Determination of the limit of detection and the analytical sensibility.

Determination of the limit of detection and the analytical sensibility.

<p>A-ELISA to detect DENV1-4 NS1 at different concentrations to determine the limit of detection of the optimized cNb-ELISA. The developed detection system has variable LOD for the different DENV NS1. B-Estimation of the analytical sensitivity of the cNb-ELISA, absorbance values obtained based...

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Bibliographic Details
Main Author: María Florencia Pavan (22538063) (author)
Other Authors: Cristian Miguel Malnero (22538066) (author), María Emilia Mora Alvarado (22538069) (author), María Camila Carzoglio (22538072) (author), Yesica Paredes Rojas (22538075) (author), Agostina Bruno (9708143) (author), Laura Perez Vidakovics (14372943) (author), Leo Hanke (14245409) (author), Alejandro Castello (11822201) (author), Gerald McInerney (167172) (author), Cybele Carina García (22538078) (author), Viviana Parreño (409570) (author), Lorena Itatí Ibañez (22538081) (author)
Published: 2025
Subjects:
Medicine
Genetics
Biotechnology
Cancer
Infectious Diseases
Virology
Computational Biology
system exhibits limitations
successfully identified cases
sera pool dilutions
public health system
cases increasing sharply
borne flavivirus responsible
target ns1 across
reported ns1 concentrations
precision diagnostic tools
3 &# 8211
four denv serotypes
denv genome encodes
structural protein 1
6 &# 8211
early infection detection
ns1 ),
diagnostic sensitivity
detecting ns1
3 %).
denv ),
xlink ">
urgent need
symptom onset
sporadic disease
results underscore
regions facing
recurrent epidemic
recent decades
limited access
key biomarker
human samples
exposing gaps
engineered nanobodies
detection antibodies
detection aligning
detected signals
affecting 18
accurate diagnosis
9 ),
6 %)
23 provinces
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Description
Summary:<p>A-ELISA to detect DENV1-4 NS1 at different concentrations to determine the limit of detection of the optimized cNb-ELISA. The developed detection system has variable LOD for the different DENV NS1. B-Estimation of the analytical sensitivity of the cNb-ELISA, absorbance values obtained based on 2-fold serial dilutions of the pool of positive sera in a range from 1/2 to 1/256. Inset: linear regression analysis of absorbance values based on antigen concentration.</p>

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