Characterization of the Hog1-associated cytoplasmic foci in response to FA treatment, after Co-IP.
<p>Foci content was subjected to Co-IP with GFP-Trap magnetic beads. Proteins significantly enriched in FA foci (P-value < 0.05, log2 fold-change > 1) are marked in orange. Puf2 (UniProt ID: N4WW71) in red, and RNA recognition motif (RRM)-containing proteins in blue. <b>A)</b>...
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2025
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| 要約: | <p>Foci content was subjected to Co-IP with GFP-Trap magnetic beads. Proteins significantly enriched in FA foci (P-value < 0.05, log2 fold-change > 1) are marked in orange. Puf2 (UniProt ID: N4WW71) in red, and RNA recognition motif (RRM)-containing proteins in blue. <b>A)</b> Volcano plot comparing proteins enriched in Hog1 foci to the DMSO control. <b>B)</b> Volcano plot comparing proteins enriched in Hog1 foci to untagged control. <b>C)</b> Venn diagram showing the overlap of significant proteins enriched in FA foci compared to DMSO (red circle) and untagged controls (blue circle). <b>D)</b> Sankey plot of GO terms enriched in the 90 shared proteins identified in FA foci. GO terms (Molecular Function, Cellular Component, Biological Process) were analyzed using DAVID (v2023q2). Circle size represents the number of enriched proteins, with Puf2 highlighted by an arrowhead. Fold enrichment is shown on the x-axis. <b>E)</b> Multi-panel scatter plots visualizing proteomic differences across experimental conditions. Each panel represents pairwise comparisons of proteomic differences between: Total proteome Co-IP, SGEF Co-IP (FA-DM), SGEF Co-IP (Hog1:GFP-Untagged C4). Points are colored according to the SGEF log2 FC values using an orange-to-gray-to-purple gradient, where orange represents the lowest values and purple the highest. Highlighted proteins, Puf2 and eIF2 (UniProt ID: N4WKP0), are marked.</p> |
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