Expression of <i>Lac2</i> mRNA.
<p>(A) Temporal expression pattern of the <i>Lac2</i> transcript in early egg development, as analyzed using quantitative RT-PCR. The expression levels (Y-axis) are presented as values normalized to the house-keeping gene <i>rp49</i>. Data are plotted as means (black ci...
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2025
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| 要約: | <p>(A) Temporal expression pattern of the <i>Lac2</i> transcript in early egg development, as analyzed using quantitative RT-PCR. The expression levels (Y-axis) are presented as values normalized to the house-keeping gene <i>rp49</i>. Data are plotted as means (black circles) and standard deviation (black lines). Individual data points are plotted as orange dots. The accompanying images depict eggs at five developmental stages (<24 h, 30– 40 h, 40– 50 h, 50– 60 h, and >70 h after AEL (after egg laying), illustrating the progressive pigmentation of the eggs. (B) <i>Lac2</i> transcript expression levels across various morphs and developmental stages, as analyzed using qRT-PCR. On the x-axis, “early” represents 0–72 h after egg laying, corresponding to data in (A); “middle” represents 1-week AEL; and “late” represents eggs at 1.5–2.0 months of AEL following diapause. “Fundatrix L1” indicates the L1 larva that hatches from overwintering eggs. For viviparous females, samples are further categorized as nymphs (L1–L4 stages mixed), molting, adult, and winged morphs. Winged viviparous morphs were induced under crowded conditions. In all cases, whole bodies or whole eggs were homogenized for qRT-PCR quantification. Expression levels are normalized to the housekeeping gene <i>rp49</i>.</p> |
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