Table 2_Mangiferin mitigates neurological deficits and ferroptosis via NRF2/ARE pathway activation in cerebral ischemia-reperfusion rats.xlsx
Introduction<p>Ferroptosis is a newly described form of nonapoptotic, iron-dependent cell death that plays an essential role in the pathogenesis of ischemic stroke. Targeting ferroptosis may be an effective way to treat ischemic stroke. Mangiferin (MGF) is a natural polyphenol that has been sh...
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2025
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| _version_ | 1852020153146933248 |
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| author | Chenjia Peng (21404753) |
| author2 | Yang Zhang (30734) Jiaqing Chai (21404756) Hengbo Zhang (6458453) |
| author2_role | author author author |
| author_facet | Chenjia Peng (21404753) Yang Zhang (30734) Jiaqing Chai (21404756) Hengbo Zhang (6458453) |
| author_role | author |
| dc.creator.none.fl_str_mv | Chenjia Peng (21404753) Yang Zhang (30734) Jiaqing Chai (21404756) Hengbo Zhang (6458453) |
| dc.date.none.fl_str_mv | 2025-05-22T05:22:38Z |
| dc.identifier.none.fl_str_mv | 10.3389/fphar.2025.1577954.s002 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/dataset/Table_2_Mangiferin_mitigates_neurological_deficits_and_ferroptosis_via_NRF2_ARE_pathway_activation_in_cerebral_ischemia-reperfusion_rats_xlsx/29124821 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Pharmacology MGF ischemic stroke ferroptosis Nrf2/ARE pathway neuroprotection |
| dc.title.none.fl_str_mv | Table 2_Mangiferin mitigates neurological deficits and ferroptosis via NRF2/ARE pathway activation in cerebral ischemia-reperfusion rats.xlsx |
| dc.type.none.fl_str_mv | Dataset info:eu-repo/semantics/publishedVersion dataset |
| description | Introduction<p>Ferroptosis is a newly described form of nonapoptotic, iron-dependent cell death that plays an essential role in the pathogenesis of ischemic stroke. Targeting ferroptosis may be an effective way to treat ischemic stroke. Mangiferin (MGF) is a natural polyphenol that has been shown to protect neurological function via multiple mechanisms. However, the mechanism by which MGF inhibits ferroptosis in stroke remains unclear.</p>Methods<p>An ischemic stroke rat model was established by middle cerebral artery occlusion. Neurological scoring, TTC staining, behavioral tests, Nissl staining, HE staining and immunochemistry were used to analyze the influences of MGF on neurological deficits, the infarct area, cognitive function, neuronal morphology, histopathological injury, and the morphology of microglia and astrocytes. Transmission electron microscopy and Perls’ stain were used to evaluate the characteristics of ferroptosis. Western blotting was used to analyze the expression of NRF2, FTL, SLC7A11 and GPX4. ELISA was used to analyze the levels of cytokines, including IL-6, IL-1β and TNF-α, to evaluate neuroinflammation. Oxidative stress was evaluated by analyzing the levels of ROS, MDA, GSH, and SOD.</p>Results<p>MGF clearly improved the neurological function and learning and memory ability of stroke rats. MGF significantly decreased ROS and MDA and increased GSH, SOD. MGF significantly suppressed neuroinflammation by downregulating IL-6, IL-1β and TNF-α. Ferroptosis in stroke rats was significantly inhibited by MGF. MGF significantly increased the expression of NRF2, FTL, SLC7A11 and GPX4. The NRF2 inhibitor ML385 significantly reversed the effects of MGF on stroke rats.</p>Conclusion<p>MGF protects neurological function and suppresses ferroptosis via activating NRF2/ARE pathway in ischemic stroke rats.</p> |
| eu_rights_str_mv | openAccess |
| id | Manara_a3b324fdcf0bca07f083ea9be4977f1a |
| identifier_str_mv | 10.3389/fphar.2025.1577954.s002 |
| network_acronym_str | Manara |
| network_name_str | ManaraRepo |
| oai_identifier_str | oai:figshare.com:article/29124821 |
