SYTOX Green fluorescence measurements for membrane permeability assessment.

SYTOX Green fluorescence measurements for membrane permeability assessment.

<p>SYTOX Green fluorescence measurements for membrane permeability assessment.</p>

-д хадгалсан:

Номзүйн дэлгэрэнгүй
Үндсэн зохиолч:	Jianhua Liao (1393228) (author)
Бусад зохиолчид:	Jun Cheng (194158) (author), Baoqing Liu (1508821) (author), Yuzhi Shao (22683349) (author), Chunyan Meng (5993228) (author)
Хэвлэсэн:	2025
Нөхцлүүд:	Medicine Microbiology Cell Biology Biotechnology Evolutionary Biology Ecology Science Policy Infectious Diseases Virology Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified therapeutic strategies aimed novel therapeutic approaches murine infection model div >< p defense mechanisms employed conserved gene encoding compromising membrane stability affect biofilm formation staphylococcus aureus </ reduced bacterial burden improved host survival maintaining mrsa ’ oxidative stress resistance yqhg </ withstand host reduced motility oxidative stress increasing resistance stress adaptation potential target periplasmic protein mrsa virulence mrsa pathogenesis key determinant infected organs increased sensitivity imposed stresses growing prevalence findings highlight existing antibiotics critical need attenuates virulence
Шошгууд:	Шошго нэмэх Шошго байхгүй, Энэхүү баримтыг шошголох эхний хүн болох!

Ижил төстэй зүйлс

Primers Used in This Study.
-н: Jianhua Liao (1393228)
Хэвлэсэн: (2025)

Bacterial Strains and Plasmids Used in This Study.
-н: Jianhua Liao (1393228)
Хэвлэсэн: (2025)

Oxidative stress resistance of the <i>yqhG</i> mutant.
-н: Jianhua Liao (1393228)
Хэвлэсэн: (2025)

Quantification of cell-associated PSMs in wild-type, Δ<i>yqhG</i>, and complemented strains. Cell-associated PSMs were extracted from bacterial pellets using 6 M guanidine hydrochloride and quantified by reversed-phase HPLC. The Δ<i>yqhG</i> mutant exhibited a significant reduction in surface-bound PSMs compared with the wild-type strain, whereas complementation restored PSM levels to near wild-type values. Data represent mean ± s.e.m. from three independent experiments. Statistical analysis was performed using one-way ANOVA; *<i>P</i> < 0.05, ns, not significant.
-н: Jianhua Liao (1393228)
Хэвлэсэн: (2025)

Surface motility of the <i>yqhG</i> mutant.
-н: Jianhua Liao (1393228)
Хэвлэсэн: (2025)

Membrane integrity in the <i>yqhG</i> mutant.
-н: Jianhua Liao (1393228)
Хэвлэсэн: (2025)

Biofilm formation in MRSA strains.
-н: Jianhua Liao (1393228)
Хэвлэсэн: (2025)

YqhG is required for systemic virulence but not for <i>in vitro</i> growth.
-н: Jianhua Liao (1393228)
Хэвлэсэн: (2025)

Viable CFU counts following hydrogen peroxide exposure.
-н: Jianhua Liao (1393228)
Хэвлэсэн: (2025)

Amount of PSMs (μg/1mL culture) in MRSA wild-type, ΔyqhG, and complemented strains.
-н: Jianhua Liao (1393228)
Хэвлэсэн: (2025)

Colony diameter measurements from soft agar motility assays.
-н: Jianhua Liao (1393228)
Хэвлэсэн: (2025)

Crystal violet quantification of biofilm formation in MRSA wild-type, ΔyqhG, and complemented strains.
-н: Jianhua Liao (1393228)
Хэвлэсэн: (2025)

Spleen bacterial burden in mice infected with MRSA wild-type, ΔyqhG, and complemented strains.
-н: Jianhua Liao (1393228)
Хэвлэсэн: (2025)

Liver bacterial burden in mice infected with MRSA wild-type, ΔyqhG, and complemented strains.
-н: Jianhua Liao (1393228)
Хэвлэсэн: (2025)

Kidney bacterial burden in mice infected with MRSA wild-type, Δ<i>yqhG</i>, and complemented strains.
-н: Jianhua Liao (1393228)
Хэвлэсэн: (2025)

Heart bacterial burden in mice infected with MRSA wild-type, Δ<i>yqhG</i>, and complemented strains.
-н: Jianhua Liao (1393228)
Хэвлэсэн: (2025)

