Electrochemical Impedance Spectroscopic Analysis of Diffusion-Layer Thickness Distribution Associated with Flagellar Motion of <i>Volvox carteri</i> (Supporting Information)

<p dir="ltr">Although electrochemical methods have been used to relate microbial motility with ionic or redox signals, the evaluation of diffusion-layer variations induced by such motility using electrochemical impedance spectroscopy (EIS) remains limited. Thus, in this study, EIS is...

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محفوظ في:
التفاصيل البيبلوغرافية
المؤلف الرئيسي: Isao SHITANDA (22326749) (author)
مؤلفون آخرون: Koji ISHIZAKI (22326753) (author), Hikari WATANABE (22326756) (author), Masayuki ITAGAKI (22326759) (author)
منشور في: 2025
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author Isao SHITANDA (22326749)
author2 Koji ISHIZAKI (22326753)
Hikari WATANABE (22326756)
Masayuki ITAGAKI (22326759)
author2_role author
author
author
author_facet Isao SHITANDA (22326749)
Koji ISHIZAKI (22326753)
Hikari WATANABE (22326756)
Masayuki ITAGAKI (22326759)
author_role author
dc.creator.none.fl_str_mv Isao SHITANDA (22326749)
Koji ISHIZAKI (22326753)
Hikari WATANABE (22326756)
Masayuki ITAGAKI (22326759)
dc.date.none.fl_str_mv 2025-10-07T01:05:02Z
dc.identifier.none.fl_str_mv 10.50892/data.electrochemistry.30228937.v1
dc.relation.none.fl_str_mv https://figshare.com/articles/dataset/Electrochemical_Impedance_Spectroscopic_Analysis_of_Diffusion-Layer_Thickness_Distribution_Associated_with_Flagellar_Motion_of_i_Volvox_carteri_i_Supporting_Information_/30228937
dc.rights.none.fl_str_mv CC BY 4.0
info:eu-repo/semantics/openAccess
dc.subject.none.fl_str_mv Chemistry
Bioconvection
Diffusion Layer
Electrochemical Impedance Spectroscopy
Distributed Equivalent Circuit
dc.title.none.fl_str_mv Electrochemical Impedance Spectroscopic Analysis of Diffusion-Layer Thickness Distribution Associated with Flagellar Motion of <i>Volvox carteri</i> (Supporting Information)
dc.type.none.fl_str_mv Dataset
info:eu-repo/semantics/publishedVersion
dataset
description <p dir="ltr">Although electrochemical methods have been used to relate microbial motility with ionic or redox signals, the evaluation of diffusion-layer variations induced by such motility using electrochemical impedance spectroscopy (EIS) remains limited. Thus, in this study, EIS is used to investigate how the flagellar activity of <i>Volvox carteri</i> modulates the diffusion layer above an electrode. Finite-element simulations and distributed equivalent-circuit modeling are performed for both uniform and non-uniform diffusion-layer thicknesses. Simulations predict that phototactic flagellar convection results in impedance spectra with a slope lower than 45° in the mid-frequency region and a pronounced finite-diffusion bend at low frequencies. These features are validated through experiments involving <i>Volvox</i>-immobilized electrodes under dark and illuminated conditions. Light irradiation reduces the effective diffusion-layer thickness from 2.4 × 10<sup>−2</sup> cm to 6.6 × 10<sup>−3</sup> cm and introduces a distributed thickness ranging from 4.5 × 10<sup>−4</sup> to 9.0 × 10<sup>−3</sup> cm, as extracted by transmission-line fitting. These results provide quantitative insights into bio-induced mass-transport modulation and demonstrate the applicability of distributed diffusion models in bioelectrochemical systems.</p>
eu_rights_str_mv openAccess
id Manara_b0914bb1699fe3515dfd64bd752c46b0
identifier_str_mv 10.50892/data.electrochemistry.30228937.v1
network_acronym_str Manara
network_name_str ManaraRepo
oai_identifier_str oai:figshare.com:article/30228937
publishDate 2025
repository.mail.fl_str_mv
repository.name.fl_str_mv
repository_id_str
