RNG2 is functional only as intact, full-length protein.
<p><b>(A)</b> Representation of the RNG2 3 processed products originally identified in insect cells (155kDa, 90kDa, 60kDa) expressed in the Δ<i>rng2</i> background. <b>(B)</b> Western blot analysis of the expression of RNG2 variants expressed in the Δ<i&g...
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2025
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| Sumari: | <p><b>(A)</b> Representation of the RNG2 3 processed products originally identified in insect cells (155kDa, 90kDa, 60kDa) expressed in the Δ<i>rng2</i> background. <b>(B)</b> Western blot analysis of the expression of RNG2 variants expressed in the Δ<i>rng2</i> background. <b>(C)</b> U-ExM images of the localization of the RNG2 variants. Scale bar = 3 μm. <b>(D)</b> Quantification of the plaque sizes created by the different RNG2 strains via a plaque assay. Ten plaques were measured per biological replicate. Three biological replicates were evaluated. The data underlying this figure can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3003506#pbio.3003506.s008" target="_blank">S2 Data</a>. <b>(E)</b> Quantification of the percentage of invasion by the different RNG2 strains in the invasion assay. One hundred parasites were measured per biological replicate. Three biological replicates were evaluated. The data underlying this figure can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3003506#pbio.3003506.s008" target="_blank">S2 Data</a>. <b>(F)</b> Quantification of the percentages of conoid retraction, extrusion, and detachment by the different RNG2 strains in the conoid detachment assay. One hundred parasites were measured per biological replicate. Three biological replicates were evaluated. The data underlying this figure can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3003506#pbio.3003506.s008" target="_blank">S2 Data</a>. <b>(G)</b> Design of the experiment to test if different fragments of RNG2 can cooperate to form a functional tether. <b>(H)</b> Conoid detachment assessed by U-ExM in transiently transfected parasites treated for 48h under auxin to down-regulate the endogenous RNG2-mAID. The data underlying this figure can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3003506#pbio.3003506.s007" target="_blank">S1 Data</a>. <b>(I)</b> Representation of the skip peptide (P2A) variants expressed in the RNG2-mAID-HA strain. <b>(J)</b> Western blot analysis of the expression and skipping pattern verifying the successful skipping of ribosome on the P2A sequence. <b>(K)</b> U-ExM images on auxin treated parasite to deplete the endogenous RNG2, showing the localization of both P2A variants and their ability to maintain the conoid at the apical pole. <b>(L)</b> Quantification of the plaque sizes created by RNG2 P2A variants via a plaque assay. Ten plaques were measured per biological replicate. Three biological replicates were evaluated. The data underlying this figure can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3003506#pbio.3003506.s008" target="_blank">S2 Data</a>.</p> |
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