. brasiliensis</i> L3 larvae. Faeces were collected from day 5 to 10 post infection (PI) and egg production was calculated using the modified McMaster technique (A). At days 7 and 10 PI the worm burden in the small intestine was ass...

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Main Author: Saskia Schmidt (109610) (author)
Other Authors: J. Claire Hoving (7407029) (author), William G. C. Horsnell (12696689) (author), Helen Mearns (109612) (author), Antony J. Cutler (9024605) (author), Tiroyaone Masego H. Bogale (22681164) (author), Frank Brombacher (52142) (author)
Published: 2025
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Summary:<p>Five mice per group were infected with 750 <i>N. brasiliensis</i> L3 larvae. Faeces were collected from day 5 to 10 post infection (PI) and egg production was calculated using the modified McMaster technique (A). At days 7 and 10 PI the worm burden in the small intestine was assessed (B). Intestinal goblet cell hyperplasia was assessed by determining the total number of PAS-positive goblet cells per 5 villi in histological sections of the small intestine at day 7 and 10 PI (C). Total IgE production in the serum was measured by ELISA at day 7 and 10 PI (D). Comparison of the response of infected BALB/c and IL-4Rα<sup>−/lox</sup> mice to acetylcholine is also shown for day 7 p.i. The data represents one (A-D) and two (E) independent experiment with n = 5 per group and mean values + SEM. ND, not detected. Unpaired two-tailed Student t test, ns = not significant.</p> <p>(TIF)</p>