rights_invalid_str_mv CC BY 4.0
spelling Electrochemical Impedance Spectroscopic Analysis of Diffusion-Layer Thickness Distribution Associated with Flagellar Motion of <i>Volvox carteri</i> (Supporting Information)Isao SHITANDA (22326749)Koji ISHIZAKI (22326753)Hikari WATANABE (22326756)Masayuki ITAGAKI (22326759)ChemistryBioconvectionDiffusion LayerElectrochemical Impedance SpectroscopyDistributed Equivalent Circuit<p dir="ltr">Although electrochemical methods have been used to relate microbial motility with ionic or redox signals, the evaluation of diffusion-layer variations induced by such motility using electrochemical impedance spectroscopy (EIS) remains limited. Thus, in this study, EIS is used to investigate how the flagellar activity of <i>Volvox carteri</i> modulates the diffusion layer above an electrode. Finite-element simulations and distributed equivalent-circuit modeling are performed for both uniform and non-uniform diffusion-layer thicknesses. Simulations predict that phototactic flagellar convection results in impedance spectra with a slope lower than 45° in the mid-frequency region and a pronounced finite-diffusion bend at low frequencies. These features are validated through experiments involving <i>Volvox</i>-immobilized electrodes under dark and illuminated conditions. Light irradiation reduces the effective diffusion-layer thickness from 2.4 × 10<sup>−2</sup> cm to 6.6 × 10<sup>−3</sup> cm and introduces a distributed thickness ranging from 4.5 × 10<sup>−4</sup> to 9.0 × 10<sup>−3</sup> cm, as extracted by transmission-line fitting. These results provide quantitative insights into bio-induced mass-transport modulation and demonstrate the applicability of distributed diffusion models in bioelectrochemical systems.</p>2025-10-07T01:05:02ZDatasetinfo:eu-repo/semantics/publishedVersiondataset10.50892/data.electrochemistry.30228937.v1https://figshare.com/articles/dataset/Electrochemical_Impedance_Spectroscopic_Analysis_of_Diffusion-Layer_Thickness_Distribution_Associated_with_Flagellar_Motion_of_i_Volvox_carteri_i_Supporting_Information_/30228937CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/302289372025-10-07T01:05:02Z
spellingShingle Electrochemical Impedance Spectroscopic Analysis of Diffusion-Layer Thickness Distribution Associated with Flagellar Motion of <i>Volvox carteri</i> (Supporting Information)
Isao SHITANDA (22326749)
Chemistry
Bioconvection
Diffusion Layer
Electrochemical Impedance Spectroscopy
Distributed Equivalent Circuit
status_str publishedVersion
title Electrochemical Impedance Spectroscopic Analysis of Diffusion-Layer Thickness Distribution Associated with Flagellar Motion of <i>Volvox carteri</i> (Supporting Information)
title_full Electrochemical Impedance Spectroscopic Analysis of Diffusion-Layer Thickness Distribution Associated with Flagellar Motion of <i>Volvox carteri</i> (Supporting Information)
title_fullStr Electrochemical Impedance Spectroscopic Analysis of Diffusion-Layer Thickness Distribution Associated with Flagellar Motion of <i>Volvox carteri</i> (Supporting Information)
title_full_unstemmed Electrochemical Impedance Spectroscopic Analysis of Diffusion-Layer Thickness Distribution Associated with Flagellar Motion of <i>Volvox carteri</i> (Supporting Information)
title_short Electrochemical Impedance Spectroscopic Analysis of Diffusion-Layer Thickness Distribution Associated with Flagellar Motion of <i>Volvox carteri</i> (Supporting Information)
title_sort Electrochemical Impedance Spectroscopic Analysis of Diffusion-Layer Thickness Distribution Associated with Flagellar Motion of <i>Volvox carteri</i> (Supporting Information)
topic Chemistry
Bioconvection
Diffusion Layer
Electrochemical Impedance Spectroscopy
Distributed Equivalent Circuit