Electrophysiological development and functional plasticity in dissociated human cerebral organoids across multiple cell lines - Well average data and metadata (1 of 4)
<p dir="ltr">Microelectrode arrays (MEAs) are increasingly used to profile the development of synchronised activity in neural organoids, yet no organoid study has investigated the consistency of electrophysiological development across cell lines. Here, we used dissociated neural orga...
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2025
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| _version_ | 1851480809235546112 |
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| author | Adam Pavlinek (20917388) |
| author2 | Sara Guerrisi (22276579) Kara O'Driscoll (22445599) Deepak P. Srivastava (10025745) Lucia Dutan Polit (5556476) Anthony C Vernon (14184062) Roland Nagy (6489917) Madeline A Lancaster (11284485) |
| author2_role | author author author author author author author |
| author_facet | Adam Pavlinek (20917388) Sara Guerrisi (22276579) Kara O'Driscoll (22445599) Deepak P. Srivastava (10025745) Lucia Dutan Polit (5556476) Anthony C Vernon (14184062) Roland Nagy (6489917) Madeline A Lancaster (11284485) |
| author_role | author |
| dc.creator.none.fl_str_mv | Adam Pavlinek (20917388) Sara Guerrisi (22276579) Kara O'Driscoll (22445599) Deepak P. Srivastava (10025745) Lucia Dutan Polit (5556476) Anthony C Vernon (14184062) Roland Nagy (6489917) Madeline A Lancaster (11284485) |
| dc.date.none.fl_str_mv | 2025-11-25T16:36:17Z |
| dc.identifier.none.fl_str_mv | 10.18742/30394168.v1 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/dataset/Electrophysiological_development_and_functional_plasticity_in_dissociated_human_cerebral_organoids_across_multiple_cell_lines_-_Well_average_data_and_metadata_1_of_4_/30394168 |
| dc.rights.none.fl_str_mv | CC0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Cell development, proliferation and death Cell neurochemistry Biochemistry and cell biology not elsewhere classified human induced pluripotent stem cells micro-electrode array (MEA) cerebral organoid model neuronal culture functional connectivity network dynamics electrophysiology synaptic plasticity |
| dc.title.none.fl_str_mv | Electrophysiological development and functional plasticity in dissociated human cerebral organoids across multiple cell lines - Well average data and metadata (1 of 4) |
| dc.type.none.fl_str_mv | Dataset info:eu-repo/semantics/publishedVersion dataset |
| description | <p dir="ltr">Microelectrode arrays (MEAs) are increasingly used to profile the development of synchronised activity in neural organoids, yet no organoid study has investigated the consistency of electrophysiological development across cell lines. Here, we used dissociated neural organoids derived from six cell lines on MEAs to characterise functional synapse development using multiple parameters across time. The dissociated organoids had increasing functional connectivity and network activity over time across all cell lines and plasticity in response to synaptic-like stimulation. Like the organoids they were derived from, dissociated organoid cultures contained a diverse mixture of cell types. Variability in activity parameters was associated with differences in cell type composition and regional identity, which in turn were affected by donor cell line and batch effects. These results demonstrate that dissociated cerebral organoids can generate functional neurons, akin to primary neuronal cultures from brain tissue, providing a scalable model for studies of neurodevelopment and synaptic function.</p><p dir="ltr">The raw and well average data deposited here consists of 5-minute recordings of dissociated organoids on MEAs over time, as well as a chemical LTP plasticity treatment.</p> |
| eu_rights_str_mv | openAccess |
| id | Manara_b1b88a862ec88c80a510990d4536a78c |
| identifier_str_mv | 10.18742/30394168.v1 |
| network_acronym_str | Manara |
| network_name_str | ManaraRepo |
| oai_identifier_str | oai:figshare.com:article/30394168 |
| publishDate | 2025 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC0 |
| spelling | Electrophysiological development and functional plasticity in dissociated human cerebral organoids across multiple cell lines - Well average data and metadata (1 of 4)Adam Pavlinek (20917388)Sara Guerrisi (22276579)Kara O'Driscoll (22445599)Deepak P. Srivastava (10025745)Lucia Dutan Polit (5556476)Anthony C Vernon (14184062)Roland Nagy (6489917)Madeline A Lancaster (11284485)Cell development, proliferation and deathCell neurochemistryBiochemistry and cell biology not elsewhere classifiedhuman induced pluripotent stem cellsmicro-electrode array (MEA)cerebral organoid modelneuronal culturefunctional connectivitynetwork dynamicselectrophysiologysynaptic plasticity<p dir="ltr">Microelectrode arrays (MEAs) are increasingly used to profile the development of synchronised activity in neural organoids, yet no organoid study has investigated the consistency of electrophysiological development across cell lines. Here, we used dissociated neural organoids derived from six cell lines on MEAs to characterise functional synapse development using multiple parameters across time. The dissociated organoids had increasing functional connectivity and network activity over time across all cell lines and plasticity in response to synaptic-like stimulation. Like the organoids they were derived from, dissociated organoid cultures contained a diverse mixture of cell types. Variability in activity parameters was associated with differences in cell type composition and regional identity, which in turn were affected by donor cell line and batch effects. These results demonstrate that dissociated cerebral organoids can generate functional neurons, akin to primary neuronal cultures from brain tissue, providing a scalable model for studies of neurodevelopment and synaptic function.</p><p dir="ltr">The raw and well average data deposited here consists of 5-minute recordings of dissociated organoids on MEAs over time, as well as a chemical LTP plasticity treatment.</p>2025-11-25T16:36:17ZDatasetinfo:eu-repo/semantics/publishedVersiondataset10.18742/30394168.v1https://figshare.com/articles/dataset/Electrophysiological_development_and_functional_plasticity_in_dissociated_human_cerebral_organoids_across_multiple_cell_lines_-_Well_average_data_and_metadata_1_of_4_/30394168CC0info:eu-repo/semantics/openAccessoai:figshare.com:article/303941682025-11-25T16:36:17Z |
| spellingShingle | Electrophysiological development and functional plasticity in dissociated human cerebral organoids across multiple cell lines - Well average data and metadata (1 of 4) Adam Pavlinek (20917388) Cell development, proliferation and death Cell neurochemistry Biochemistry and cell biology not elsewhere classified human induced pluripotent stem cells micro-electrode array (MEA) cerebral organoid model neuronal culture functional connectivity network dynamics electrophysiology synaptic plasticity |
| status_str | publishedVersion |
| title | Electrophysiological development and functional plasticity in dissociated human cerebral organoids across multiple cell lines - Well average data and metadata (1 of 4) |
| title_full | Electrophysiological development and functional plasticity in dissociated human cerebral organoids across multiple cell lines - Well average data and metadata (1 of 4) |
| title_fullStr | Electrophysiological development and functional plasticity in dissociated human cerebral organoids across multiple cell lines - Well average data and metadata (1 of 4) |
| title_full_unstemmed | Electrophysiological development and functional plasticity in dissociated human cerebral organoids across multiple cell lines - Well average data and metadata (1 of 4) |
| title_short | Electrophysiological development and functional plasticity in dissociated human cerebral organoids across multiple cell lines - Well average data and metadata (1 of 4) |
| title_sort | Electrophysiological development and functional plasticity in dissociated human cerebral organoids across multiple cell lines - Well average data and metadata (1 of 4) |
| topic | Cell development, proliferation and death Cell neurochemistry Biochemistry and cell biology not elsewhere classified human induced pluripotent stem cells micro-electrode array (MEA) cerebral organoid model neuronal culture functional connectivity network dynamics electrophysiology synaptic plasticity |