Dopamine receptor RNAi and neuronal expression analysis in SIFa neurons.

Dopamine receptor RNAi and neuronal expression analysis in SIFa neurons.

<p><b>(A–H)</b> MD assays of flies expressing the <i>SIFaR</i><sup><i>24F06</i></sup> driver together with (A, B) <i>Dop1R1-RNAi</i>, (C, D) <i>Dop1R2-RNAi</i>, (E, F) <i>DopEcR-RNAi,</i> (G, H) <i>Dop2R-...

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محفوظ في:
التفاصيل البيبلوغرافية
المؤلف الرئيسي: Yutong Song (327091) (author)
مؤلفون آخرون: Tianmu Zhang (1731778) (author), Tae Hoon Ryu (22184665) (author), Kyle Wong (8103695) (author), Zekun Wu (9983369) (author), Yanan Wei (3768307) (author), Justine Schweizer (22184668) (author), Khoi-Nguyen Ha Nguyen (15810952) (author), Alex Kwan (22184671) (author), Xiaoli Zhang (135098) (author), Kweon Yu (145783) (author), Woo Jae Kim (15084145) (author)
منشور في: 2025
الموضوعات:
Medicine
Cell Biology
Neuroscience
Physiology
Evolutionary Biology
Sociology
Developmental Biology
Mental Health
ventral nerve cord
reduces food intake
process remain elusive
precise molecular underpinnings
increased food intake
external environmental stimuli
drosophila melanogaster </
shorter mating duration
selectively integrating inputs
reduced mating duration
longer mating duration
internal physiological cues
differentially regulate sleep
regulate energy balance
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sifa neurons interact
sifa neurons coordinate
mating duration
energy balance
sleep patterns
internal states
absorbing inputs
sifa neurons
snpf neurons
peptidergic neurons
neurons engage
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signals enable
shedding light
receive signals
neuropeptide sifamide
modifying behaviors
metabolic status
feedback loop
dependent manner
circadian timing
behavioral responses
animal ’
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الوصف
الملخص:<p><b>(A–H)</b> MD assays of flies expressing the <i>SIFaR</i><sup><i>24F06</i></sup> driver together with (A, B) <i>Dop1R1-RNAi</i>, (C, D) <i>Dop1R2-RNAi</i>, (E, F) <i>DopEcR-RNAi,</i> (G, H) <i>Dop2R-RNAi</i> (two-tailed unpaired <i>t</i> test). In all plots and statistical tests. Data are presented as mean ± s.e.m. ns = not significant (<i>p > 0.05</i>), <i>*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001</i>. Sample sizes (<i>n</i>) are indicated in the figure panels. <b>(I)</b> Control for <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3003345#pbio.3003345.g003" target="_blank">Fig 3A</a>. Male flies expressing <i>SIFaR</i><sup><i>24F06</i></sup> drivers together with <i>UAS-mCD8RFP</i> were imaged live under a fluorescent microscope with anti-GFP (green), anti-DsRed (red), and nc82 (blue) antibodies. Areas outlined by boxes are enlarged in the bottom panel. Scale bars represent 100 μm. <b>(J)</b> Flies expressing <i>LexA</i><sup><i>SIFa.PT</i></sup> with <i>TH-C-GAL4</i> drivers together with <i>UAS-stinger</i>, <i>LexAop-tdTomato.nls</i> was immunostained with anti-GFP (green), anti-RFP (red), and nc82 (blue) antibodies. Scale bars represent 100 µm. <b>(K)</b> Flies expressing <i>LexA</i><sup><i>2A-LexA</i></sup> with <i>TH-F-GAL4</i> drivers together with <i>UAS-stinger</i>, <i>LexAop-tdTomato.nls</i> was immunostained with anti-GFP (green), anti-RFP (red), and nc82 (blue) antibodies. Scale bars represent 100 µm. Underlying data for all graphs can be found in file <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3003345#pbio.3003345.s016" target="_blank">S1 Data</a>.</p> <p>(TIF)</p>

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