Filamin A KO and S2152A KI reduce KSHV de novo infection and cell-contact mediated viral infection.
<p>A. Filamin WT, S2152A KI or KO HEK293-mCherry cells were infected with purified iSLK.Bac16 or iSLK.STOP45 viral stocks (MOI = 10) for 24 h. The cells were harvested and analyzed by FACS flow cytometer. The representative images and the percentages of KSHV-infected HEK293-mCherry cells were...
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2025
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| Резюме: | <p>A. Filamin WT, S2152A KI or KO HEK293-mCherry cells were infected with purified iSLK.Bac16 or iSLK.STOP45 viral stocks (MOI = 10) for 24 h. The cells were harvested and analyzed by FACS flow cytometer. The representative images and the percentages of KSHV-infected HEK293-mCherry cells were calculated and shown in three independent experiments. B. After lytic induction for 72 h, iSLK.Bac16 or iSLK.STOP45 cells were collected and washed twice, and directly added to the monolayer of Filamin WT, S2152A KI or KO HEK293-mCherry cells and co-cultured for additional 24 h. Total cells were harvested and analyzed by FACS flow cytometer. The representative images of mCherry-positive cells are shown and the percentages of KSHV-infected HEK293-mCherry cells were calculated in three independent experiments and shown. **, p < 0.01; ***, p < 0.001; ****, p < 0.0001, t test.</p> |
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