Methylation specific PCR is reproducible within a wide dynamic range.

A Both detection of a single copy gene and rDNA are linear between 0.3 ng and 20 ng per reaction. The single copy gene ACTB is insensitive to methylation status and detects the same copies/µL with and without M.SssI treatment. Methylated rDNA has lower...

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Bibliographic Details
Main Author:	Stephen Flint Smith (22614954) (author)
Other Authors:	Bernardo Lemos (223826) (author)
Published:	2025
Subjects:	Biophysics Biochemistry Cell Biology Genetics Molecular Biology Cancer Plant Biology least four cpgs found significant correlations bisulfite pcr targeting 20 ng dna 15 ng dna rdna transcriptional activity single copy gene observed wide variation unmethylated rdna cn total rdna copies total rdna cn detecting rdna cn humans &# 160 rdna methylation variability rdna methylation status methylated rdna copies methylated rdna cn rdna copies rdna cn rdna methylation methylated copies 3 ng natural variation copy number within species varies widely simultaneous quantification simultaneous detection scalable approach practical range partially regulated offering insight new tool modified methyl method provides linear range healthy donors greater number
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Methylation specific PCR is reproducible within a wide dynamic range.

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