Nitrocefin and p-nitrophenyl butyrate hydrolysis assays for Pseudomonas <i>aeruginosa</i> TesA.
<p><b>(A)</b> Purified <i>P. aeruginosa</i> TesA with N-terminal tag (PaTesA). <b>(B)</b> Representative example of nitrocefin hydrolysis by PaTesA. Red nested curves represent a series of spectral measurements taken over time. Blue curves indicate the contr...
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2025
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| Gaia: | <p><b>(A)</b> Purified <i>P. aeruginosa</i> TesA with N-terminal tag (PaTesA). <b>(B)</b> Representative example of nitrocefin hydrolysis by PaTesA. Red nested curves represent a series of spectral measurements taken over time. Blue curves indicate the control, where the enzyme was substituted for protein-free buffer. <b>(C)</b> Plots of the change in absorption at 488 nm for enzyme-catalyzed reaction (<i>red</i> dots) and the control (<i>blue</i> dots). A fit from a single exponential function is shown in black. <b>(D)</b> Photograph of two wells after ~18h showing the color change associated with nitrocefin hydrolysis. Underlying data is provided in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3003427#pbio.3003427.s028" target="_blank">S11 Data</a>–<a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3003427#pbio.3003427.s030" target="_blank">S13 Data</a>.</p> <p>(TIFF)</p> |
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