All Data Supporting the Findings of This Study.
<div><p>Rotavirus A (RVA) spreads to multiple extraintestinal organs; however, it is not well understood what viral or host characteristics regulate the efficiency of this spread. We conducted this study to determine whether more efficient intestinal RVA replication leads to a higher rat...
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2025
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| _version_ | 1849927626875994112 |
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| author | Sergei A. Raev (15350287) |
| author2 | Maryssa K. Kick (17183901) Maria Chellis (22683628) Linda J. Saif (6626666) Talita P. Resende (3916268) Anastasia N. Vlasova (6626663) |
| author2_role | author author author author author |
| author_facet | Sergei A. Raev (15350287) Maryssa K. Kick (17183901) Maria Chellis (22683628) Linda J. Saif (6626666) Talita P. Resende (3916268) Anastasia N. Vlasova (6626663) |
| author_role | author |
| dc.creator.none.fl_str_mv | Sergei A. Raev (15350287) Maryssa K. Kick (17183901) Maria Chellis (22683628) Linda J. Saif (6626666) Talita P. Resende (3916268) Anastasia N. Vlasova (6626663) |
| dc.date.none.fl_str_mv | 2025-11-25T18:38:48Z |
| dc.identifier.none.fl_str_mv | 10.1371/journal.ppat.1013723.s009 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/dataset/All_Data_Supporting_the_Findings_of_This_Study_/30714691 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biophysics Biochemistry Microbiology Ecology Infectious Diseases Space Science Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified increased sialic acid host characteristics regulate first experimental evidence findings may provide current global dominance rva rna detection multiple extraintestinal organs div >< p rva strains replicated related rva strains porcine osu g5p facilitating viral dissemination efficient extraintestinal spread rva receptor diversity rva extraintestinal spread examined extraintestinal tissues gut </ p extraintestinal tissues extraintestinal spread extraintestinal tissue extraintestinal sites also examined vivo </ vitro </ different tissues well understood respiratory lesions remarkably robust potential role pigs infected moderate titers microscopic lesions intestinal tissue inoculated germ highest titers highest frequency higher rate free pigs determine whether better understand |
| dc.title.none.fl_str_mv | All Data Supporting the Findings of This Study. |
| dc.type.none.fl_str_mv | Dataset info:eu-repo/semantics/publishedVersion dataset |
| description | <div><p>Rotavirus A (RVA) spreads to multiple extraintestinal organs; however, it is not well understood what viral or host characteristics regulate the efficiency of this spread. We conducted this study to determine whether more efficient intestinal RVA replication leads to a higher rate of its extraintestinal spread. We also examined the distribution of known RVA glycan receptors in different tissues to better understand their potential role in facilitating viral dissemination to extraintestinal sites. We inoculated germ-free pigs with porcine OSU G5P[7] characterized by remarkably robust <i>in vitro</i>/<i>in vivo</i> replication, and G9P[13] which replicates to low-to-moderate titers and several other strains. Significantly higher RVA titers were observed in intestinal tissue/contents/feces of pigs infected with G5P[7], whereas G9P[13] was associated with a relatively modest intestinal replication but the most efficient extraintestinal spread. As expected, and coinciding with the increased sialic acid/glycan abundance and diversity, all RVA strains replicated to highest titers in the gut. Further, among the examined extraintestinal tissues, the lungs: a) had the highest frequency of RVA RNA detection; b) exhibited the highest host glycan diversity/abundance; and c) represented the only extraintestinal tissue in which both gross and microscopic lesions were observed. This further underscores the association between the RVA receptor diversity and respiratory lesions. This is the first experimental evidence that RVA extraintestinal spread does not depend on its replication efficiency in the gut. Additionally, these findings may provide an explanation for the current global dominance of G9P[13] and related RVA strains, which could be capable of airborne spread.</p></div> |
| eu_rights_str_mv | openAccess |
| id | Manara_9c86db2fd565f9d3d6929a51e0d7063c |
| identifier_str_mv | 10.1371/journal.ppat.1013723.s009 |
| network_acronym_str | Manara |
| network_name_str | ManaraRepo |
| oai_identifier_str | oai:figshare.com:article/30714691 |
