Amino acid sequence alignment (A) and amino acid sequence identity (B) of CHUA_008368 (CYP4GL4) from <i>Chamberlinius hualienensis</i>, Hho_020879 from <i>Helicorthomorpha holstii.</i>

Amino acid sequence alignment (A) and amino acid sequence identity (B) of CHUA_008368 (CYP4GL4) from <i>Chamberlinius hualienensis</i>, Hho_020879 from <i>Helicorthomorpha holstii.</i>

<p>Amino acid sequence alignment (A) and amino acid sequence identity (B) of CHUA_008368 (CYP4GL4) from <i>Chamberlinius hualienensis</i>, Hho_020879 from <i>Helicorthomorpha holstii.</i></p>

Wedi'i Gadw mewn:

Manylion Llyfryddiaeth
Prif Awdur:	Takuya Yamaguchi (4543105) (author)
Awduron Eraill:	Yasuhisa Asano (727606) (author)
Cyhoeddwyd:	2025
Pynciau:	Biochemistry Evolutionary Biology Ecology Plant Biology Virology Biological Sciences not elsewhere classified Chemical Sciences not elsewhere classified subsequently converting aldoxime natural defensive chemicals indicating sequential duplication catalyse aldoxime dehydration div >< p cyanogenic millipedes evolved chamberlinius hualienensis </ hualienensis </ r </ typically lineage related enzymes plant lineages plant kingdoms paralogous genes millipedes demonstrates mechanisms underlying hydroxynitrile lyase hydrogen cyanide giving rise enzymes independently dependent monooxygenase deeper understanding cytochrome p450 complete set coding genes biological characterisation >)- mandelonitrile
Tagiau:	Ychwanegu Tag Dim Tagiau, Byddwch y cyntaf i dagio'r cofnod hwn!

Eitemau Tebyg

Amino acid sequence alignment (A) and amino acid sequence identity (B) of CHUA_003298 (ChuaMOxS) from <i>Chamberlinius hualienensis</i>, Hho_019011 from <i>Helicorthomorpha holstii</i>, and Nno_033811 from <i>Niponia nodulosa.</i>
gan: Takuya Yamaguchi (4543105)
Cyhoeddwyd: (2025)

Amino acid sequence alignment (A) and and amino acid sequence identity (B) of CHUA_014123 (CYP30008A2) from <i>Chamberlinius hualienensis</i> and its orthorogous proteins, Hho_011653 from <i>Helicorthomorpha holstii</i> and Nno_033394 from <i>Niponia nodulosa.</i>
gan: Takuya Yamaguchi (4543105)
Cyhoeddwyd: (2025)

Amino acid sequence alignment (A) and sequence identity matrix (B) of <i>Chamberliniensis hualienensis</i> flavin-dependent monooxygenases.
gan: Takuya Yamaguchi (4543105)
Cyhoeddwyd: (2025)

Amino acid sequence alignment of ChuaHNL and its paralogous proteins.
gan: Takuya Yamaguchi (4543105)
Cyhoeddwyd: (2025)

Amino acid sequence identity matrix of ChuaHNL and its paralogous proteins.
gan: Takuya Yamaguchi (4543105)
Cyhoeddwyd: (2025)

Detection of aromatic amino acids-derived aldoximes after the culture of <i>Escherichia coli</i> harboring millipede aldoxime synthase (MOxS, CHUA_003298).
gan: Takuya Yamaguchi (4543105)
Cyhoeddwyd: (2025)

Characterization of CYP4GL4 (CHUA_008368) and CYP30008A2 (CHUA_014123) catalyzing dehydration of (<i>E/Z</i>)-phenylacetaldoxime (PAOx) into phenylacetonitrile.
gan: Takuya Yamaguchi (4543105)
Cyhoeddwyd: (2025)

Formation of phenylacetonitrile (PAN) from (<i>E/Z</i>)-phenylacetaldoxime by yeast harboring CHUA_008368 (CYP4GL4) and CHUA_014123 (CYP30008A2).
gan: Takuya Yamaguchi (4543105)
Cyhoeddwyd: (2025)

Substrate specificity of CYP4GL4 (CHUA_008368) and CYP30008A2 (CHUA_014123) toward aromatic aldoximes.
gan: Takuya Yamaguchi (4543105)
Cyhoeddwyd: (2025)

Shotgun sequencing summary statistics (Illumina).
gan: Takuya Yamaguchi (4543105)
Cyhoeddwyd: (2025)

Shotgun sequencing summary statistics (PacBio).
gan: Takuya Yamaguchi (4543105)
Cyhoeddwyd: (2025)

Body weight and accumulation of (<i>R</i>)-mandelonitrile in <i>Chamberlinius hualienensis.</i>
gan: Takuya Yamaguchi (4543105)
Cyhoeddwyd: (2025)

Identification of (<i>R</i>)-mandelonitrile biosynthetic enzymes from <i>C. hualienensis.</i>
gan: Takuya Yamaguchi (4543105)
Cyhoeddwyd: (2025)

<i>C. hualienensis</i> genome annotation.
gan: Takuya Yamaguchi (4543105)
Cyhoeddwyd: (2025)

