Automated Enrichment and Coextraction Pipeline for Efficient Analysis of Urinary Extracellular Vesicle Protein and RNA

Extracellular vesicle (EV) cargoes have great potential as promising biomarkers for disease diagnosis. However, traditional methods for EV enrichment and cargo extraction are inefficient, limiting the accessibility of valuable biological information. Here, we present a bead-based pipeline designed t...

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Bibliographic Details
Main Author:	Yufeng Liu (307498) (author)
Other Authors:	Guiyuan Zhang (13994877) (author), Yunwu Li (2736502) (author), Dong Wei (446858) (author), Hao Zhang (15339) (author), Anton Iliuk (247495) (author), Zhuoying Xie (1716043) (author), Yanhong Gu (565582) (author), Zhongze Gu (313751) (author), Yefei Zhu (440677) (author), Ying Zhang (40767) (author)
Published:	2025
Subjects:	Biophysics Biochemistry Cell Biology Genetics Molecular Biology Pharmacology Biotechnology Developmental Biology Cancer Hematology Space Science Biological Sciences not elsewhere classified Chemical Sciences not elsewhere classified Physical Sciences not elsewhere classified Information Systems not elsewhere classified valuable biological information efficiently enrich evs approximately 2 days streamlined pipeline provides new pipeline achieved based pipeline designed advancing noninvasive diagnostics rna markers achieving rna extracellular vesicle holds great potential great potential coextraction pipeline noninvasive diagnosis rna yield rna extraction traditional methods simultaneously extracting robust tool protein extraction prostate cancer promising biomarkers pca ), fold improvement disease diagnosis clinical settings cargo extraction automated enrichment acetone precipitation 1 h
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Automated Enrichment and Coextraction Pipeline for Efficient Analysis of Urinary Extracellular Vesicle Protein and RNA

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