COWP2-mNeon and COWP2-HA localise to the residual body and suture.
<p>A) COWP2-mNeon oocysts hatched and stained with primary and secondary antibodies. Hatched oocysts in cyan (1E3-AlexaFluor-647), HA in magenta (anti-HA-AlexaFluor-546), mNeon in green (epifluorescence). B) COWP2-mNeon oocysts hatched and stained only with secondary antibodies AlexaFluor-546...
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2025
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| _version_ | 1852015577878495232 |
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| author | Ross Bacchetti (16752855) |
| author2 | Sarah Stevens (398830) Leandro Lemgruber (2882552) Mariana Azevedo Gonzalez Oliva (22486492) Emma M. Sands (16752861) Konstantinos Alexandrou (22486495) Michele Tinti (75217) Lee Robinson (9651716) Jack C. Hanna (16752846) Simona Seizova (16752852) Peyton Goddard (22486498) Massimo Vassalli (2178036) Mattie Christine Pawlowic (22486501) |
| author2_role | author author author author author author author author author author author author |
| author_facet | Ross Bacchetti (16752855) Sarah Stevens (398830) Leandro Lemgruber (2882552) Mariana Azevedo Gonzalez Oliva (22486492) Emma M. Sands (16752861) Konstantinos Alexandrou (22486495) Michele Tinti (75217) Lee Robinson (9651716) Jack C. Hanna (16752846) Simona Seizova (16752852) Peyton Goddard (22486498) Massimo Vassalli (2178036) Mattie Christine Pawlowic (22486501) |
| author_role | author |
| dc.creator.none.fl_str_mv | Ross Bacchetti (16752855) Sarah Stevens (398830) Leandro Lemgruber (2882552) Mariana Azevedo Gonzalez Oliva (22486492) Emma M. Sands (16752861) Konstantinos Alexandrou (22486495) Michele Tinti (75217) Lee Robinson (9651716) Jack C. Hanna (16752846) Simona Seizova (16752852) Peyton Goddard (22486498) Massimo Vassalli (2178036) Mattie Christine Pawlowic (22486501) |
| dc.date.none.fl_str_mv | 2025-10-23T17:39:09Z |
| dc.identifier.none.fl_str_mv | 10.1371/journal.ppat.1013561.s008 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/figure/COWP2-mNeon_and_COWP2-HA_localise_to_the_residual_body_and_suture_/30431263 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biochemistry Microbiology Cell Biology Genetics Ecology Immunology Infectious Diseases Computational Biology Space Science Environmental Sciences not elsewhere classified Physical Sciences not elsewhere classified diarrhoeal disease contributing biomechanical measurements determine 2 – 9 common water treatments typical oocyst morphology oocyst wall formation hardy oocyst wall produce viable oocysts div >< p oocyst wall proteins oocyst wall effective treatments oocyst suture young children work confirms using crispr true components technical challenges substantial morbidity significant cause secrete material resists chlorination remaining members puncta consistent organelles store often waterborne microscopy confirms knockout oocysts including chlorination fluorescent fusions cryptosporidium </ cowp8 </ cowp1 </ cowp </ |
| dc.title.none.fl_str_mv | COWP2-mNeon and COWP2-HA localise to the residual body and suture. |
| dc.type.none.fl_str_mv | Image Figure info:eu-repo/semantics/publishedVersion image |
| description | <p>A) COWP2-mNeon oocysts hatched and stained with primary and secondary antibodies. Hatched oocysts in cyan (1E3-AlexaFluor-647), HA in magenta (anti-HA-AlexaFluor-546), mNeon in green (epifluorescence). B) COWP2-mNeon oocysts hatched and stained only with secondary antibodies AlexaFluor-546 and AlexaFluor-647. C) COWP2-HA oocysts hatched and stained with primary and secondary antibodies. D) COWP2-HA oocysts hatched and stained only with secondary antibodies AlexaFluor-546 and AlexaFluor-647. Images collected on a Zeiss LSM880 Airyscan microscope, confocal mode. Scale bar for all images is 10 µm. Contrast adjustments are the same for all images which are a representative single z-plane. Image collection parameters are the same for each image exposure time 0.01 msec and laser settings: Alexa-647 laser 0.5%; Alexa-546 laser 2%; mNeonGreen laser 2.3%.</p> <p>(TIF)</p> |
| eu_rights_str_mv | openAccess |
| id | Manara_98d08d2b63518b59b646bdc588bc2e2a |
| identifier_str_mv | 10.1371/journal.ppat.1013561.s008 |
| network_acronym_str | Manara |
| network_name_str | ManaraRepo |
| oai_identifier_str | oai:figshare.com:article/30431263 |