Survival data of BALB/c mice infected with MRSA wild-type, Δ<i>yqhG</i>, and complemented strains.
-н: Jianhua Liao (1393228)
Хэвлэсэн: (2025)

Optical density measurements of MRSA wild-type, Δ<i>yqhG</i>, and complemented strains during <i>in vitro</i> growth.
-н: Jianhua Liao (1393228)
Хэвлэсэн: (2025)

Table 1_Hard to jump: host shifts appear unlikely in a T4-like phage evolved in the lab.xlsx
-н: Yu Ning (588573)
Хэвлэсэн: (2025)

Supplementary file 1_Hard to jump: host shifts appear unlikely in a T4-like phage evolved in the lab.docx
-н: Yu Ning (588573)
Хэвлэсэн: (2025)

Protocatechuic Acid alleviates D-gal-induced renal senescence and injury in mice by regulating taurine metabolism
-н: Songhao Tian (22609826)
Хэвлэсэн: (2025)

Table 1_Melatonin mitigates cadmium-induced oxidative damage and modulates polysaccharide biosynthesis in Bletilla striata.xlsx
-н: Deqiong Niu (19691873)
Хэвлэсэн: (2025)

Data Sheet 1_Melatonin mitigates cadmium-induced oxidative damage and modulates polysaccharide biosynthesis in Bletilla striata.docx
-н: Deqiong Niu (19691873)
Хэвлэсэн: (2025)

Potent phenolics with antimigratory and anti-MRSA powers from fungus associated with <i>Saussurea involucrata</i>
-н: Wei-hua Yuan (22683169)
Хэвлэсэн: (2025)

Correlation network between intestinal bacterial species and differential serum metabolites.
-н: Ji-Xin Zhao (22056386)
Хэвлэсэн: (2025)

KEGG pathway enrichment analysis of differential serum metabolites.
-н: Ji-Xin Zhao (22056386)
Хэвлэсэн: (2025)

Untargeted serum metabolomics analysis before and after <i>T. gondii</i> infection.
-н: Ji-Xin Zhao (22056386)
Хэвлэсэн: (2025)

Functional analysis of the intestinal microbial community following <i>T. gondii</i> infection.
-н: Ji-Xin Zhao (22056386)
Хэвлэсэн: (2025)

Composition of intestinal microbial communities in the large intestine of rats before and after <i>T. gondii</i> infection.
-н: Ji-Xin Zhao (22056386)
Хэвлэсэн: (2025)

Composition of intestinal microbial communities in the small intestine of rats before and after <i>T. gondii</i> infection.
-н: Ji-Xin Zhao (22056386)
Хэвлэсэн: (2025)

Effects of <i>T. gondii</i> infection on rat gut microbiota diversity.
-н: Ji-Xin Zhao (22056386)
Хэвлэсэн: (2025)

Spearman correlation analysis between gut microbial taxa and circulating metabolites, detailing correlation coefficients, significance values, and taxon–metabolite pairings indicative of microbiome–host metabolic interactions.
-н: Ji-Xin Zhao (22056386)
Хэвлэсэн: (2025)

Pathway enrichment analysis of differential metabolites, highlighting significantly impacted metabolic pathways and associated enrichment statistics based on KEGG or other pathway databases.
-н: Ji-Xin Zhao (22056386)
Хэвлэсэн: (2025)

Differential metabolite results, listing metabolites with significant changes across treatment groups, including fold changes, and <i>p</i>-values.
-н: Ji-Xin Zhao (22056386)
Хэвлэсэн: (2025)

Orthogonal partial least squares-discriminant analysis (OPLS-DA) of serum metabolomics data.
-н: Ji-Xin Zhao (22056386)
Хэвлэсэн: (2025)

Quality control assessment of serum metabolomics data.
-н: Ji-Xin Zhao (22056386)
Хэвлэсэн: (2025)

Heatmap of enriched KEGG pathways across samples, illustrating variations in pathway abundance.
-н: Ji-Xin Zhao (22056386)
Хэвлэсэн: (2025)

Graphical abstract.
-н: Ji-Xin Zhao (22056386)
Хэвлэсэн: (2025)

The U sequence method.
-н: Pierre Angelvy (22683450)
Хэвлэсэн: (2025)

Provisional study schedule of MUSIBOT.
-н: Pierre Angelvy (22683450)
Хэвлэсэн: (2025)

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