Verification of RNAi knockdowns.

Verification of RNAi knockdowns.

<p>The knockdowns of (A) SIK3, (B) XRCC1, (C) TFDP2 and (D) PRIM1 in 293T cells were verified by western blots at 24 hours.</p> <p>(TIFF)</p>

Gorde:

Xehetasun bibliografikoak
Egile nagusia:	Monnette F. Summers (22075935) (author)
Beste egile batzuk:	MegAnn K. Haubold (15990697) (author), Marcel Morgenstern (5679425) (author), Phoenix Shepherd (22075938) (author), Clairine I. S. Larsen (15990709) (author), Ava E. Bartz (22075941) (author), Gopishankar Thirumoorthy (11224754) (author), Robert N. Kirchdoerfer (2418154) (author), Joshua J. Coon (388014) (author), Kavi P. M. Mehta (22075944) (author), Kinjal Majumder (9530459) (author)
Argitaratua:	2025
Gaiak:	Biophysics Biochemistry Microbiology Cell Biology Genetics Molecular Biology Cancer Science Policy Mental Health Infectious Diseases Plant Biology Virology Biological Sciences not elsewhere classified therapeutic transgene delivery rna processing machinery mediated knockdown leads increasing rep 68 dna repair factors derived oncolytic agents brdu analogs followed replication stress proteins induced replication stress 78 gene expression supports virus replication induce replication stress competent wtaav2 leads associated virus 2 cellular replication forks stranded dna viruses div >< p host replication forks cell replication stress rpa rescues wtaav2 raav2 infected cells associated virus induce host host cells ectopic expression rpa molecules rpa activity cellular stores cellular replisomes host genome host chromosome wtaav2 genome replicative wtaav2 pulsed wtaav2 taken together rodent parvovirus replicative wild raav2 genomes proteomic analysis parvoviridae </ molecule imaging findings suggest family induces empty capsids elevated levels dependent manner chemical inhibition
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Antzeko izenburuak

Mechanism of wtAAV2-induced RPA exhaustion that leads to replication stress on the host genome.
nork: Monnette F. Summers (22075935)
Argitaratua: (2025)

wtAAV2-mediated RPA32 exhaustion induces cellular DNA damage.
nork: Monnette F. Summers (22075935)
Argitaratua: (2025)

wtAAV2 genomes associate with cellular RPA and RPA overexpression rescues AAV2-induced replication stress.
nork: Monnette F. Summers (22075935)
Argitaratua: (2025)

Characterization of the wtAAV2-induced alterations to the human replisome using iPOND-MS.
nork: Monnette F. Summers (22075935)
Argitaratua: (2025)

Recombinant AAV2 vector genomes induce replication stress in host cells.
nork: Monnette F. Summers (22075935)
Argitaratua: (2025)

Replicative and non-replicative wtAAV2 genomes induce replication stress in cycling cells.
nork: Monnette F. Summers (22075935)
Argitaratua: (2025)

Imaging of rAAV2/scAAV2 genomes relative to cellular DDR factors.
nork: Monnette F. Summers (22075935)
Argitaratua: (2025)

Impact of wtAAV2/rAAV2/scAAV2 on host genome stability.
nork: Monnette F. Summers (22075935)
Argitaratua: (2025)

pAAV2 transfection induces replication stress and cellular DNA damage response signals.
nork: Monnette F. Summers (22075935)
Argitaratua: (2025)

wtAAV2 infection induces replication stress in A549 and U2OS cells.
nork: Monnette F. Summers (22075935)
Argitaratua: (2025)

Plasmids used in this study.
nork: Parisa D. Mokhtari (22209097)
Argitaratua: (2025)

Bacterial strains used in this study.
nork: Parisa D. Mokhtari (22209097)
Argitaratua: (2025)

Model for the role of Topo IV in completing replication on the chromosome.
nork: Parisa D. Mokhtari (22209097)
Argitaratua: (2025)

Inactivation of Topo IV leads to a specific inability to replicate the terminus region of the chromosome.
nork: Parisa D. Mokhtari (22209097)
Argitaratua: (2025)

Impaired Topo I function leads to amplifications at loci where replication forks converge on the chromosome.
nork: Parisa D. Mokhtari (22209097)
Argitaratua: (2025)

Topo IV mutants accumulate abnormal replication intermediates on substrates with convergent replication forks.
nork: Parisa D. Mokhtari (22209097)
Argitaratua: (2025)

