Protein expression, purification, and GC-MS analyses for LadA and Fre reaction.

Protein expression, purification, and GC-MS analyses for LadA and Fre reaction.

<p>(A) SDS-PAGE. Lane 1: Molecular weight (MW) marker. Lane 2: purified <i><i>wild-type</i></i> N-strepTag LadA (WP_011888513.1, estimated MW = 51.8 kDa). Lane 3: purified <i><i>wild-type</i></i> N-strepTag Fre (WP_000209826.1, estimated MW = 27....

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Detalhes bibliográficos
Autor principal:	Raul Mireles (21175708) (author)
Outros Autores:	Arne Matthews (22279026) (author), Robin Teufel (1355409) (author), Lianet Noda-García (22279029) (author)
Publicado em:	2025
Assuntos:	Biophysics Biochemistry Microbiology Cell Biology Genetics Molecular Biology Biotechnology Cancer Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified two distinct metal functionalization step could four novel homologs environmentally friendly processes despite extensive efforts >- alkane hydroxylation >- alkane functionalization significant challenge due >- alkane monooxygenases enzyme families known div >< p scalable industrial applications >- alkanes flavoprotein monooxygenases findings challenge environmental applications vitro </ soluble proteins similar conclusions protein superfamily optimized variant one iron n </ inherent stability free flavin first oxo dependent enzymes current understanding covalent bonds binding protein
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