Prevalence of <i>adeB</i> and <i>adeR</i> in <i>Acinetobacter</i> spp.
<div><p><i>Acinetobacter baumannii</i> and <i>Enterobacter cloacae</i> are phylogenetically distant Gram−negative bacterial pathogens that represent significant challenges in healthcare settings due to their remarkable ability to acquire antimicrobial resistance....
محفوظ في:
| المؤلف الرئيسي: | |
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| مؤلفون آخرون: | , , , , , |
| منشور في: |
2025
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| الموضوعات: | |
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| _version_ | 1852022221139083264 |
|---|---|
| author | Marc Gaona (20854802) |
| author2 | Jordi Corral (8680446) Miquel Sánchez−Osuna (20854805) Susana Campoy (13473) Jordi Barbé (13475) María Pérez-Varela (8680449) Jesús Aranda (309520) |
| author2_role | author author author author author author |
| author_facet | Marc Gaona (20854802) Jordi Corral (8680446) Miquel Sánchez−Osuna (20854805) Susana Campoy (13473) Jordi Barbé (13475) María Pérez-Varela (8680449) Jesús Aranda (309520) |
| author_role | author |
| dc.creator.none.fl_str_mv | Marc Gaona (20854802) Jordi Corral (8680446) Miquel Sánchez−Osuna (20854805) Susana Campoy (13473) Jordi Barbé (13475) María Pérez-Varela (8680449) Jesús Aranda (309520) |
| dc.date.none.fl_str_mv | 2025-03-10T17:30:33Z |
| dc.identifier.none.fl_str_mv | 10.1371/journal.pone.0315428.s001 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/dataset/Prevalence_of_i_adeB_i_and_i_adeR_i_in_i_Acinetobacter_i_spp_/28567472 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biochemistry Microbiology Cell Biology Genetics Molecular Biology Evolutionary Biology Infectious Diseases Virology Computational Biology Biological Sciences not elsewhere classified study investigates one similar dna motifs open new perspectives identifies homologous components healthcare settings due common ancestor rather alters colistin susceptibility various antimicrobial classes acquire antimicrobial resistance represent significant challenges adeb pump component enterobacter cloacae </ acinetobacter baumannii </ antimicrobial resistance cloacae </ baumannii </ vivo </ vitro </ silico </ adeb </ ader </ work highlight restored interchangeably remarkable ability reciprocal regulation promoter regions previously published phylogenomic analysis phylogenetic trees likely derived knockout mutant genes suggest gene encoding functionally substitute ancestral states affected phenotypes adeb protein |
| dc.title.none.fl_str_mv | Prevalence of <i>adeB</i> and <i>adeR</i> in <i>Acinetobacter</i> spp. |
| dc.type.none.fl_str_mv | Dataset info:eu-repo/semantics/publishedVersion dataset |
| description | <div><p><i>Acinetobacter baumannii</i> and <i>Enterobacter cloacae</i> are phylogenetically distant Gram−negative bacterial pathogens that represent significant challenges in healthcare settings due to their remarkable ability to acquire antimicrobial resistance. This study investigates one of the most important efflux pump systems in <i>A. baumannii</i>, AdeABC−AdeRS, and identifies homologous components in <i>E. cloacae</i>. By constructing isogenic knockout mutants, we show that the AdeB pump component and the AdeR regulator are significant for antimicrobial resistance and pathogenicity in <i>A. baumannii</i>. Through <i>in silico</i> predictions, we identify homologs of AdeB and AdeR (ECL_01758 and ECL_01761, respectively) in <i>E. cloacae</i>. Notably, we demonstrate that while the inactivation of the <i>E. cloacae</i> gene encoding the AdeB protein does not impact on pathogenesis and only alters colistin susceptibility, a knockout mutant of the gene encoding the AdeR regulator significantly affects susceptibility to various antimicrobial classes, motility, and virulence. Additionally, we demonstrate that the AdeR regulators <i>of A. baumannii</i> and <i>E. cloacae</i> can functionally substitute for each other both <i>in vitro</i> and <i>in vivo</i> conditions. Electrophoretic mobility shift assays reveal that these regulators are capable of binding to the promoter regions of each other’s species, where similar DNA motifs are present. Furthermore, cross−complementation tests show that the affected phenotypes in each species can be restored interchangeably. Moreover, phylogenomic analysis of previously published <i>E.cloacae</i> genomes and reconstructrion of ancestral states through the phylogenetic trees of the <i>adeB</i> and <i>adeR</i> genes suggest that these homologs are more likely derived from a common ancestor rather than through recent horizontal gene transfer. The findings of this work highlight that conserved regulatory functions concerning efflux pump expression can be maintained across species despite evolutionary divergence and open new perspectives for the control of bacterial infections.</p></div> |
| eu_rights_str_mv | openAccess |
| id | Manara_b3e2dffad19bc1007201fdddf7fd8a9e |
| identifier_str_mv | 10.1371/journal.pone.0315428.s001 |
| network_acronym_str | Manara |
| network_name_str | ManaraRepo |
| oai_identifier_str | oai:figshare.com:article/28567472 |
