Table 1_Immune responses in pulmonary sarcoidosis following COVID-19.docx

Background/objectives<p>The complex interplay between sarcoidosis and COVID-19 remains an important area of research, since COVID-19 leads to long-term changes in the immune system. However, COVID-19 is often followed by autoimmune diseases, including newly manifesting sarcoidosis. The goal of...

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Tác giả chính: Anna Starshinova (22571846) (author)
Tác giả khác: Igor Kudryavtsev (5741774) (author), Artem Rubinstein (22679072) (author), Tatiana Akisheva (22679075) (author), Alexey Golovkin (6178088) (author), Zoia Korobova (14593420) (author), Anastasia Kulpina (22679078) (author), Dmitry Kudlay (22571849) (author)
Được phát hành: 2025
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author Anna Starshinova (22571846)
author2 Igor Kudryavtsev (5741774)
Artem Rubinstein (22679072)
Tatiana Akisheva (22679075)
Alexey Golovkin (6178088)
Zoia Korobova (14593420)
Anastasia Kulpina (22679078)
Dmitry Kudlay (22571849)
author2_role author
author
author
author
author
author
author
author_facet Anna Starshinova (22571846)
Igor Kudryavtsev (5741774)
Artem Rubinstein (22679072)
Tatiana Akisheva (22679075)
Alexey Golovkin (6178088)
Zoia Korobova (14593420)
Anastasia Kulpina (22679078)
Dmitry Kudlay (22571849)
author_role author
dc.creator.none.fl_str_mv Anna Starshinova (22571846)
Igor Kudryavtsev (5741774)
Artem Rubinstein (22679072)
Tatiana Akisheva (22679075)
Alexey Golovkin (6178088)
Zoia Korobova (14593420)
Anastasia Kulpina (22679078)
Dmitry Kudlay (22571849)
dc.date.none.fl_str_mv 2025-11-25T06:15:49Z
dc.identifier.none.fl_str_mv 10.3389/fimmu.2025.1614461.s001
dc.relation.none.fl_str_mv https://figshare.com/articles/dataset/Table_1_Immune_responses_in_pulmonary_sarcoidosis_following_COVID-19_docx/30703736
dc.rights.none.fl_str_mv CC BY 4.0
info:eu-repo/semantics/openAccess
dc.subject.none.fl_str_mv Genetic Immunology
autoimmunity
granulomatous diseases
follicular Th cells
post-COVID-19
pathogenesis
sarcoidosis
Th subsets
Th17
dc.title.none.fl_str_mv Table 1_Immune responses in pulmonary sarcoidosis following COVID-19.docx
dc.type.none.fl_str_mv Dataset
info:eu-repo/semantics/publishedVersion
dataset
description Background/objectives<p>The complex interplay between sarcoidosis and COVID-19 remains an important area of research, since COVID-19 leads to long-term changes in the immune system. However, COVID-19 is often followed by autoimmune diseases, including newly manifesting sarcoidosis. The goal of this study is to characterize CD4+ T cell subsets, playing a pivotal role in the regulation of innate and adaptive immunity, in the peripheral blood of patients with sarcoidosis after COVID-19.</p>Methods<p>The peripheral blood samples from patients with sarcoidosis (n = 61) were studied. We divided patients into two distinct groups: sarcoidosis patients with no history of COVID-19 (n= 30) and COVID-19 convalescent patients with sarcoidosis within 12–24 weeks after recovery (n = 31). Healthy controls (n = 40) were similar in terms of age and sex to patients with sarcoidosis. Immunophenotyping of peripheral blood cells was performed using a ten-color flow cytometry.</p>Results<p>Sarcoidosis patients with COVID-19 history had higher levels of T-helper cells (Th) when compared to COVID-19 naïve patients with sarcoidosis, but lower levels when compared to healthy controls. In COVID-19 convalescent patients with sarcoidosis, we noted higher absolute numbers and percentages of CD45RA–CCR7– and CD45RA+CCR7– cells within Th subset. Among COVID-19 convalescent patients with sarcoidosis we also found higher levels of T helper 1 cells and T helper 2 cells (with CXCR5–CCR6–CXCR3+CCR4– and CXCR5–CCR6–CXCR3–CCR4+ phenotypes, respectively) when compared to other groups. We also noted a statistically significant increase in central memory CXCR5+CCR6–CXCR3– follicular Th cells, as wells as effector memory CXCR5+CCR6–CXCR3– and CXCR5+CCR6+CXCR3– follicular Th cells in both groups of patients with sarcoidosis vs. healthy controls.</p>Conclusions<p>Our study demonstrated Th cells imbalance in patients with sarcoidosis and COVID-19 history. These findings suggest possible clinical and visual progression of chronic lung sarcoidosis in COVID-19 convalescent patients.</p>
