Characterization of the Hog1-associated cytoplasmic foci in response to FA treatment, after Co-IP.
<p>Foci content was subjected to Co-IP with GFP-Trap magnetic beads. Proteins significantly enriched in FA foci (P-value < 0.05, log2 fold-change > 1) are marked in orange. Puf2 (UniProt ID: N4WW71) in red, and RNA recognition motif (RRM)-containing proteins in blue. <b>A)</b>...
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2025
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| _version_ | 1851481555151618048 |
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| author | Rina Zuchman (409382) |
| author2 | Roni Koren (634297) Tamar Ziv (168326) Yael Lupu-Haber (1937800) Nitsan Dahan (5895893) Ofri Levi (6711023) Benjamin A. Horwitz (11921937) |
| author2_role | author author author author author author |
| author_facet | Rina Zuchman (409382) Roni Koren (634297) Tamar Ziv (168326) Yael Lupu-Haber (1937800) Nitsan Dahan (5895893) Ofri Levi (6711023) Benjamin A. Horwitz (11921937) |
| author_role | author |
| dc.creator.none.fl_str_mv | Rina Zuchman (409382) Roni Koren (634297) Tamar Ziv (168326) Yael Lupu-Haber (1937800) Nitsan Dahan (5895893) Ofri Levi (6711023) Benjamin A. Horwitz (11921937) |
| dc.date.none.fl_str_mv | 2025-10-30T17:46:13Z |
| dc.identifier.none.fl_str_mv | 10.1371/journal.ppat.1013620.g003 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/figure/Characterization_of_the_Hog1-associated_cytoplasmic_foci_in_response_to_FA_treatment_after_Co-IP_/30493641 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biochemistry Microbiology Cell Biology Genetics Molecular Biology Ecology Plant Biology Biological Sciences not elsewhere classified translation initiation factors phenolic compound abundant monosaccharide transporter gene live imaging confirmed collectively promote survival affinity purification bait puf2 fluorescent fusion cochliobolus heterostrophus </ subcellular fraction enriched stress might cause downstream gene expression div >< p host defense interferes mst1 </ host defense fluorescent foci thus relevant plant host osmotic stress nuclear localization mostly distinct mitochondrial proteins maize pathogen host plant fungal stress fragmented mitochondria ferulic acid dependent sub containing granules containing foci binding proteins activated mapk |
| dc.title.none.fl_str_mv | Characterization of the Hog1-associated cytoplasmic foci in response to FA treatment, after Co-IP. |
| dc.type.none.fl_str_mv | Image Figure info:eu-repo/semantics/publishedVersion image |
| description | <p>Foci content was subjected to Co-IP with GFP-Trap magnetic beads. Proteins significantly enriched in FA foci (P-value < 0.05, log2 fold-change > 1) are marked in orange. Puf2 (UniProt ID: N4WW71) in red, and RNA recognition motif (RRM)-containing proteins in blue. <b>A)</b> Volcano plot comparing proteins enriched in Hog1 foci to the DMSO control. <b>B)</b> Volcano plot comparing proteins enriched in Hog1 foci to untagged control. <b>C)</b> Venn diagram showing the overlap of significant proteins enriched in FA foci compared to DMSO (red circle) and untagged controls (blue circle). <b>D)</b> Sankey plot of GO terms enriched in the 90 shared proteins identified in FA foci. GO terms (Molecular Function, Cellular Component, Biological Process) were analyzed using DAVID (v2023q2). Circle size represents the number of enriched proteins, with Puf2 highlighted by an arrowhead. Fold enrichment is shown on the x-axis. <b>E)</b> Multi-panel scatter plots visualizing proteomic differences across experimental conditions. Each panel represents pairwise comparisons of proteomic differences between: Total proteome Co-IP, SGEF Co-IP (FA-DM), SGEF Co-IP (Hog1:GFP-Untagged C4). Points are colored according to the SGEF log2 FC values using an orange-to-gray-to-purple gradient, where orange represents the lowest values and purple the highest. Highlighted proteins, Puf2 and eIF2 (UniProt ID: N4WKP0), are marked.</p> |
| eu_rights_str_mv | openAccess |
| id | Manara_8f03f45aae6f111a6ebf0e2aacad5cbc |
| identifier_str_mv | 10.1371/journal.ppat.1013620.g003 |
| network_acronym_str | Manara |
| network_name_str | ManaraRepo |
| oai_identifier_str | oai:figshare.com:article/30493641 |