Assembly and annotation statistics of <i>Chamberlinius hualienensis</i> genome.
gan: Takuya Yamaguchi (4543105)
Cyhoeddwyd: (2025)

Comparison of phenylacetonitrile (PAN) metabolizing activity of <i>Saccharomyces cerevisiae</i> ChCR11 and INVSc1 expressing <i>CYP3201B1.</i>
gan: Takuya Yamaguchi (4543105)
Cyhoeddwyd: (2025)

Gene cluster and characterisation of <i>ChuaHNL</i> and its paralogues.
gan: Takuya Yamaguchi (4543105)
Cyhoeddwyd: (2025)

TMM normalized read counts of (<i>R</i>)-mandelonitrile biosynthetic genes.
gan: Takuya Yamaguchi (4543105)
Cyhoeddwyd: (2025)

Characteristics of flavin-dependent monooxygenases (FMOs) from <i>Chamberlinius hualienensis.</i>
gan: Takuya Yamaguchi (4543105)
Cyhoeddwyd: (2025)

Heterologous production of ChuaHNL and its paralogous proteins in <i>Pichia pastoris.</i>
gan: Takuya Yamaguchi (4543105)
Cyhoeddwyd: (2025)

Substrate saturation curves for CHUA_005138 (ChuaHNL), CHUA_005137, and CHUA_005136 with (<i>R,S</i>)-mandelonitrile (mandelonitrile cleavage reaction) (A) or benzaldehyde ((<i>R</i>)-mandelonitrile synthetic reaction) (B).
gan: Takuya Yamaguchi (4543105)
Cyhoeddwyd: (2025)

Schematic overview of cyanogenesis in <i>Chamberlinius hualienensis</i> and cyanohydrin biosynthesis in millipedes, insects, and plants.
gan: Takuya Yamaguchi (4543105)
Cyhoeddwyd: (2025)

Identification of phenylacetaldoxime (PAOx)-producing flavin dependent monooxygenase from <i>Chamberlinius hualienensis.</i>
gan: Takuya Yamaguchi (4543105)
Cyhoeddwyd: (2025)

GenomeScope plot of the 21-mer content within the <i>C. hualienensis</i> genome.
gan: Takuya Yamaguchi (4543105)
Cyhoeddwyd: (2025)

Overview of the processing pipeline used for the assembly of the <i>Chamberlinius hualienensis genome</i> (see materials and methods for detail).
gan: Takuya Yamaguchi (4543105)
Cyhoeddwyd: (2025)

(<i>R</i>)-mandelonitrile synthetic reaction catalyzed by CHUA_005138 (ChuaHNL), CHUA_005137, and CHUA_005136.
gan: Takuya Yamaguchi (4543105)
Cyhoeddwyd: (2025)

Purification of N-terminal His-tagged CHUA_005136 from 1.5 L of <i>Pichia pastoris</i> culture supernatant.
gan: Takuya Yamaguchi (4543105)
Cyhoeddwyd: (2025)

Purification of N-terminal His-tagged CHUA_005137 from 1.5 L of <i>Pichia pastoris</i> culture supernatant.
gan: Takuya Yamaguchi (4543105)
Cyhoeddwyd: (2025)

Purification of N-terminal His-tagged ChuaHNL (CHUA_005138) from 1.5 L of <i>Pichia pastoris</i> culture supernatant.
gan: Takuya Yamaguchi (4543105)
Cyhoeddwyd: (2025)

Distribution of cyanogenesis-related enzymes in millipedes.
gan: Takuya Yamaguchi (4543105)
Cyhoeddwyd: (2025)

Numerical data underlying Figs 3, 4E, S4, S8, S10, S12, S13,
gan: Takuya Yamaguchi (4543105)
Cyhoeddwyd: (2025)

TMM normalized read counts of flavin-dependent monooxygenases (FMOs).
gan: Takuya Yamaguchi (4543105)
Cyhoeddwyd: (2025)

RNA-seq for gene expression analysis.
gan: Takuya Yamaguchi (4543105)
Cyhoeddwyd: (2025)

RNA-seq for gene annotation.
gan: Takuya Yamaguchi (4543105)
Cyhoeddwyd: (2025)

TE contents of millipede and centipede.
gan: Takuya Yamaguchi (4543105)
Cyhoeddwyd: (2025)

Flavin-dependent monooxygenases in myriapods and other species.
gan: Takuya Yamaguchi (4543105)
Cyhoeddwyd: (2025)

Phylogenetic tree of cytochrome P450s.
gan: Takuya Yamaguchi (4543105)
Cyhoeddwyd: (2025)

Three dimensional structures of hydroxynitrile lyase (HNL) from millipede, plant, and microorganism.
gan: Takuya Yamaguchi (4543105)
Cyhoeddwyd: (2025)

Phylogenetic tree of millipede HNLs, their paralogous proteins, and lipocalins from animals (arthropoda and chordata), plants, and bacteria.
gan: Takuya Yamaguchi (4543105)
Cyhoeddwyd: (2025)

Correlations of genome sizes and TE contents among millipedes and centipedes.
gan: Takuya Yamaguchi (4543105)
Cyhoeddwyd: (2025)

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