| publishDate | 2025 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | All Data Supporting the Findings of This Study.Sergei A. Raev (15350287)Maryssa K. Kick (17183901)Maria Chellis (22683628)Linda J. Saif (6626666)Talita P. Resende (3916268)Anastasia N. Vlasova (6626663)BiophysicsBiochemistryMicrobiologyEcologyInfectious DiseasesSpace ScienceEnvironmental Sciences not elsewhere classifiedBiological Sciences not elsewhere classifiedincreased sialic acidhost characteristics regulatefirst experimental evidencefindings may providecurrent global dominancerva rna detectionmultiple extraintestinal organsdiv >< prva strains replicatedrelated rva strainsporcine osu g5pfacilitating viral disseminationefficient extraintestinal spreadrva receptor diversityrva extraintestinal spreadexamined extraintestinal tissuesgut </ pextraintestinal tissuesextraintestinal spreadextraintestinal tissueextraintestinal sitesalso examinedvivo </vitro </different tissueswell understoodrespiratory lesionsremarkably robustpotential rolepigs infectedmoderate titersmicroscopic lesionsintestinal tissueinoculated germhighest titershighest frequencyhigher ratefree pigsdetermine whetherbetter understand<div><p>Rotavirus A (RVA) spreads to multiple extraintestinal organs; however, it is not well understood what viral or host characteristics regulate the efficiency of this spread. We conducted this study to determine whether more efficient intestinal RVA replication leads to a higher rate of its extraintestinal spread. We also examined the distribution of known RVA glycan receptors in different tissues to better understand their potential role in facilitating viral dissemination to extraintestinal sites. We inoculated germ-free pigs with porcine OSU G5P[7] characterized by remarkably robust <i>in vitro</i>/<i>in vivo</i> replication, and G9P[13] which replicates to low-to-moderate titers and several other strains. Significantly higher RVA titers were observed in intestinal tissue/contents/feces of pigs infected with G5P[7], whereas G9P[13] was associated with a relatively modest intestinal replication but the most efficient extraintestinal spread. As expected, and coinciding with the increased sialic acid/glycan abundance and diversity, all RVA strains replicated to highest titers in the gut. Further, among the examined extraintestinal tissues, the lungs: a) had the highest frequency of RVA RNA detection; b) exhibited the highest host glycan diversity/abundance; and c) represented the only extraintestinal tissue in which both gross and microscopic lesions were observed. This further underscores the association between the RVA receptor diversity and respiratory lesions. This is the first experimental evidence that RVA extraintestinal spread does not depend on its replication efficiency in the gut. Additionally, these findings may provide an explanation for the current global dominance of G9P[13] and related RVA strains, which could be capable of airborne spread.</p></div>2025-11-25T18:38:48ZDatasetinfo:eu-repo/semantics/publishedVersiondataset10.1371/journal.ppat.1013723.s009https://figshare.com/articles/dataset/All_Data_Supporting_the_Findings_of_This_Study_/30714691CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/307146912025-11-25T18:38:48Z |
| spellingShingle | All Data Supporting the Findings of This Study. Sergei A. Raev (15350287) Biophysics Biochemistry Microbiology Ecology Infectious Diseases Space Science Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified increased sialic acid host characteristics regulate first experimental evidence findings may provide current global dominance rva rna detection multiple extraintestinal organs div >< p rva strains replicated related rva strains porcine osu g5p facilitating viral dissemination efficient extraintestinal spread rva receptor diversity rva extraintestinal spread examined extraintestinal tissues gut </ p extraintestinal tissues extraintestinal spread extraintestinal tissue extraintestinal sites also examined vivo </ vitro </ different tissues well understood respiratory lesions remarkably robust potential role pigs infected moderate titers microscopic lesions intestinal tissue inoculated germ highest titers highest frequency higher rate free pigs determine whether better understand |
| status_str | publishedVersion |
| title | All Data Supporting the Findings of This Study. |
| title_full | All Data Supporting the Findings of This Study. |
| title_fullStr | All Data Supporting the Findings of This Study. |
| title_full_unstemmed | All Data Supporting the Findings of This Study. |
| title_short | All Data Supporting the Findings of This Study. |
| title_sort | All Data Supporting the Findings of This Study. |
| topic | Biophysics Biochemistry Microbiology Ecology Infectious Diseases Space Science Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified increased sialic acid host characteristics regulate first experimental evidence findings may provide current global dominance rva rna detection multiple extraintestinal organs div >< p rva strains replicated related rva strains porcine osu g5p facilitating viral dissemination efficient extraintestinal spread rva receptor diversity rva extraintestinal spread examined extraintestinal tissues gut </ p extraintestinal tissues extraintestinal spread extraintestinal tissue extraintestinal sites also examined vivo </ vitro </ different tissues well understood respiratory lesions remarkably robust potential role pigs infected moderate titers microscopic lesions intestinal tissue inoculated germ highest titers highest frequency higher rate free pigs determine whether better understand |