Filamin A KO and S2152A KI reduce KSHV de novo infection of cell-free virions.
<p>A-B. Filamin WT, S2152A KI or KO HEK293-mCherry cells were transfected with empty vector or ORF45 expressing plasmid for 24 h (A), or left uninfected or infected with Bac16 or STOP45 viral stocks (MOI = 10) for 24 h (B). The cells were collected and whole cell extracted were subjected to We...
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2025
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| _version_ | 1849927641315934208 |
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| author | Xiaojuan Li (351842) |
| author2 | Li Quan (177024) Rihong Zhou (22676690) Xiangpeng Liu (17898881) Ronit Sarid (186795) Ersheng Kuang (129028) |
| author2_role | author author author author author |
| author_facet | Xiaojuan Li (351842) Li Quan (177024) Rihong Zhou (22676690) Xiangpeng Liu (17898881) Ronit Sarid (186795) Ersheng Kuang (129028) |
| author_role | author |
| dc.creator.none.fl_str_mv | Xiaojuan Li (351842) Li Quan (177024) Rihong Zhou (22676690) Xiangpeng Liu (17898881) Ronit Sarid (186795) Ersheng Kuang (129028) |
| dc.date.none.fl_str_mv | 2025-11-24T18:34:26Z |
| dc.identifier.none.fl_str_mv | 10.1371/journal.ppat.1013737.g005 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/figure/Filamin_A_KO_and_S2152A_KI_reduce_KSHV_de_novo_infection_of_cell-free_virions_/30697706 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Microbiology Cell Biology Molecular Biology Biotechnology Immunology Developmental Biology Cancer Infectious Diseases Virology s2152a knockin abolish primary effusion lymphoma multicentric castleman disease kaposi &# 8217 mediated viral infection infected adherent cells differentiated b cells sequential cell detachment mediated kshv transmission kshv lytic replication adherent cells lytic replication viral transmission kshv transmission cell detachment b lymphocytes lytic cycle cell transmission taken together results demonstrated radically support natural donors key role key regulator etiological agent dependent manner cell contact cell adhesion associated herpesvirus |
| dc.title.none.fl_str_mv | Filamin A KO and S2152A KI reduce KSHV de novo infection of cell-free virions. |
| dc.type.none.fl_str_mv | Image Figure info:eu-repo/semantics/publishedVersion image |
| description | <p>A-B. Filamin WT, S2152A KI or KO HEK293-mCherry cells were transfected with empty vector or ORF45 expressing plasmid for 24 h (A), or left uninfected or infected with Bac16 or STOP45 viral stocks (MOI = 10) for 24 h (B). The cells were collected and whole cell extracted were subjected to Western blotting analysis as indicated. C-E. Filamin WT, S2152A KI or KO HEK293-mCherry cells were left uninfected or infected with iSLK.Bac16 or iSLK.STOP45 viral stocks (MOI = 10). (C) Two hours post infection, the cells were collected by trypsin digestion, and the intracellular viral DNA and cellular DNA genomes were extracted. (D) Eight hours post infection, the cells were collected and nuclear fractions were isolated, and then viral DNA and cellular DNA genome were extracted from nuclear fractions. The viral DNA copy numbers were detected by real-time PCR and GAPDH DNA was used as internal control. (E) Twenty four hours post infection, the total RNA were extracted, reverse-transcribed and detected by real-time PCR. The levels of viral mRNA were normalized to β-actin mRNA.. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001, t test.</p> |
| eu_rights_str_mv | openAccess |
| id | Manara_9cf715721b995d6594306ad231f62a1b |
| identifier_str_mv | 10.1371/journal.ppat.1013737.g005 |
| network_acronym_str | Manara |
| network_name_str | ManaraRepo |
| oai_identifier_str | oai:figshare.com:article/30697706 |
