Linear discriminant analysis effect size (LEfSe) at various taxonomic levels (kingdom (k), phylum (p), class (c), order (o), family (f), genus (g), and species (s)) for broiler chickens fed diets containing fats of plant, animal, and insect origin.

<p>(A) bar graph of LDA scores for three groups (BSFL, plant oil, and animal fats), representing log10-transformed values for differentially abundant taxa; (B) cladogram illustrating phylogenetic relationships between taxa with significant differences in abundance across three groups (BSFL, pl...

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Автор: Muhammad Rumman Aslam (22683482) (author)
Інші автори: Bartosz Kierończyk (500220) (author), Piotr Szymkowiak (22683485) (author), Liliana Ciesielska (22683488) (author), Mateusz Rawski (500223) (author), Robert Mikuła (6595409) (author), Damian Józefiak (500219) (author)
Опубліковано: 2025
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_version_ 1849927628001116160
author Muhammad Rumman Aslam (22683482)
author2 Bartosz Kierończyk (500220)
Piotr Szymkowiak (22683485)
Liliana Ciesielska (22683488)
Mateusz Rawski (500223)
Robert Mikuła (6595409)
Damian Józefiak (500219)
author2_role author
author
author
author
author
author
author_facet Muhammad Rumman Aslam (22683482)
Bartosz Kierończyk (500220)
Piotr Szymkowiak (22683485)
Liliana Ciesielska (22683488)
Mateusz Rawski (500223)
Robert Mikuła (6595409)
Damian Józefiak (500219)
author_role author
dc.creator.none.fl_str_mv Muhammad Rumman Aslam (22683482)
Bartosz Kierończyk (500220)
Piotr Szymkowiak (22683485)
Liliana Ciesielska (22683488)
Mateusz Rawski (500223)
Robert Mikuła (6595409)
Damian Józefiak (500219)
dc.date.none.fl_str_mv 2025-11-25T18:31:04Z
dc.identifier.none.fl_str_mv 10.1371/journal.pone.0336523.g004
dc.relation.none.fl_str_mv https://figshare.com/articles/figure/Linear_discriminant_analysis_effect_size_LEfSe_at_various_taxonomic_levels_kingdom_k_phylum_p_class_c_order_o_family_f_genus_g_and_species_s_for_broiler_chickens_fed_diets_containing_fats_of_plant_animal_and_insect_origin_/30714101
dc.rights.none.fl_str_mv CC BY 4.0
info:eu-repo/semantics/openAccess
dc.subject.none.fl_str_mv Biochemistry
Microbiology
Molecular Biology
Biotechnology
Ecology
Environmental Sciences not elsewhere classified
Biological Sciences not elsewhere classified
Chemical Sciences not elsewhere classified
various dietary fats
selected animal fats
lefse analysis revealed
inhibiting opportunistic pathogens
broilers responds similarly
broiler chicken nutrition
hermetia illucens </
eubacterium coprostanoligenes </
clostridia vadin </
eight dietary groups
10 replicate pens
011 ), enterobacteriaceae
003 ), rhodospirillales
001 ), rikenellaceae
), rapeseed oil
bsfl significantly reduced
bsfl positively affects
including ph determination
div >< p
025 ), peptococcaceae
35 days ),
including bsfl fat
35 days
025 ),
shigella </
alistipes </
pl ),
10 birds
048 ),
032 ),
018 ),
bsfl treatment
bsfl increased
bsfl fat
pkfd groups
upper part
uncultured genus
study design
study aimed
sole source
significant reduction
randomly assigned
ngs ).
gut ph
generation sequencing
findings suggest
detected among
cecal microbiota
cecal microbiome
cecal environment
dc.title.none.fl_str_mv Linear discriminant analysis effect size (LEfSe) at various taxonomic levels (kingdom (k), phylum (p), class (c), order (o), family (f), genus (g), and species (s)) for broiler chickens fed diets containing fats of plant, animal, and insect origin.
