Purification of FP in complexes with cloprostenol.

Purification of FP in complexes with cloprostenol.

<p><b>(A)</b> Cartoon of the steps leading to FP purification by IMAC and characterization using HPLC-SEC and microscale fluorescence thermal assay. <b>(B)</b> Representative HPLC-SEC chromatogram of purified FP-WT (Wild Type) in the presence and absence of 100 μM of cl...

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Dettagli Bibliografici
Autore principale: Marine Salze (8467257) (author)
Altri autori: Sébastien Chrétien (21743678) (author), Tegvir S. Boora (21743681) (author), Madalina Macovei (21743684) (author), Eric Barbeau (21743687) (author), Véronique Blais (7388231) (author), Stéphane A. Laporte (7654517) (author), Martin Audet (192220) (author)
Pubblicazione: 2025
Soggetti:
Biophysics
Biochemistry
Cell Biology
Genetics
Molecular Biology
Biotechnology
Immunology
Developmental Biology
Infectious Diseases
Computational Biology
Chemical Sciences not elsewhere classified
vascular tone regulation
third intracellular loop
mediates signaling events
div >< p
protein engineering effort
icl3 ), n
single particle cryo
pursuing alternative strategies
orthosteric binding site
bioactive lipid derived
fp protein sequence
g protein
fp ),
vitro </
threefold improvement
structural biology
ray crystallography
purified fp
prostaglandin f
point mutations
pathophysiological processes
new x
many physiological
ligand complexes
fully understand
electron microscopy
different efficacies
dependent plasticity
atomic level
arachidonic acid
5 mg
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