eu_rights_str_mv openAccess
id Manara_90984427d108adbcdf1e75e4cbd5233a
identifier_str_mv 10.3389/fimmu.2025.1614461.s001
network_acronym_str Manara
network_name_str ManaraRepo
oai_identifier_str oai:figshare.com:article/30703736
publishDate 2025
repository.mail.fl_str_mv
repository.name.fl_str_mv
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rights_invalid_str_mv CC BY 4.0
spelling Table 1_Immune responses in pulmonary sarcoidosis following COVID-19.docxAnna Starshinova (22571846)Igor Kudryavtsev (5741774)Artem Rubinstein (22679072)Tatiana Akisheva (22679075)Alexey Golovkin (6178088)Zoia Korobova (14593420)Anastasia Kulpina (22679078)Dmitry Kudlay (22571849)Genetic Immunologyautoimmunitygranulomatous diseasesfollicular Th cellspost-COVID-19pathogenesissarcoidosisTh subsetsTh17Background/objectives<p>The complex interplay between sarcoidosis and COVID-19 remains an important area of research, since COVID-19 leads to long-term changes in the immune system. However, COVID-19 is often followed by autoimmune diseases, including newly manifesting sarcoidosis. The goal of this study is to characterize CD4+ T cell subsets, playing a pivotal role in the regulation of innate and adaptive immunity, in the peripheral blood of patients with sarcoidosis after COVID-19.</p>Methods<p>The peripheral blood samples from patients with sarcoidosis (n = 61) were studied. We divided patients into two distinct groups: sarcoidosis patients with no history of COVID-19 (n= 30) and COVID-19 convalescent patients with sarcoidosis within 12–24 weeks after recovery (n = 31). Healthy controls (n = 40) were similar in terms of age and sex to patients with sarcoidosis. Immunophenotyping of peripheral blood cells was performed using a ten-color flow cytometry.</p>Results<p>Sarcoidosis patients with COVID-19 history had higher levels of T-helper cells (Th) when compared to COVID-19 naïve patients with sarcoidosis, but lower levels when compared to healthy controls. In COVID-19 convalescent patients with sarcoidosis, we noted higher absolute numbers and percentages of CD45RA–CCR7– and CD45RA+CCR7– cells within Th subset. Among COVID-19 convalescent patients with sarcoidosis we also found higher levels of T helper 1 cells and T helper 2 cells (with CXCR5–CCR6–CXCR3+CCR4– and CXCR5–CCR6–CXCR3–CCR4+ phenotypes, respectively) when compared to other groups. We also noted a statistically significant increase in central memory CXCR5+CCR6–CXCR3– follicular Th cells, as wells as effector memory CXCR5+CCR6–CXCR3– and CXCR5+CCR6+CXCR3– follicular Th cells in both groups of patients with sarcoidosis vs. healthy controls.</p>Conclusions<p>Our study demonstrated Th cells imbalance in patients with sarcoidosis and COVID-19 history. These findings suggest possible clinical and visual progression of chronic lung sarcoidosis in COVID-19 convalescent patients.</p>2025-11-25T06:15:49ZDatasetinfo:eu-repo/semantics/publishedVersiondataset10.3389/fimmu.2025.1614461.s001https://figshare.com/articles/dataset/Table_1_Immune_responses_in_pulmonary_sarcoidosis_following_COVID-19_docx/30703736CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/307037362025-11-25T06:15:49Z
spellingShingle Table 1_Immune responses in pulmonary sarcoidosis following COVID-19.docx
Anna Starshinova (22571846)
Genetic Immunology
autoimmunity
granulomatous diseases
follicular Th cells
post-COVID-19
pathogenesis
sarcoidosis
Th subsets
Th17
status_str publishedVersion
title Table 1_Immune responses in pulmonary sarcoidosis following COVID-19.docx
title_full Table 1_Immune responses in pulmonary sarcoidosis following COVID-19.docx
title_fullStr Table 1_Immune responses in pulmonary sarcoidosis following COVID-19.docx
title_full_unstemmed Table 1_Immune responses in pulmonary sarcoidosis following COVID-19.docx
title_short Table 1_Immune responses in pulmonary sarcoidosis following COVID-19.docx
title_sort Table 1_Immune responses in pulmonary sarcoidosis following COVID-19.docx
topic Genetic Immunology
autoimmunity
granulomatous diseases
follicular Th cells
post-COVID-19
pathogenesis
sarcoidosis
Th subsets
Th17