Loss of Topo IV activity impairs the ability to maintain plasmids containing converging replication forks.
nork: Parisa D. Mokhtari (22209097)
Argitaratua: (2025)

Properties of <i>gyrB</i><sup>ts</sup> and <i>parE</i><sup>ts</sup> strains at the permissive, semi-permissive, and restrictive temperatures used in this study.
nork: Parisa D. Mokhtari (22209097)
Argitaratua: (2025)

Mutations in <i>Topo I but not Topo III impair the ability to maintain plasmids containing convergent replication forks.</i>
nork: Parisa D. Mokhtari (22209097)
Argitaratua: (2025)

Data files for reproducing graphs contained in the figures.
nork: Parisa D. Mokhtari (22209097)
Argitaratua: (2025)

High-order intermediates accumulate on 2-replisome plasmids in <i>parE</i><sup>ts</sup> mutants.
nork: Parisa D. Mokhtari (22209097)
Argitaratua: (2025)

Both replisomes are active on the two-replisome plasmid. ter sequences were cloned into the plasmid, pCL01 in a manner that mimics their position on the chromosome, and the plasmids were analyzed by 2D agaose gel analysis as before.
nork: Parisa D. Mokhtari (22209097)
Argitaratua: (2025)

BRCA2 Deficiency and Replication Stress Drive APOBEC3- Mediated Genomic Instability
nork: Shailja Pathania (15105536)
Argitaratua: (2025)

Enriched gene ontology associated with transcriptional response in liver during early and late response to infection.
nork: Gervais Habarugira (8611323)
Argitaratua: (2025)

Distinct transcriptional signatures in liver during early and late response to infection.
nork: Gervais Habarugira (8611323)
Argitaratua: (2025)

Enriched gene ontology associated with transcriptional response in kidney during early and late response to infection.
nork: Gervais Habarugira (8611323)
Argitaratua: (2025)

Distinct transcriptional signatures in the kidney during early and late response to infection.
nork: Gervais Habarugira (8611323)
Argitaratua: (2025)

Principal component analysis (PCA) plots for RNA-seq data of the kidney and liver.
nork: Gervais Habarugira (8611323)
Argitaratua: (2025)

Neutralizing antibody titers in individual experimentally infected and in-pen contact hatchling saltwater crocodiles. The dotted line represents the limit of detection at a titer of 1:20 dilution.
nork: Gervais Habarugira (8611323)
Argitaratua: (2025)

Histopathological lesions in infected saltwater crocodiles.
nork: Gervais Habarugira (8611323)
Argitaratua: (2025)

Lymphoid aggregates in different tissue sections.
nork: Gervais Habarugira (8611323)
Argitaratua: (2025)

Proportion of WNV positive among infected, in-contact control and mock-infected crocodiles.
nork: Gervais Habarugira (8611323)
Argitaratua: (2025)

Viremia and viral load in selected tissues of infected saltwater crocodiles in relation to viremia measured by RT-qPCR at various timepoints.
nork: Gervais Habarugira (8611323)
Argitaratua: (2025)

An illustrative summary of WNV infection kinetics experiment in saltwater crocodile hatchlings.
nork: Gervais Habarugira (8611323)
Argitaratua: (2025)

Pro-inflammatory and anti-inflammatory cytokine gene transcriptional profile in liver, spleen, and kidney at various timepoints post infection.
nork: Gervais Habarugira (8611323)
Argitaratua: (2025)

Cell proliferation and apoptosis cytokine gene transcriptional profile in liver, spleen, and kidney at various timepoints post infection.
nork: Gervais Habarugira (8611323)
Argitaratua: (2025)

Comparison of antiviral cytokine and transcription factor gene transcriptional kinetics in liver, spleen, and kidney at various time points post-infection.
nork: Gervais Habarugira (8611323)
Argitaratua: (2025)

Cell proliferation and apoptosis cytokine gene transcriptional profile in liver, spleen, and kidney tissues at various timepoints.
nork: Gervais Habarugira (8611323)
Argitaratua: (2025)

Pro- and anti-inflammatory cytokine gene transcriptional profile in liver, spleen, and kidney tissues at various timepoints.
nork: Gervais Habarugira (8611323)
Argitaratua: (2025)

Antiviral cytokine and transcription factor gene transcriptional profile in liver, spleen, and kidney at various timepoints.
nork: Gervais Habarugira (8611323)
Argitaratua: (2025)