| publishDate | 2025 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Table 2_Mangiferin mitigates neurological deficits and ferroptosis via NRF2/ARE pathway activation in cerebral ischemia-reperfusion rats.xlsxChenjia Peng (21404753)Yang Zhang (30734)Jiaqing Chai (21404756)Hengbo Zhang (6458453)PharmacologyMGFischemic strokeferroptosisNrf2/ARE pathwayneuroprotectionIntroduction<p>Ferroptosis is a newly described form of nonapoptotic, iron-dependent cell death that plays an essential role in the pathogenesis of ischemic stroke. Targeting ferroptosis may be an effective way to treat ischemic stroke. Mangiferin (MGF) is a natural polyphenol that has been shown to protect neurological function via multiple mechanisms. However, the mechanism by which MGF inhibits ferroptosis in stroke remains unclear.</p>Methods<p>An ischemic stroke rat model was established by middle cerebral artery occlusion. Neurological scoring, TTC staining, behavioral tests, Nissl staining, HE staining and immunochemistry were used to analyze the influences of MGF on neurological deficits, the infarct area, cognitive function, neuronal morphology, histopathological injury, and the morphology of microglia and astrocytes. Transmission electron microscopy and Perls’ stain were used to evaluate the characteristics of ferroptosis. Western blotting was used to analyze the expression of NRF2, FTL, SLC7A11 and GPX4. ELISA was used to analyze the levels of cytokines, including IL-6, IL-1β and TNF-α, to evaluate neuroinflammation. Oxidative stress was evaluated by analyzing the levels of ROS, MDA, GSH, and SOD.</p>Results<p>MGF clearly improved the neurological function and learning and memory ability of stroke rats. MGF significantly decreased ROS and MDA and increased GSH, SOD. MGF significantly suppressed neuroinflammation by downregulating IL-6, IL-1β and TNF-α. Ferroptosis in stroke rats was significantly inhibited by MGF. MGF significantly increased the expression of NRF2, FTL, SLC7A11 and GPX4. The NRF2 inhibitor ML385 significantly reversed the effects of MGF on stroke rats.</p>Conclusion<p>MGF protects neurological function and suppresses ferroptosis via activating NRF2/ARE pathway in ischemic stroke rats.</p>2025-05-22T05:22:38ZDatasetinfo:eu-repo/semantics/publishedVersiondataset10.3389/fphar.2025.1577954.s002https://figshare.com/articles/dataset/Table_2_Mangiferin_mitigates_neurological_deficits_and_ferroptosis_via_NRF2_ARE_pathway_activation_in_cerebral_ischemia-reperfusion_rats_xlsx/29124821CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/291248212025-05-22T05:22:38Z |
| spellingShingle | Table 2_Mangiferin mitigates neurological deficits and ferroptosis via NRF2/ARE pathway activation in cerebral ischemia-reperfusion rats.xlsx Chenjia Peng (21404753) Pharmacology MGF ischemic stroke ferroptosis Nrf2/ARE pathway neuroprotection |
| status_str | publishedVersion |
| title | Table 2_Mangiferin mitigates neurological deficits and ferroptosis via NRF2/ARE pathway activation in cerebral ischemia-reperfusion rats.xlsx |
| title_full | Table 2_Mangiferin mitigates neurological deficits and ferroptosis via NRF2/ARE pathway activation in cerebral ischemia-reperfusion rats.xlsx |
| title_fullStr | Table 2_Mangiferin mitigates neurological deficits and ferroptosis via NRF2/ARE pathway activation in cerebral ischemia-reperfusion rats.xlsx |
| title_full_unstemmed | Table 2_Mangiferin mitigates neurological deficits and ferroptosis via NRF2/ARE pathway activation in cerebral ischemia-reperfusion rats.xlsx |
| title_short | Table 2_Mangiferin mitigates neurological deficits and ferroptosis via NRF2/ARE pathway activation in cerebral ischemia-reperfusion rats.xlsx |
| title_sort | Table 2_Mangiferin mitigates neurological deficits and ferroptosis via NRF2/ARE pathway activation in cerebral ischemia-reperfusion rats.xlsx |
| topic | Pharmacology MGF ischemic stroke ferroptosis Nrf2/ARE pathway neuroprotection |