| publishDate | 2025 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | COWP2-mNeon and COWP2-HA localise to the residual body and suture.Ross Bacchetti (16752855)Sarah Stevens (398830)Leandro Lemgruber (2882552)Mariana Azevedo Gonzalez Oliva (22486492)Emma M. Sands (16752861)Konstantinos Alexandrou (22486495)Michele Tinti (75217)Lee Robinson (9651716)Jack C. Hanna (16752846)Simona Seizova (16752852)Peyton Goddard (22486498)Massimo Vassalli (2178036)Mattie Christine Pawlowic (22486501)BiochemistryMicrobiologyCell BiologyGeneticsEcologyImmunologyInfectious DiseasesComputational BiologySpace ScienceEnvironmental Sciences not elsewhere classifiedPhysical Sciences not elsewhere classifieddiarrhoeal disease contributingbiomechanical measurements determine2 – 9common water treatmentstypical oocyst morphologyoocyst wall formationhardy oocyst wallproduce viable oocystsdiv >< poocyst wall proteinsoocyst walleffective treatmentsoocyst sutureyoung childrenwork confirmsusing crisprtrue componentstechnical challengessubstantial morbiditysignificant causesecrete materialresists chlorinationremaining memberspuncta consistentorganelles storeoften waterbornemicroscopy confirmsknockout oocystsincluding chlorinationfluorescent fusionscryptosporidium </cowp8 </cowp1 </cowp </<p>A) COWP2-mNeon oocysts hatched and stained with primary and secondary antibodies. Hatched oocysts in cyan (1E3-AlexaFluor-647), HA in magenta (anti-HA-AlexaFluor-546), mNeon in green (epifluorescence). B) COWP2-mNeon oocysts hatched and stained only with secondary antibodies AlexaFluor-546 and AlexaFluor-647. C) COWP2-HA oocysts hatched and stained with primary and secondary antibodies. D) COWP2-HA oocysts hatched and stained only with secondary antibodies AlexaFluor-546 and AlexaFluor-647. Images collected on a Zeiss LSM880 Airyscan microscope, confocal mode. Scale bar for all images is 10 µm. Contrast adjustments are the same for all images which are a representative single z-plane. Image collection parameters are the same for each image exposure time 0.01 msec and laser settings: Alexa-647 laser 0.5%; Alexa-546 laser 2%; mNeonGreen laser 2.3%.</p> <p>(TIF)</p>2025-10-23T17:39:09ZImageFigureinfo:eu-repo/semantics/publishedVersionimage10.1371/journal.ppat.1013561.s008https://figshare.com/articles/figure/COWP2-mNeon_and_COWP2-HA_localise_to_the_residual_body_and_suture_/30431263CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/304312632025-10-23T17:39:09Z |
| spellingShingle | COWP2-mNeon and COWP2-HA localise to the residual body and suture. Ross Bacchetti (16752855) Biochemistry Microbiology Cell Biology Genetics Ecology Immunology Infectious Diseases Computational Biology Space Science Environmental Sciences not elsewhere classified Physical Sciences not elsewhere classified diarrhoeal disease contributing biomechanical measurements determine 2 – 9 common water treatments typical oocyst morphology oocyst wall formation hardy oocyst wall produce viable oocysts div >< p oocyst wall proteins oocyst wall effective treatments oocyst suture young children work confirms using crispr true components technical challenges substantial morbidity significant cause secrete material resists chlorination remaining members puncta consistent organelles store often waterborne microscopy confirms knockout oocysts including chlorination fluorescent fusions cryptosporidium </ cowp8 </ cowp1 </ cowp </ |
| status_str | publishedVersion |
| title | COWP2-mNeon and COWP2-HA localise to the residual body and suture. |
| title_full | COWP2-mNeon and COWP2-HA localise to the residual body and suture. |
| title_fullStr | COWP2-mNeon and COWP2-HA localise to the residual body and suture. |
| title_full_unstemmed | COWP2-mNeon and COWP2-HA localise to the residual body and suture. |
| title_short | COWP2-mNeon and COWP2-HA localise to the residual body and suture. |
| title_sort | COWP2-mNeon and COWP2-HA localise to the residual body and suture. |
| topic | Biochemistry Microbiology Cell Biology Genetics Ecology Immunology Infectious Diseases Computational Biology Space Science Environmental Sciences not elsewhere classified Physical Sciences not elsewhere classified diarrhoeal disease contributing biomechanical measurements determine 2 – 9 common water treatments typical oocyst morphology oocyst wall formation hardy oocyst wall produce viable oocysts div >< p oocyst wall proteins oocyst wall effective treatments oocyst suture young children work confirms using crispr true components technical challenges substantial morbidity significant cause secrete material resists chlorination remaining members puncta consistent organelles store often waterborne microscopy confirms knockout oocysts including chlorination fluorescent fusions cryptosporidium </ cowp8 </ cowp1 </ cowp </ |