| publishDate | 2025 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Prevalence of <i>adeB</i> and <i>adeR</i> in <i>Acinetobacter</i> spp. Marc Gaona (20854802)Jordi Corral (8680446)Miquel Sánchez−Osuna (20854805)Susana Campoy (13473)Jordi Barbé (13475)María Pérez-Varela (8680449)Jesús Aranda (309520)BiochemistryMicrobiologyCell BiologyGeneticsMolecular BiologyEvolutionary BiologyInfectious DiseasesVirologyComputational BiologyBiological Sciences not elsewhere classifiedstudy investigates onesimilar dna motifsopen new perspectivesidentifies homologous componentshealthcare settings duecommon ancestor ratheralters colistin susceptibilityvarious antimicrobial classesacquire antimicrobial resistancerepresent significant challengesadeb pump componententerobacter cloacae </acinetobacter baumannii </antimicrobial resistancecloacae </baumannii </vivo </vitro </silico </adeb </ader </work highlightrestored interchangeablyremarkable abilityreciprocal regulationpromoter regionspreviously publishedphylogenomic analysisphylogenetic treeslikely derivedknockout mutantgenes suggestgene encodingfunctionally substituteancestral statesaffected phenotypesadeb protein<div><p><i>Acinetobacter baumannii</i> and <i>Enterobacter cloacae</i> are phylogenetically distant Gram−negative bacterial pathogens that represent significant challenges in healthcare settings due to their remarkable ability to acquire antimicrobial resistance. This study investigates one of the most important efflux pump systems in <i>A. baumannii</i>, AdeABC−AdeRS, and identifies homologous components in <i>E. cloacae</i>. By constructing isogenic knockout mutants, we show that the AdeB pump component and the AdeR regulator are significant for antimicrobial resistance and pathogenicity in <i>A. baumannii</i>. Through <i>in silico</i> predictions, we identify homologs of AdeB and AdeR (ECL_01758 and ECL_01761, respectively) in <i>E. cloacae</i>. Notably, we demonstrate that while the inactivation of the <i>E. cloacae</i> gene encoding the AdeB protein does not impact on pathogenesis and only alters colistin susceptibility, a knockout mutant of the gene encoding the AdeR regulator significantly affects susceptibility to various antimicrobial classes, motility, and virulence. Additionally, we demonstrate that the AdeR regulators <i>of A. baumannii</i> and <i>E. cloacae</i> can functionally substitute for each other both <i>in vitro</i> and <i>in vivo</i> conditions. Electrophoretic mobility shift assays reveal that these regulators are capable of binding to the promoter regions of each other’s species, where similar DNA motifs are present. Furthermore, cross−complementation tests show that the affected phenotypes in each species can be restored interchangeably. Moreover, phylogenomic analysis of previously published <i>E.cloacae</i> genomes and reconstructrion of ancestral states through the phylogenetic trees of the <i>adeB</i> and <i>adeR</i> genes suggest that these homologs are more likely derived from a common ancestor rather than through recent horizontal gene transfer. The findings of this work highlight that conserved regulatory functions concerning efflux pump expression can be maintained across species despite evolutionary divergence and open new perspectives for the control of bacterial infections.</p></div>2025-03-10T17:30:33ZDatasetinfo:eu-repo/semantics/publishedVersiondataset10.1371/journal.pone.0315428.s001https://figshare.com/articles/dataset/Prevalence_of_i_adeB_i_and_i_adeR_i_in_i_Acinetobacter_i_spp_/28567472CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/285674722025-03-10T17:30:33Z |
| spellingShingle | Prevalence of <i>adeB</i> and <i>adeR</i> in <i>Acinetobacter</i> spp. Marc Gaona (20854802) Biochemistry Microbiology Cell Biology Genetics Molecular Biology Evolutionary Biology Infectious Diseases Virology Computational Biology Biological Sciences not elsewhere classified study investigates one similar dna motifs open new perspectives identifies homologous components healthcare settings due common ancestor rather alters colistin susceptibility various antimicrobial classes acquire antimicrobial resistance represent significant challenges adeb pump component enterobacter cloacae </ acinetobacter baumannii </ antimicrobial resistance cloacae </ baumannii </ vivo </ vitro </ silico </ adeb </ ader </ work highlight restored interchangeably remarkable ability reciprocal regulation promoter regions previously published phylogenomic analysis phylogenetic trees likely derived knockout mutant genes suggest gene encoding functionally substitute ancestral states affected phenotypes adeb protein |
| status_str | publishedVersion |
| title | Prevalence of <i>adeB</i> and <i>adeR</i> in <i>Acinetobacter</i> spp. |
| title_full | Prevalence of <i>adeB</i> and <i>adeR</i> in <i>Acinetobacter</i> spp. |
| title_fullStr | Prevalence of <i>adeB</i> and <i>adeR</i> in <i>Acinetobacter</i> spp. |
| title_full_unstemmed | Prevalence of <i>adeB</i> and <i>adeR</i> in <i>Acinetobacter</i> spp. |
| title_short | Prevalence of <i>adeB</i> and <i>adeR</i> in <i>Acinetobacter</i> spp. |
| title_sort | Prevalence of <i>adeB</i> and <i>adeR</i> in <i>Acinetobacter</i> spp. |
| topic | Biochemistry Microbiology Cell Biology Genetics Molecular Biology Evolutionary Biology Infectious Diseases Virology Computational Biology Biological Sciences not elsewhere classified study investigates one similar dna motifs open new perspectives identifies homologous components healthcare settings due common ancestor rather alters colistin susceptibility various antimicrobial classes acquire antimicrobial resistance represent significant challenges adeb pump component enterobacter cloacae </ acinetobacter baumannii </ antimicrobial resistance cloacae </ baumannii </ vivo </ vitro </ silico </ adeb </ ader </ work highlight restored interchangeably remarkable ability reciprocal regulation promoter regions previously published phylogenomic analysis phylogenetic trees likely derived knockout mutant genes suggest gene encoding functionally substitute ancestral states affected phenotypes adeb protein |