Filamin A KO and S2152A KI reduce KSHV de novo infection and cell-contact mediated viral infection.
<p>A. Filamin WT, S2152A KI or KO HEK293-mCherry cells were infected with purified iSLK.Bac16 or iSLK.STOP45 viral stocks (MOI = 10) for 24 h. The cells were harvested and analyzed by FACS flow cytometer. The representative images and the percentages of KSHV-infected HEK293-mCherry cells were...
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| _version_ | 1849927641312788480 |
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| author | Xiaojuan Li (351842) |
| author2 | Li Quan (177024) Rihong Zhou (22676690) Xiangpeng Liu (17898881) Ronit Sarid (186795) Ersheng Kuang (129028) |
| author2_role | author author author author author |
| author_facet | Xiaojuan Li (351842) Li Quan (177024) Rihong Zhou (22676690) Xiangpeng Liu (17898881) Ronit Sarid (186795) Ersheng Kuang (129028) |
| author_role | author |
| dc.creator.none.fl_str_mv | Xiaojuan Li (351842) Li Quan (177024) Rihong Zhou (22676690) Xiangpeng Liu (17898881) Ronit Sarid (186795) Ersheng Kuang (129028) |
| dc.date.none.fl_str_mv | 2025-11-24T18:34:27Z |
| dc.identifier.none.fl_str_mv | 10.1371/journal.ppat.1013737.g006 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/figure/Filamin_A_KO_and_S2152A_KI_reduce_KSHV_de_novo_infection_and_cell-contact_mediated_viral_infection_/30697709 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Microbiology Cell Biology Molecular Biology Biotechnology Immunology Developmental Biology Cancer Infectious Diseases Virology s2152a knockin abolish primary effusion lymphoma multicentric castleman disease kaposi &# 8217 mediated viral infection infected adherent cells differentiated b cells sequential cell detachment mediated kshv transmission kshv lytic replication adherent cells lytic replication viral transmission kshv transmission cell detachment b lymphocytes lytic cycle cell transmission taken together results demonstrated radically support natural donors key role key regulator etiological agent dependent manner cell contact cell adhesion associated herpesvirus |
| dc.title.none.fl_str_mv | Filamin A KO and S2152A KI reduce KSHV de novo infection and cell-contact mediated viral infection. |
| dc.type.none.fl_str_mv | Image Figure info:eu-repo/semantics/publishedVersion image |
| description | <p>A. Filamin WT, S2152A KI or KO HEK293-mCherry cells were infected with purified iSLK.Bac16 or iSLK.STOP45 viral stocks (MOI = 10) for 24 h. The cells were harvested and analyzed by FACS flow cytometer. The representative images and the percentages of KSHV-infected HEK293-mCherry cells were calculated and shown in three independent experiments. B. After lytic induction for 72 h, iSLK.Bac16 or iSLK.STOP45 cells were collected and washed twice, and directly added to the monolayer of Filamin WT, S2152A KI or KO HEK293-mCherry cells and co-cultured for additional 24 h. Total cells were harvested and analyzed by FACS flow cytometer. The representative images of mCherry-positive cells are shown and the percentages of KSHV-infected HEK293-mCherry cells were calculated in three independent experiments and shown. **, p < 0.01; ***, p < 0.001; ****, p < 0.0001, t test.</p> |
| eu_rights_str_mv | openAccess |
| id | Manara_88068b401c9bbc58754f179c449cf327 |
| identifier_str_mv | 10.1371/journal.ppat.1013737.g006 |
| network_acronym_str | Manara |
| network_name_str | ManaraRepo |
| oai_identifier_str | oai:figshare.com:article/30697709 |
