Biofilm formation in MRSA strains.

Biofilm formation in MRSA strains.

<p>Quantification of biofilm formation by wild-type (WT), Δ<i>yqhG</i>, and complemented (Δ<i>yqhG</i>-C) strains using crystal violet staining after static incubation in TSB + 1% glucose. Absorbance at 570 nm reflects biofilm biomass. Bars represent the mean ± s.e.m. o...

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Bibliografische gegevens
Hoofdauteur:	Jianhua Liao (1393228) (author)
Andere auteurs:	Jun Cheng (194158) (author), Baoqing Liu (1508821) (author), Yuzhi Shao (22683349) (author), Chunyan Meng (5993228) (author)
Gepubliceerd in:	2025
Onderwerpen:	Medicine Microbiology Cell Biology Biotechnology Evolutionary Biology Ecology Science Policy Infectious Diseases Virology Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified therapeutic strategies aimed novel therapeutic approaches murine infection model div >< p defense mechanisms employed conserved gene encoding compromising membrane stability affect biofilm formation staphylococcus aureus </ reduced bacterial burden improved host survival maintaining mrsa ’ oxidative stress resistance yqhg </ withstand host reduced motility oxidative stress increasing resistance stress adaptation potential target periplasmic protein mrsa virulence mrsa pathogenesis key determinant infected organs increased sensitivity imposed stresses growing prevalence findings highlight existing antibiotics critical need attenuates virulence
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Crystal violet quantification of biofilm formation in MRSA wild-type, ΔyqhG, and complemented strains.
door: Jianhua Liao (1393228)
Gepubliceerd in: (2025)

Spleen bacterial burden in mice infected with MRSA wild-type, ΔyqhG, and complemented strains.
door: Jianhua Liao (1393228)
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Liver bacterial burden in mice infected with MRSA wild-type, ΔyqhG, and complemented strains.
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Kidney bacterial burden in mice infected with MRSA wild-type, Δ<i>yqhG</i>, and complemented strains.
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Heart bacterial burden in mice infected with MRSA wild-type, Δ<i>yqhG</i>, and complemented strains.
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Amount of PSMs (μg/1mL culture) in MRSA wild-type, ΔyqhG, and complemented strains.
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Survival data of BALB/c mice infected with MRSA wild-type, Δ<i>yqhG</i>, and complemented strains.
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Optical density measurements of MRSA wild-type, Δ<i>yqhG</i>, and complemented strains during <i>in vitro</i> growth.
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Primers Used in This Study.
door: Jianhua Liao (1393228)
Gepubliceerd in: (2025)

Bacterial Strains and Plasmids Used in This Study.
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Oxidative stress resistance of the <i>yqhG</i> mutant.
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Quantification of cell-associated PSMs in wild-type, Δ<i>yqhG</i>, and complemented strains. Cell-associated PSMs were extracted from bacterial pellets using 6 M guanidine hydrochloride and quantified by reversed-phase HPLC. The Δ<i>yqhG</i> mutant exhibited a significant reduction in surface-bound PSMs compared with the wild-type strain, whereas complementation restored PSM levels to near wild-type values. Data represent mean ± s.e.m. from three independent experiments. Statistical analysis was performed using one-way ANOVA; *<i>P</i> < 0.05, ns, not significant.
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Surface motility of the <i>yqhG</i> mutant.
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Membrane integrity in the <i>yqhG</i> mutant.
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YqhG is required for systemic virulence but not for <i>in vitro</i> growth.
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Viable CFU counts following hydrogen peroxide exposure.
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Colony diameter measurements from soft agar motility assays.
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SYTOX Green fluorescence measurements for membrane permeability assessment.
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Spearman correlation analysis between gut microbial taxa and circulating metabolites, detailing correlation coefficients, significance values, and taxon–metabolite pairings indicative of microbiome–host metabolic interactions.
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Pathway enrichment analysis of differential metabolites, highlighting significantly impacted metabolic pathways and associated enrichment statistics based on KEGG or other pathway databases.
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