| publishDate | 2025 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Filamin A KO and S2152A KI reduce KSHV de novo infection of cell-free virions.Xiaojuan Li (351842)Li Quan (177024)Rihong Zhou (22676690)Xiangpeng Liu (17898881)Ronit Sarid (186795)Ersheng Kuang (129028)MicrobiologyCell BiologyMolecular BiologyBiotechnologyImmunologyDevelopmental BiologyCancerInfectious DiseasesVirologys2152a knockin abolishprimary effusion lymphomamulticentric castleman diseasekaposi &# 8217mediated viral infectioninfected adherent cellsdifferentiated b cellssequential cell detachmentmediated kshv transmissionkshv lytic replicationadherent cellslytic replicationviral transmissionkshv transmissioncell detachmentb lymphocyteslytic cyclecell transmissiontaken togetherresults demonstratedradically supportnatural donorskey rolekey regulatoretiological agentdependent mannercell contactcell adhesionassociated herpesvirus<p>A-B. Filamin WT, S2152A KI or KO HEK293-mCherry cells were transfected with empty vector or ORF45 expressing plasmid for 24 h (A), or left uninfected or infected with Bac16 or STOP45 viral stocks (MOI = 10) for 24 h (B). The cells were collected and whole cell extracted were subjected to Western blotting analysis as indicated. C-E. Filamin WT, S2152A KI or KO HEK293-mCherry cells were left uninfected or infected with iSLK.Bac16 or iSLK.STOP45 viral stocks (MOI = 10). (C) Two hours post infection, the cells were collected by trypsin digestion, and the intracellular viral DNA and cellular DNA genomes were extracted. (D) Eight hours post infection, the cells were collected and nuclear fractions were isolated, and then viral DNA and cellular DNA genome were extracted from nuclear fractions. The viral DNA copy numbers were detected by real-time PCR and GAPDH DNA was used as internal control. (E) Twenty four hours post infection, the total RNA were extracted, reverse-transcribed and detected by real-time PCR. The levels of viral mRNA were normalized to β-actin mRNA.. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001, t test.</p>2025-11-24T18:34:26ZImageFigureinfo:eu-repo/semantics/publishedVersionimage10.1371/journal.ppat.1013737.g005https://figshare.com/articles/figure/Filamin_A_KO_and_S2152A_KI_reduce_KSHV_de_novo_infection_of_cell-free_virions_/30697706CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/306977062025-11-24T18:34:26Z |
| spellingShingle | Filamin A KO and S2152A KI reduce KSHV de novo infection of cell-free virions. Xiaojuan Li (351842) Microbiology Cell Biology Molecular Biology Biotechnology Immunology Developmental Biology Cancer Infectious Diseases Virology s2152a knockin abolish primary effusion lymphoma multicentric castleman disease kaposi &# 8217 mediated viral infection infected adherent cells differentiated b cells sequential cell detachment mediated kshv transmission kshv lytic replication adherent cells lytic replication viral transmission kshv transmission cell detachment b lymphocytes lytic cycle cell transmission taken together results demonstrated radically support natural donors key role key regulator etiological agent dependent manner cell contact cell adhesion associated herpesvirus |
| status_str | publishedVersion |
| title | Filamin A KO and S2152A KI reduce KSHV de novo infection of cell-free virions. |
| title_full | Filamin A KO and S2152A KI reduce KSHV de novo infection of cell-free virions. |
| title_fullStr | Filamin A KO and S2152A KI reduce KSHV de novo infection of cell-free virions. |
| title_full_unstemmed | Filamin A KO and S2152A KI reduce KSHV de novo infection of cell-free virions. |
| title_short | Filamin A KO and S2152A KI reduce KSHV de novo infection of cell-free virions. |
| title_sort | Filamin A KO and S2152A KI reduce KSHV de novo infection of cell-free virions. |
| topic | Microbiology Cell Biology Molecular Biology Biotechnology Immunology Developmental Biology Cancer Infectious Diseases Virology s2152a knockin abolish primary effusion lymphoma multicentric castleman disease kaposi &# 8217 mediated viral infection infected adherent cells differentiated b cells sequential cell detachment mediated kshv transmission kshv lytic replication adherent cells lytic replication viral transmission kshv transmission cell detachment b lymphocytes lytic cycle cell transmission taken together results demonstrated radically support natural donors key role key regulator etiological agent dependent manner cell contact cell adhesion associated herpesvirus |