COWP2-mNeon and COWP2-HA localise to the residual body and suture.

COWP2-mNeon and COWP2-HA localise to the residual body and suture.

<p>A) COWP2-mNeon oocysts hatched and stained with primary and secondary antibodies. Hatched oocysts in cyan (1E3-AlexaFluor-647), HA in magenta (anti-HA-AlexaFluor-546), mNeon in green (epifluorescence). B) COWP2-mNeon oocysts hatched and stained only with secondary antibodies AlexaFluor-546...

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Bibliographic Details
Main Author: Ross Bacchetti (16752855) (author)
Other Authors: Sarah Stevens (398830) (author), Leandro Lemgruber (2882552) (author), Mariana Azevedo Gonzalez Oliva (22486492) (author), Emma M. Sands (16752861) (author), Konstantinos Alexandrou (22486495) (author), Michele Tinti (75217) (author), Lee Robinson (9651716) (author), Jack C. Hanna (16752846) (author), Simona Seizova (16752852) (author), Peyton Goddard (22486498) (author), Massimo Vassalli (2178036) (author), Mattie Christine Pawlowic (22486501) (author)
Published: 2025
Subjects:
Biochemistry
Microbiology
Cell Biology
Genetics
Ecology
Immunology
Infectious Diseases
Computational Biology
Space Science
Environmental Sciences not elsewhere classified
Physical Sciences not elsewhere classified
diarrhoeal disease contributing
biomechanical measurements determine
2 – 9
common water treatments
typical oocyst morphology
oocyst wall formation
hardy oocyst wall
produce viable oocysts
div >< p
oocyst wall proteins
oocyst wall
effective treatments
oocyst suture
young children
work confirms
using crispr
true components
technical challenges
substantial morbidity
significant cause
secrete material
resists chlorination
remaining members
puncta consistent
organelles store
often waterborne
microscopy confirms
knockout oocysts
including chlorination
fluorescent fusions
cryptosporidium </
cowp8 </
cowp1 </
cowp </
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Summary:<p>A) COWP2-mNeon oocysts hatched and stained with primary and secondary antibodies. Hatched oocysts in cyan (1E3-AlexaFluor-647), HA in magenta (anti-HA-AlexaFluor-546), mNeon in green (epifluorescence). B) COWP2-mNeon oocysts hatched and stained only with secondary antibodies AlexaFluor-546 and AlexaFluor-647. C) COWP2-HA oocysts hatched and stained with primary and secondary antibodies. D) COWP2-HA oocysts hatched and stained only with secondary antibodies AlexaFluor-546 and AlexaFluor-647. Images collected on a Zeiss LSM880 Airyscan microscope, confocal mode. Scale bar for all images is 10 µm. Contrast adjustments are the same for all images which are a representative single z-plane. Image collection parameters are the same for each image exposure time 0.01 msec and laser settings: Alexa-647 laser 0.5%; Alexa-546 laser 2%; mNeonGreen laser 2.3%.</p> <p>(TIF)</p>

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