Stop codon readthrough for tRNA modification gene deletion mutants.

<p>Black: wild-type (WT). Red: <i>∆mutant</i>. NT: no treatment. TOB: growth in the presence of tobramycin at 20% of the MIC. Reporters described in Fabret and Namy, 2021, and Fruchard et al 2025. Y-axis represents stop codon readthrough*1000. Number of biological replicates: betwe...

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Autore principale:	Léo Hardy (22538036) (author)
Altri autori:	Virginie Marchand (219509) (author), Valérie Bourguignon (14503342) (author), Quentin Thuillier (22538039) (author), Cathy Dias (22538042) (author), Evelyne Krin (252784) (author), Louna Fruchard (22538045) (author), Dan Bar Yaacov (11965433) (author), Didier Mazel (158958) (author), Yuri Motorin (773232) (author), Zeynep Baharoglu (88371) (author)
Pubblicazione:	2025
Soggetti:	Biophysics Biochemistry Microbiology Genetics Molecular Biology Sociology Space Science Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified subinhibitory antibiotic stress first experimentally validated ensuring precise decoding div >< p trna epitranscriptomic landscape functional trmk enzyme based sequencing methods vibrio cholerae </ comprehensive modification landscape rna modification enzymes cholerae </ functional impact cognate enzymes coli </ trna modifications work provides translation regulation transfer rnas standard conditions specific features specific differences rna species rna modifications protein synthesis positive bacteria occurs independently largely restricted human pathogen genetic code future research evaluated fitness diversely modified delivering aminoacids deletion strains comparing wild comparative analysis biological significance biological importance anticodon stem
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Stop codon readthrough for tRNA modification gene deletion mutants.

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