dc.type.none.fl_str_mv Image
Figure
info:eu-repo/semantics/publishedVersion
image
description <p>(A) bar graph of LDA scores for three groups (BSFL, plant oil, and animal fats), representing log10-transformed values for differentially abundant taxa; (B) cladogram illustrating phylogenetic relationships between taxa with significant differences in abundance across three groups (BSFL, plant oil, and animal fats). BSFL – basal diet with 100% black soldier fly (<i>Hermetia illucens</i>) larval fat; plant oils (SO – basal diet with 100% soybean oil; RO – basal diet with 100% rapeseed oil; PO – basal diet with 100% palm oil; PKFD – basal diet with 100% palm kernel fat distillate); animal fats (PF – basal diet with 100% poultry fat; PL – basal diet with 100% pig lard; BT – basal diet with 100% beef tallow).</p>
eu_rights_str_mv openAccess
id Manara_ad8ac2e9bb088e50ed8f13e000e46af6
identifier_str_mv 10.1371/journal.pone.0336523.g004
network_acronym_str Manara
network_name_str ManaraRepo
oai_identifier_str oai:figshare.com:article/30714101
publishDate 2025
repository.mail.fl_str_mv
repository.name.fl_str_mv
repository_id_str
rights_invalid_str_mv CC BY 4.0
spelling Linear discriminant analysis effect size (LEfSe) at various taxonomic levels (kingdom (k), phylum (p), class (c), order (o), family (f), genus (g), and species (s)) for broiler chickens fed diets containing fats of plant, animal, and insect origin.Muhammad Rumman Aslam (22683482)Bartosz Kierończyk (500220)Piotr Szymkowiak (22683485)Liliana Ciesielska (22683488)Mateusz Rawski (500223)Robert Mikuła (6595409)Damian Józefiak (500219)BiochemistryMicrobiologyMolecular BiologyBiotechnologyEcologyEnvironmental Sciences not elsewhere classifiedBiological Sciences not elsewhere classifiedChemical Sciences not elsewhere classifiedvarious dietary fatsselected animal fatslefse analysis revealedinhibiting opportunistic pathogensbroilers responds similarlybroiler chicken nutritionhermetia illucens </eubacterium coprostanoligenes </clostridia vadin </eight dietary groups10 replicate pens011 ), enterobacteriaceae003 ), rhodospirillales001 ), rikenellaceae), rapeseed oilbsfl significantly reducedbsfl positively affectsincluding ph determinationdiv >< p025 ), peptococcaceae35 days ),including bsfl fat35 days025 ),shigella </alistipes </pl ),10 birds048 ),032 ),018 ),bsfl treatmentbsfl increasedbsfl fatpkfd groupsupper partuncultured genusstudy designstudy aimedsole sourcesignificant reductionrandomly assignedngs ).gut phgeneration sequencingfindings suggestdetected amongcecal microbiotacecal microbiomececal environment<p>(A) bar graph of LDA scores for three groups (BSFL, plant oil, and animal fats), representing log10-transformed values for differentially abundant taxa; (B) cladogram illustrating phylogenetic relationships between taxa with significant differences in abundance across three groups (BSFL, plant oil, and animal fats). BSFL – basal diet with 100% black soldier fly (<i>Hermetia illucens</i>) larval fat; plant oils (SO – basal diet with 100% soybean oil; RO – basal diet with 100% rapeseed oil; PO – basal diet with 100% palm oil; PKFD – basal diet with 100% palm kernel fat distillate); animal fats (PF – basal diet with 100% poultry fat; PL – basal diet with 100% pig lard; BT – basal diet with 100% beef tallow).</p>2025-11-25T18:31:04ZImageFigureinfo:eu-repo/semantics/publishedVersionimage10.1371/journal.pone.0336523.g004https://figshare.com/articles/figure/Linear_discriminant_analysis_effect_size_LEfSe_at_various_taxonomic_levels_kingdom_k_phylum_p_class_c_order_o_family_f_genus_g_and_species_s_for_broiler_chickens_fed_diets_containing_fats_of_plant_animal_and_insect_origin_/30714101CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/307141012025-11-25T18:31:04Z
spellingShingle Linear discriminant analysis effect size (LEfSe) at various taxonomic levels (kingdom (k), phylum (p), class (c), order (o), family (f), genus (g), and species (s)) for broiler chickens fed diets containing fats of plant, animal, and insect origin.
