Table 3_Metabolome profiling by untargeted metabolomics and biomarker panel selection using machine-learning for patients in different stages of peripheral neuropathy induced by oxaliplatin.xlsx

Background<p>Oxaliplatin-induced peripheral neuropathy (OIPN) poses a significant challenge for patients with colorectal tumor, often resulting in treatment interruption or discontinuation and subsequent treatment failure. Herein, a longitudinal untargeted metabolomic study to reveal the metab...

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Main Author: Yu-jiao Hua (22274131) (author)
Other Authors: Ying Zhang (40767) (author), Rui-Rong Wu (22274134) (author), Juan Lv (3398315) (author), Yan Zhang (8098) (author), Yan-yan Chen (1874491) (author), Yong-juan Ding (22274137) (author), Jing-hua Chen (22274140) (author)
Published: 2025
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_version_ 1852016460652609536
author Yu-jiao Hua (22274131)
author2 Ying Zhang (40767)
Rui-Rong Wu (22274134)
Juan Lv (3398315)
Yan Zhang (8098)
Yan-yan Chen (1874491)
Yong-juan Ding (22274137)
Jing-hua Chen (22274140)
author2_role author
author
author
author
author
author
author
author_facet Yu-jiao Hua (22274131)
Ying Zhang (40767)
Rui-Rong Wu (22274134)
Juan Lv (3398315)
Yan Zhang (8098)
Yan-yan Chen (1874491)
Yong-juan Ding (22274137)
Jing-hua Chen (22274140)
author_role author
dc.creator.none.fl_str_mv Yu-jiao Hua (22274131)
Ying Zhang (40767)
Rui-Rong Wu (22274134)
Juan Lv (3398315)
Yan Zhang (8098)
Yan-yan Chen (1874491)
Yong-juan Ding (22274137)
Jing-hua Chen (22274140)
dc.date.none.fl_str_mv 2025-09-19T04:12:54Z
dc.identifier.none.fl_str_mv 10.3389/fonc.2025.1617207.s003
dc.relation.none.fl_str_mv https://figshare.com/articles/dataset/Table_3_Metabolome_profiling_by_untargeted_metabolomics_and_biomarker_panel_selection_using_machine-learning_for_patients_in_different_stages_of_peripheral_neuropathy_induced_by_oxaliplatin_xlsx/30162787
dc.rights.none.fl_str_mv CC BY 4.0
info:eu-repo/semantics/openAccess
dc.subject.none.fl_str_mv Oncology and Carcinogenesis not elsewhere classified
oxaliplatin-induced peripheral neuropathy
biomarkers
untargeted metabolomics
machine-learning
colorectal cancer
dc.title.none.fl_str_mv Table 3_Metabolome profiling by untargeted metabolomics and biomarker panel selection using machine-learning for patients in different stages of peripheral neuropathy induced by oxaliplatin.xlsx
dc.type.none.fl_str_mv Dataset
info:eu-repo/semantics/publishedVersion
dataset
description Background<p>Oxaliplatin-induced peripheral neuropathy (OIPN) poses a significant challenge for patients with colorectal tumor, often resulting in treatment interruption or discontinuation and subsequent treatment failure. Herein, a longitudinal untargeted metabolomic study to reveal the metabolomic profiles and biomarkers associated with the progression of OIPN.</p>Methods<p>A prospective cohort of 129 colorectal cancer patients receiving oxaliplatin-based chemotherapy was stratified into four OIPN severity grades (Level 0-3). Serum samples underwent untargeted LC-MS/MS metabolomic analysis, detecting 521 metabolites. Multivariate statistical models and SHAP-guided random forest algorithms were employed to prioritize biomarkers. Machine learning validation included six classifiers assessed via ROC-AUC.</p>Results<p>The cumulative dose of Oxaliplatin chemotherapy plays an important role in OIPN. At the same time, our findings implied that the occurrence of OIPN may be associated with the progression of the disease and the patients’ tumor markers (CEA, CA19-9, CA72-4), as well as immune response and inflammation (ANC, PLT), and metabolic and liver function abnormalities (GGT and UA) (P<0.05).Multivariate statistical analysis combined with SHAP-guided machine learning identified six biomarkers, including thiabendazole, 1-methylxanthine, imidazol-5-yl-pyruvate, 5-hydroxypentanoic acid, spermidine, and 4’-oxolividamine that consistently distinguished OIPN patients (Level 1-3) from non-OIPN controls (Level 0). Machine learning models, validated across six classifiers, demonstrated near-perfect discrimination for early-stage OIPN (AUC nearly 1). However, differentiation between intermediate OIPN grades (Level 1 vs 2, Level 1 vs 3, Level 2 vs 3) yielded lower predictive accuracy (AUC: 0.549–0.843), likely due to cohort size limitations and reliance on subjective sensory-based grading. Pathway enrichment analysis highlighted dysregulation in ABC transporters, central carbon metabolism in cancer, amino acid metabolism, and linoleic acid metabolism, suggesting potential roles in OIPN pathogenesis.</p>Conclusions<p>These findings suggest that the selected biomarkers could serve as a foundation for the prediction and management of OIPN in colorectal cancer patients.</p>
eu_rights_str_mv openAccess
id Manara_944c8c331ec00dfb9397dea4cae23f30
identifier_str_mv 10.3389/fonc.2025.1617207.s003
network_acronym_str Manara
network_name_str ManaraRepo
oai_identifier_str oai:figshare.com:article/30162787
publishDate 2025
repository.mail.fl_str_mv
repository.name.fl_str_mv
repository_id_str
