Nitrocefin and p-nitrophenyl butyrate hydrolysis assays for Pseudomonas aeruginosa TesA.

(A) Purified P. aeruginosa TesA with N-terminal tag (PaTesA). (B) Representative example of nitrocefin hydrolysis by PaTesA. Red nested curves represent a series of spectral measurements taken over time. Blue curves indicate the contr...

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التفاصيل البيبلوغرافية
المؤلف الرئيسي:	Martin B. L. McAndrew (22683641) (author)
مؤلفون آخرون:	Jonathan Cook (3435386) (author), Amy Gill (14910614) (author), Kavya Sahoo (22683644) (author), Clare Thomas (7943747) (author), Phillip J. Stansfeld (1267617) (author), Allister Crow (263807) (author)
منشور في:	2025
الموضوعات:	Biophysics Biochemistry Microbiology Cell Biology Molecular Biology Biotechnology Developmental Biology Cancer Hematology Biological Sciences not elsewhere classified Chemical Sciences not elsewhere classified Information Systems not elsewhere classified tolc efflux pump multifunctional hydrolytic enzyme extracts hydrophobic compounds extracting hydrophobic molecules capture implied long bacterial cell envelope active site residues lipid hydrolase complex escherichia coli </ data suggests ybbap abc superfamily ’ div >< p coli </ abc superfamily thioester substrates tesa complex structural characterization remarkable diversity new function mechanotransmission mechanism inner membrane extensively characterized experimentally confirm complete ybbap >, three
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الملخص:	<p><b>(A)</b> Purified <i>P. aeruginosa</i> TesA with N-terminal tag (PaTesA). <b>(B)</b> Representative example of nitrocefin hydrolysis by PaTesA. Red nested curves represent a series of spectral measurements taken over time. Blue curves indicate the control, where the enzyme was substituted for protein-free buffer. <b>(C)</b> Plots of the change in absorption at 488 nm for enzyme-catalyzed reaction (<i>red</i> dots) and the control (<i>blue</i> dots). A fit from a single exponential function is shown in black. <b>(D)</b> Photograph of two wells after ~18h showing the color change associated with nitrocefin hydrolysis. Underlying data is provided in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3003427#pbio.3003427.s028" target="_blank">S11 Data</a>–<a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3003427#pbio.3003427.s030" target="_blank">S13 Data</a>.</p> <p>(TIFF)</p>

Nitrocefin and p-nitrophenyl butyrate hydrolysis assays for Pseudomonas <i>aeruginosa</i> TesA.

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