| publishDate | 2025 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Characterization of the Hog1-associated cytoplasmic foci in response to FA treatment, after Co-IP.Rina Zuchman (409382)Roni Koren (634297)Tamar Ziv (168326)Yael Lupu-Haber (1937800)Nitsan Dahan (5895893)Ofri Levi (6711023)Benjamin A. Horwitz (11921937)BiochemistryMicrobiologyCell BiologyGeneticsMolecular BiologyEcologyPlant BiologyBiological Sciences not elsewhere classifiedtranslation initiation factorsphenolic compound abundantmonosaccharide transporter genelive imaging confirmedcollectively promote survivalaffinity purification baitpuf2 fluorescent fusioncochliobolus heterostrophus </subcellular fraction enrichedstress might causedownstream gene expressiondiv >< phost defense interferesmst1 </host defensefluorescent focithus relevantplant hostosmotic stressnuclear localizationmostly distinctmitochondrial proteinsmaize pathogenhost plantfungal stressfragmented mitochondriaferulic aciddependent subcontaining granulescontaining focibinding proteinsactivated mapk<p>Foci content was subjected to Co-IP with GFP-Trap magnetic beads. Proteins significantly enriched in FA foci (P-value < 0.05, log2 fold-change > 1) are marked in orange. Puf2 (UniProt ID: N4WW71) in red, and RNA recognition motif (RRM)-containing proteins in blue. <b>A)</b> Volcano plot comparing proteins enriched in Hog1 foci to the DMSO control. <b>B)</b> Volcano plot comparing proteins enriched in Hog1 foci to untagged control. <b>C)</b> Venn diagram showing the overlap of significant proteins enriched in FA foci compared to DMSO (red circle) and untagged controls (blue circle). <b>D)</b> Sankey plot of GO terms enriched in the 90 shared proteins identified in FA foci. GO terms (Molecular Function, Cellular Component, Biological Process) were analyzed using DAVID (v2023q2). Circle size represents the number of enriched proteins, with Puf2 highlighted by an arrowhead. Fold enrichment is shown on the x-axis. <b>E)</b> Multi-panel scatter plots visualizing proteomic differences across experimental conditions. Each panel represents pairwise comparisons of proteomic differences between: Total proteome Co-IP, SGEF Co-IP (FA-DM), SGEF Co-IP (Hog1:GFP-Untagged C4). Points are colored according to the SGEF log2 FC values using an orange-to-gray-to-purple gradient, where orange represents the lowest values and purple the highest. Highlighted proteins, Puf2 and eIF2 (UniProt ID: N4WKP0), are marked.</p>2025-10-30T17:46:13ZImageFigureinfo:eu-repo/semantics/publishedVersionimage10.1371/journal.ppat.1013620.g003https://figshare.com/articles/figure/Characterization_of_the_Hog1-associated_cytoplasmic_foci_in_response_to_FA_treatment_after_Co-IP_/30493641CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/304936412025-10-30T17:46:13Z |
| spellingShingle | Characterization of the Hog1-associated cytoplasmic foci in response to FA treatment, after Co-IP. Rina Zuchman (409382) Biochemistry Microbiology Cell Biology Genetics Molecular Biology Ecology Plant Biology Biological Sciences not elsewhere classified translation initiation factors phenolic compound abundant monosaccharide transporter gene live imaging confirmed collectively promote survival affinity purification bait puf2 fluorescent fusion cochliobolus heterostrophus </ subcellular fraction enriched stress might cause downstream gene expression div >< p host defense interferes mst1 </ host defense fluorescent foci thus relevant plant host osmotic stress nuclear localization mostly distinct mitochondrial proteins maize pathogen host plant fungal stress fragmented mitochondria ferulic acid dependent sub containing granules containing foci binding proteins activated mapk |
| status_str | publishedVersion |
| title | Characterization of the Hog1-associated cytoplasmic foci in response to FA treatment, after Co-IP. |
| title_full | Characterization of the Hog1-associated cytoplasmic foci in response to FA treatment, after Co-IP. |
| title_fullStr | Characterization of the Hog1-associated cytoplasmic foci in response to FA treatment, after Co-IP. |
| title_full_unstemmed | Characterization of the Hog1-associated cytoplasmic foci in response to FA treatment, after Co-IP. |
| title_short | Characterization of the Hog1-associated cytoplasmic foci in response to FA treatment, after Co-IP. |
| title_sort | Characterization of the Hog1-associated cytoplasmic foci in response to FA treatment, after Co-IP. |
| topic | Biochemistry Microbiology Cell Biology Genetics Molecular Biology Ecology Plant Biology Biological Sciences not elsewhere classified translation initiation factors phenolic compound abundant monosaccharide transporter gene live imaging confirmed collectively promote survival affinity purification bait puf2 fluorescent fusion cochliobolus heterostrophus </ subcellular fraction enriched stress might cause downstream gene expression div >< p host defense interferes mst1 </ host defense fluorescent foci thus relevant plant host osmotic stress nuclear localization mostly distinct mitochondrial proteins maize pathogen host plant fungal stress fragmented mitochondria ferulic acid dependent sub containing granules containing foci binding proteins activated mapk |