Viral gene expression within productively infected (GFP+) cells is increased in the presence of Vpr and vorinostat (VOR).

A) Top panel (cis Vpr expression): schematic of workflow with infection with Vpr revertant vs. Vpr-null virus (HIV-GFP) and lower panel (trans Vpr expression): co-infection with Vpr-mCherry lentivirus or mCherry lentivirus and HIV-GFP (created with Biorender.com). <b&g...

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Bibliografski detalji
Glavni autor:	Catherine A. Lewis (22177707) (author)
Daljnji autori:	David M. Margolis (244046) (author), Edward P. Browne (8913893) (author)
Izdano:	2025
Teme:	Cell Biology Molecular Biology Biotechnology Immunology Developmental Biology Cancer Infectious Diseases Virology vorinostat potently enhances viral factors shape viral factors interact sup >+</ sup specific viral factors div >< p critical cellular factors 1 reporter strains 1 accessory proteins 1 accessory protein reservoir formation selects preventing reservoir formation regulate latency establishment 1 latency results 1 latency establishment prevents hiv transmission cure hiv infection vpr &# 8217 latent viral reservoir 1 protein vpr whether hdacs interact cell latency model host cell factors promoting hiv expression central memory cd4 latent reservoir central memory 1 entry vpr expression new latency cell subtypes cell death latent proviruses single hiv individual hiv allow hiv vpr possesses vpr plays significant increase recently shown promoting activity primary cd4 mediated restriction induced apoptosis histone deacetylases findings suggest dramatically improved clinical prognosis binding domain antiretroviral therapy
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Sažetak:	<p><b>A)</b> Top panel (cis Vpr expression): schematic of workflow with infection with Vpr revertant vs. Vpr-null virus (HIV-GFP) and lower panel (trans Vpr expression): co-infection with Vpr-mCherry lentivirus or mCherry lentivirus and HIV-GFP (created with Biorender.com). <b>B)</b> Top panel: western blot for Vpr expression in 293T cells transfected with HIV-GFP, Vpr revertant plasmid, mCherry lentivirus plasmid, or Vpr-mCherry lentivrus plasmid. Lower panel: representative dot plot of co-infected CD4<sup>+</sup> T cells. C-D) Representative quantification of GFP MFI over 21d in C) cis Vpr infection model (n = 9) and D) trans Vpr infection model (n = 6). Representative dot plots with and without VOR below. %GFP<sup>+</sup> cells is indicated on graph. GFP MFI can be visualized as lateral movement of cells within GFP<sup>+</sup> gate. E-F) Left: Representative GFP MFI quantification by flow cytometry and (right) gag unspliced transcript quantification by qPCR in E) cis Vpr infection model (n = 2) and F) trans Vpr infection model (n = 3 donors).</p>

Viral gene expression within productively infected (GFP<sup>+</sup>) cells is increased in the presence of Vpr and vorinostat (VOR).

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