Purification of FP in complexes with cloprostenol.

(A) Cartoon of the steps leading to FP purification by IMAC and characterization using HPLC-SEC and microscale fluorescence thermal assay. (B) Representative HPLC-SEC chromatogram of purified FP-WT (Wild Type) in the presence and absence of 100 μM of cl...

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Detalhes bibliográficos
Autor principal:	Marine Salze (8467257) (author)
Outros Autores:	Sébastien Chrétien (21743678) (author), Tegvir S. Boora (21743681) (author), Madalina Macovei (21743684) (author), Eric Barbeau (21743687) (author), Véronique Blais (7388231) (author), Stéphane A. Laporte (7654517) (author), Martin Audet (192220) (author)
Publicado em:	2025
Assuntos:	Biophysics Biochemistry Cell Biology Genetics Molecular Biology Biotechnology Immunology Developmental Biology Infectious Diseases Computational Biology Chemical Sciences not elsewhere classified vascular tone regulation third intracellular loop mediates signaling events div >< p protein engineering effort icl3 ), n single particle cryo pursuing alternative strategies orthosteric binding site bioactive lipid derived fp protein sequence g protein fp ), vitro </ threefold improvement structural biology ray crystallography purified fp prostaglandin f point mutations pathophysiological processes new x many physiological ligand complexes fully understand electron microscopy different efficacies dependent plasticity atomic level arachidonic acid 5 mg
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Purification of FP in complexes with cloprostenol.

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