Purification of FP in complexes with cloprostenol.
<p><b>(A)</b> Cartoon of the steps leading to FP purification by IMAC and characterization using HPLC-SEC and microscale fluorescence thermal assay. <b>(B)</b> Representative HPLC-SEC chromatogram of purified FP-WT (Wild Type) in the presence and absence of 100 μM of cl...
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2025
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| 要約: | <p><b>(A)</b> Cartoon of the steps leading to FP purification by IMAC and characterization using HPLC-SEC and microscale fluorescence thermal assay. <b>(B)</b> Representative HPLC-SEC chromatogram of purified FP-WT (Wild Type) in the presence and absence of 100 μM of cloprostenol. The mean ± SEM of FP purification yield determined from 3 independent experiments is 0.097 ± 0.008 and 0.25 ± 0.02 mg/ L for the apo and the cloprostenol-bound forms, respectively. <b>(C)</b> Representative thermostability curve of FP-cloprostenol complex at cloprostenol/ FP molar ratio of 300 (100 μM of cloprostenol) and 0.1 (0.02 μM of cloprostenol). The mean ± SEM of the Tm value determined from 3 independent experiments is 50.8 ± 0.4<sup>o</sup>C and 42.6 ± 0.3<sup>o</sup>C at molar ratios of 300 and 0.1, respectively.</p> |
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