Representative results of PCR-RFLP analysis showing specific mutations of Omicron sublineages.

A: PCR amplification and NlaIII digestion of OFR1b gene (aa 254-303) detecting the Y264H mutation characterizing BQ.1 sublineages in lanes 1, 2, 4 and 5. B: PCR amplification and BfaI digestion of S gene (aa 412-475) detecting the two successive mutatio...

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Main Author:	Wajdi Ayadi (10315724) (author)
Other Authors:	Fahmi Smaoui (21246349) (author), Saba Gargouri (14325678) (author), Sana Ferjani (14325669) (author), Zaineb Hamzaoui (21246352) (author), Awatef Taktak (21246355) (author), Amel Chtourou (21246358) (author), Houda Skouri-Gargouri (21246361) (author), Azza Hadj Sassi (21246364) (author), Mouna Ben Sassi (21246367) (author), Sameh Trabelsi (5100557) (author), Ali Gargouri (3515591) (author), Ilhem Boutiba-Ben Boubaker (11740485) (author), Héla Karray-Hakim (21246370) (author), Raja Mokdad-Gargouri (11442455) (author), Lamia Feki-Berrajah (21246373) (author)
Published:	2025
Subjects:	Microbiology Genetics Molecular Biology Biotechnology Immunology Infectious Diseases Virology Biological Sciences not elsewhere classified Information Systems not elsewhere classified effective infection control 227 confirmed covid new relevant mutations especially omicron variants xlink "> monitoring verifying key sars simple approaches used existing lineages ba deletions &# 916 )- qpcr detection &# 916 mutations y264h cov2 variants additional variants xbb variant valuable tools specific qpcr sequencing results sequenced cases september 2022 second stage screening tests screening approach rigorously assessed restriction analysis report lessons recent sublineages rapid genotyping rapid detection quickly replaced proven methods predominant variant october 2022 methods appear major concern including ch identifying sars first stage findings highlight developing countries april 2023 analytical performance 7 %). 7 %) 62 ). 38 cases 19 cases 165 cases 14 cases 115 ),
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Summary:	<p><b>A</b>: PCR amplification and NlaIII digestion of OFR1b gene (aa 254-303) detecting the Y264H mutation characterizing BQ.1 sublineages in lanes 1, 2, 4 and 5. <b>B</b>: PCR amplification and BfaI digestion of S gene (aa 412-475) detecting the two successive mutations V445P and G446S characterizing XBB sublineages in lanes 6, 7, 8, 9, 12 and 13. <b>C</b>: PCR amplification and MspI/DdeI digestion of S gene (aa 412-475) detecting the two mutations K444X (T, R, N or M) and L452Q of the BA.5 descendent sublineages in lanes 14, 15 and 16; the N460K (AAG) mutation characterizing the parental BA.2.75 sublineage in lanes 17 and 18; the three mutations K444X (T, R, N or <b>M)</b>, L452Q and N460K (AAG) characterizing CH1.1. sublineage in lane 19. Lane L1: 50 bp DNA ladder. Lane L2: 100 bp DNA ladder.</p>

Representative results of PCR-RFLP analysis showing specific mutations of Omicron sublineages.

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