Muhammad Rumman Aslam (22683482)
Biochemistry
Microbiology
Molecular Biology
Biotechnology
Ecology
Environmental Sciences not elsewhere classified
Biological Sciences not elsewhere classified
Chemical Sciences not elsewhere classified
various dietary fats
selected animal fats
lefse analysis revealed
inhibiting opportunistic pathogens
broilers responds similarly
broiler chicken nutrition
hermetia illucens </
eubacterium coprostanoligenes </
clostridia vadin </
eight dietary groups
10 replicate pens
011 ), enterobacteriaceae
003 ), rhodospirillales
001 ), rikenellaceae
), rapeseed oil
bsfl significantly reduced
bsfl positively affects
including ph determination
div >< p
025 ), peptococcaceae
35 days ),
including bsfl fat
35 days
025 ),
shigella </
alistipes </
pl ),
10 birds
048 ),
032 ),
018 ),
bsfl treatment
bsfl increased
bsfl fat
pkfd groups
upper part
uncultured genus
study design
study aimed
sole source
significant reduction
randomly assigned
ngs ).
gut ph
generation sequencing
findings suggest
detected among
cecal microbiota
cecal microbiome
cecal environment
status_str publishedVersion
title Linear discriminant analysis effect size (LEfSe) at various taxonomic levels (kingdom (k), phylum (p), class (c), order (o), family (f), genus (g), and species (s)) for broiler chickens fed diets containing fats of plant, animal, and insect origin.
title_full Linear discriminant analysis effect size (LEfSe) at various taxonomic levels (kingdom (k), phylum (p), class (c), order (o), family (f), genus (g), and species (s)) for broiler chickens fed diets containing fats of plant, animal, and insect origin.
title_fullStr Linear discriminant analysis effect size (LEfSe) at various taxonomic levels (kingdom (k), phylum (p), class (c), order (o), family (f), genus (g), and species (s)) for broiler chickens fed diets containing fats of plant, animal, and insect origin.
title_full_unstemmed Linear discriminant analysis effect size (LEfSe) at various taxonomic levels (kingdom (k), phylum (p), class (c), order (o), family (f), genus (g), and species (s)) for broiler chickens fed diets containing fats of plant, animal, and insect origin.
title_short Linear discriminant analysis effect size (LEfSe) at various taxonomic levels (kingdom (k), phylum (p), class (c), order (o), family (f), genus (g), and species (s)) for broiler chickens fed diets containing fats of plant, animal, and insect origin.
title_sort Linear discriminant analysis effect size (LEfSe) at various taxonomic levels (kingdom (k), phylum (p), class (c), order (o), family (f), genus (g), and species (s)) for broiler chickens fed diets containing fats of plant, animal, and insect origin.
topic Biochemistry
Microbiology
Molecular Biology
Biotechnology
Ecology
Environmental Sciences not elsewhere classified
Biological Sciences not elsewhere classified
Chemical Sciences not elsewhere classified
various dietary fats
selected animal fats
lefse analysis revealed
inhibiting opportunistic pathogens
broilers responds similarly
broiler chicken nutrition
hermetia illucens </
eubacterium coprostanoligenes </
clostridia vadin </
eight dietary groups
10 replicate pens
011 ), enterobacteriaceae
003 ), rhodospirillales
001 ), rikenellaceae
), rapeseed oil
bsfl significantly reduced
bsfl positively affects
including ph determination
div >< p
025 ), peptococcaceae
35 days ),
including bsfl fat
35 days
025 ),
shigella </
alistipes </
pl ),
10 birds
048 ),
032 ),
018 ),
bsfl treatment
bsfl increased
bsfl fat
pkfd groups
upper part
uncultured genus
study design
study aimed
sole source
significant reduction
randomly assigned
ngs ).
gut ph
generation sequencing
findings suggest
detected among
cecal microbiota
cecal microbiome
cecal environment