| publishDate | 2025 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Filamin A KO and S2152A KI reduce KSHV de novo infection and cell-contact mediated viral infection.Xiaojuan Li (351842)Li Quan (177024)Rihong Zhou (22676690)Xiangpeng Liu (17898881)Ronit Sarid (186795)Ersheng Kuang (129028)MicrobiologyCell BiologyMolecular BiologyBiotechnologyImmunologyDevelopmental BiologyCancerInfectious DiseasesVirologys2152a knockin abolishprimary effusion lymphomamulticentric castleman diseasekaposi &# 8217mediated viral infectioninfected adherent cellsdifferentiated b cellssequential cell detachmentmediated kshv transmissionkshv lytic replicationadherent cellslytic replicationviral transmissionkshv transmissioncell detachmentb lymphocyteslytic cyclecell transmissiontaken togetherresults demonstratedradically supportnatural donorskey rolekey regulatoretiological agentdependent mannercell contactcell adhesionassociated herpesvirus<p>A. Filamin WT, S2152A KI or KO HEK293-mCherry cells were infected with purified iSLK.Bac16 or iSLK.STOP45 viral stocks (MOI = 10) for 24 h. The cells were harvested and analyzed by FACS flow cytometer. The representative images and the percentages of KSHV-infected HEK293-mCherry cells were calculated and shown in three independent experiments. B. After lytic induction for 72 h, iSLK.Bac16 or iSLK.STOP45 cells were collected and washed twice, and directly added to the monolayer of Filamin WT, S2152A KI or KO HEK293-mCherry cells and co-cultured for additional 24 h. Total cells were harvested and analyzed by FACS flow cytometer. The representative images of mCherry-positive cells are shown and the percentages of KSHV-infected HEK293-mCherry cells were calculated in three independent experiments and shown. **, p < 0.01; ***, p < 0.001; ****, p < 0.0001, t test.</p>2025-11-24T18:34:27ZImageFigureinfo:eu-repo/semantics/publishedVersionimage10.1371/journal.ppat.1013737.g006https://figshare.com/articles/figure/Filamin_A_KO_and_S2152A_KI_reduce_KSHV_de_novo_infection_and_cell-contact_mediated_viral_infection_/30697709CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/306977092025-11-24T18:34:27Z |
| spellingShingle | Filamin A KO and S2152A KI reduce KSHV de novo infection and cell-contact mediated viral infection. Xiaojuan Li (351842) Microbiology Cell Biology Molecular Biology Biotechnology Immunology Developmental Biology Cancer Infectious Diseases Virology s2152a knockin abolish primary effusion lymphoma multicentric castleman disease kaposi &# 8217 mediated viral infection infected adherent cells differentiated b cells sequential cell detachment mediated kshv transmission kshv lytic replication adherent cells lytic replication viral transmission kshv transmission cell detachment b lymphocytes lytic cycle cell transmission taken together results demonstrated radically support natural donors key role key regulator etiological agent dependent manner cell contact cell adhesion associated herpesvirus |
| status_str | publishedVersion |
| title | Filamin A KO and S2152A KI reduce KSHV de novo infection and cell-contact mediated viral infection. |
| title_full | Filamin A KO and S2152A KI reduce KSHV de novo infection and cell-contact mediated viral infection. |
| title_fullStr | Filamin A KO and S2152A KI reduce KSHV de novo infection and cell-contact mediated viral infection. |
| title_full_unstemmed | Filamin A KO and S2152A KI reduce KSHV de novo infection and cell-contact mediated viral infection. |
| title_short | Filamin A KO and S2152A KI reduce KSHV de novo infection and cell-contact mediated viral infection. |
| title_sort | Filamin A KO and S2152A KI reduce KSHV de novo infection and cell-contact mediated viral infection. |
| topic | Microbiology Cell Biology Molecular Biology Biotechnology Immunology Developmental Biology Cancer Infectious Diseases Virology s2152a knockin abolish primary effusion lymphoma multicentric castleman disease kaposi &# 8217 mediated viral infection infected adherent cells differentiated b cells sequential cell detachment mediated kshv transmission kshv lytic replication adherent cells lytic replication viral transmission kshv transmission cell detachment b lymphocytes lytic cycle cell transmission taken together results demonstrated radically support natural donors key role key regulator etiological agent dependent manner cell contact cell adhesion associated herpesvirus |