rights_invalid_str_mv CC BY 4.0
spelling Table 3_Metabolome profiling by untargeted metabolomics and biomarker panel selection using machine-learning for patients in different stages of peripheral neuropathy induced by oxaliplatin.xlsxYu-jiao Hua (22274131)Ying Zhang (40767)Rui-Rong Wu (22274134)Juan Lv (3398315)Yan Zhang (8098)Yan-yan Chen (1874491)Yong-juan Ding (22274137)Jing-hua Chen (22274140)Oncology and Carcinogenesis not elsewhere classifiedoxaliplatin-induced peripheral neuropathybiomarkersuntargeted metabolomicsmachine-learningcolorectal cancerBackground<p>Oxaliplatin-induced peripheral neuropathy (OIPN) poses a significant challenge for patients with colorectal tumor, often resulting in treatment interruption or discontinuation and subsequent treatment failure. Herein, a longitudinal untargeted metabolomic study to reveal the metabolomic profiles and biomarkers associated with the progression of OIPN.</p>Methods<p>A prospective cohort of 129 colorectal cancer patients receiving oxaliplatin-based chemotherapy was stratified into four OIPN severity grades (Level 0-3). Serum samples underwent untargeted LC-MS/MS metabolomic analysis, detecting 521 metabolites. Multivariate statistical models and SHAP-guided random forest algorithms were employed to prioritize biomarkers. Machine learning validation included six classifiers assessed via ROC-AUC.</p>Results<p>The cumulative dose of Oxaliplatin chemotherapy plays an important role in OIPN. At the same time, our findings implied that the occurrence of OIPN may be associated with the progression of the disease and the patients’ tumor markers (CEA, CA19-9, CA72-4), as well as immune response and inflammation (ANC, PLT), and metabolic and liver function abnormalities (GGT and UA) (P<0.05).Multivariate statistical analysis combined with SHAP-guided machine learning identified six biomarkers, including thiabendazole, 1-methylxanthine, imidazol-5-yl-pyruvate, 5-hydroxypentanoic acid, spermidine, and 4’-oxolividamine that consistently distinguished OIPN patients (Level 1-3) from non-OIPN controls (Level 0). Machine learning models, validated across six classifiers, demonstrated near-perfect discrimination for early-stage OIPN (AUC nearly 1). However, differentiation between intermediate OIPN grades (Level 1 vs 2, Level 1 vs 3, Level 2 vs 3) yielded lower predictive accuracy (AUC: 0.549–0.843), likely due to cohort size limitations and reliance on subjective sensory-based grading. Pathway enrichment analysis highlighted dysregulation in ABC transporters, central carbon metabolism in cancer, amino acid metabolism, and linoleic acid metabolism, suggesting potential roles in OIPN pathogenesis.</p>Conclusions<p>These findings suggest that the selected biomarkers could serve as a foundation for the prediction and management of OIPN in colorectal cancer patients.</p>2025-09-19T04:12:54ZDatasetinfo:eu-repo/semantics/publishedVersiondataset10.3389/fonc.2025.1617207.s003https://figshare.com/articles/dataset/Table_3_Metabolome_profiling_by_untargeted_metabolomics_and_biomarker_panel_selection_using_machine-learning_for_patients_in_different_stages_of_peripheral_neuropathy_induced_by_oxaliplatin_xlsx/30162787CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/301627872025-09-19T04:12:54Z
spellingShingle Table 3_Metabolome profiling by untargeted metabolomics and biomarker panel selection using machine-learning for patients in different stages of peripheral neuropathy induced by oxaliplatin.xlsx
Yu-jiao Hua (22274131)
Oncology and Carcinogenesis not elsewhere classified
oxaliplatin-induced peripheral neuropathy
biomarkers
untargeted metabolomics
machine-learning
colorectal cancer
status_str publishedVersion
title Table 3_Metabolome profiling by untargeted metabolomics and biomarker panel selection using machine-learning for patients in different stages of peripheral neuropathy induced by oxaliplatin.xlsx
title_full Table 3_Metabolome profiling by untargeted metabolomics and biomarker panel selection using machine-learning for patients in different stages of peripheral neuropathy induced by oxaliplatin.xlsx
title_fullStr Table 3_Metabolome profiling by untargeted metabolomics and biomarker panel selection using machine-learning for patients in different stages of peripheral neuropathy induced by oxaliplatin.xlsx
title_full_unstemmed Table 3_Metabolome profiling by untargeted metabolomics and biomarker panel selection using machine-learning for patients in different stages of peripheral neuropathy induced by oxaliplatin.xlsx
title_short Table 3_Metabolome profiling by untargeted metabolomics and biomarker panel selection using machine-learning for patients in different stages of peripheral neuropathy induced by oxaliplatin.xlsx
title_sort Table 3_Metabolome profiling by untargeted metabolomics and biomarker panel selection using machine-learning for patients in different stages of peripheral neuropathy induced by oxaliplatin.xlsx
topic Oncology and Carcinogenesis not elsewhere classified
oxaliplatin-induced peripheral neuropathy
biomarkers
untargeted metabolomics
machine-learning
colorectal cancer