Antibody verification.
<p>(A) Overexpression verification. HEK293T cells were transfected with CFAP77-, CCDC105-, or TEX43-tagged plasmids; both tag-antibodies and their antibodies recognized a specific band at the same molecular size. (B) Knockout validation. CFAP77 was absent in sperm samples of <i>Cfap77<...
সংরক্ষণ করুন:
| প্রধান লেখক: | |
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| অন্যান্য লেখক: | , , , , , |
| প্রকাশিত: |
2025
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| বিষয়গুলি: | |
| ট্যাগগুলো: |
ট্যাগ যুক্ত করুন
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| সংক্ষিপ্ত: | <p>(A) Overexpression verification. HEK293T cells were transfected with CFAP77-, CCDC105-, or TEX43-tagged plasmids; both tag-antibodies and their antibodies recognized a specific band at the same molecular size. (B) Knockout validation. CFAP77 was absent in sperm samples of <i>Cfap77</i>-KO mice. (C) siRNAs targeting <i>Ccdc105</i> and <i>Tex43</i> reduced their signals in HEK293T cells transfected with CCDC105- or TEX43-tagged plasmids, respectively. The siRNA sequences were listed as follows. NC siRNA: UUCUCCGAACGUGUCACGUTTACGUGACACGUUCGGAGAATT; siRNA targeting <i>Ccdc105</i>: CGUGUGCUAAGGCCUUGUUTTAACAAGGCCUUAGCACACGTT; siRNA targeting <i>Tex43</i>: GGUGGGACGAUAUUCACUUTTAAGUGAAUAUCGUCCCACCTT. Raw blot images can be found in S1 Raw Images.</p> <p>(TIF)</p> |
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