PrimeFlow RNA assay verified that the sLCL 381 cells are EBV-infected B cells.
<p>1.5 x 10<sup>6</sup> <b>(a)</b> sLCL 381 and <b>(b)</b> sLCL 421 cells were stained by Aqua Blue dye (Invitrogen, USA) and the following fluorochrome-conjugated antibodies: Pacific Blue anti-human CD3, PE-Cy7 anti-human CD8, PE anti-human CD19, FITC anti-...
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2024
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| Summary: | <p>1.5 x 10<sup>6</sup> <b>(a)</b> sLCL 381 and <b>(b)</b> sLCL 421 cells were stained by Aqua Blue dye (Invitrogen, USA) and the following fluorochrome-conjugated antibodies: Pacific Blue anti-human CD3, PE-Cy7 anti-human CD8, PE anti-human CD19, FITC anti-human CD16/56 (BioLegend, USA) and PE-Texas Red anti-CD4 (eBioscience, USA) on ice for 30 minutes for indication of cell viability and immunophenotype. The cells were fixed by fixation buffer and subsequently treated with PrimeFlow RNA permeabilization buffer with RNase inhibitors. The EBER in the cells were stained by fluorochrome-conjugated probe EBER-AF647 (Thermo Fisher Scientific, USA) and the cells were incubated in the dark at 4°C overnight. On the next day, the cells were incubated with PrimeFlow RNA PreAmp Mix, PrimeFlow RNA Amp Mix, and diluted Label Probes for signal amplification. The samples were measured by flow cytometry and analyzed by FlowJo software (Tree Star).</p> <p>(TIF)</p> |
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