Effect of IDR deletion on stress tolerance, DON production and sexual/asexual development of <i>F. graminearum.</i>
<p>(A) The colony growth of <i>F. graminearum</i> mutants lacking different IDR sequences compared to PH-1. The strains were cultured on CM agar plates for 3 days at 28°C. (B) Graphical representation of the growth inhibition rates of the different IDR mutants due to the various st...
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| _version_ | 1849927641189056512 |
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| author | Yakubu Saddeeq Abubakar (5558399) |
| author2 | Shiqi Ji (20476509) Qiaoli Chen (538344) Huawei Zheng (713784) Zonghua Wang (133934) Wenhui Zheng (133904) Yi Lou (381696) |
| author2_role | author author author author author author |
| author_facet | Yakubu Saddeeq Abubakar (5558399) Shiqi Ji (20476509) Qiaoli Chen (538344) Huawei Zheng (713784) Zonghua Wang (133934) Wenhui Zheng (133904) Yi Lou (381696) |
| author_role | author |
| dc.creator.none.fl_str_mv | Yakubu Saddeeq Abubakar (5558399) Shiqi Ji (20476509) Qiaoli Chen (538344) Huawei Zheng (713784) Zonghua Wang (133934) Wenhui Zheng (133904) Yi Lou (381696) |
| dc.date.none.fl_str_mv | 2025-11-24T18:35:59Z |
| dc.identifier.none.fl_str_mv | 10.1371/journal.ppat.1013726.s016 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/figure/Effect_of_IDR_deletion_on_stress_tolerance_DON_production_and_sexual_asexual_development_of_i_F_graminearum_i_/30697850 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biophysics Biochemistry Medicine Microbiology Cell Biology Genetics Developmental Biology Infectious Diseases Plant Biology div >< p conserved molecular scaffolds irregular colony edges form protein condensates dynamic protein assembly remaining 20 proteins plant fungal pathogen idrs largely altered proteins exhibited features fusarium graminearum </ graminearum </ fusarium </ protein interaction plant infection mediate protein irregular septation fungal pathogenesis containing proteins wide characterization varied functions systematically identified src homology septal pore previously characterized potential targets plasma membrane nuclear fragmentation nuclear distribution negative regulators mutant strains multifaceted functions hyphal tip head blight functional diversity completely abolished apical collar |
| dc.title.none.fl_str_mv | Effect of IDR deletion on stress tolerance, DON production and sexual/asexual development of <i>F. graminearum.</i> |
| dc.type.none.fl_str_mv | Image Figure info:eu-repo/semantics/publishedVersion image |
| description | <p>(A) The colony growth of <i>F. graminearum</i> mutants lacking different IDR sequences compared to PH-1. The strains were cultured on CM agar plates for 3 days at 28°C. (B) Graphical representation of the growth inhibition rates of the different IDR mutants due to the various stress factors. The mutants displayed varying degree of stress tolerance. The values presented are means ± SD from three independent experiments. Bars marked with black asterisks are significantly more inhibited than PH-1, while those marked with red asterisks are significantly less inhibited than PH-1 (*, p < 0.05). (C) Deletion of IDRs in SH3 domain-containing proteins does not influence DON production in <i>F. graminearum</i>. The values presented are means ± SD from three independent experiments run in duplicate. ns: non-significant. (D) Deletion of IDR1 (but not IDR2) of FgPin3 compromises sexual development in <i>F. graminearum</i>. The strains were cultured on carrot agar (CA) plates and exposed to near-UV light for 14 days at 19°C. Bar = 500 μm. The experiment was conducted in triplicate. (E) Deletion of IDRs in SH3 domain-containing proteins does not influence conidiation in <i>F. graminearum</i>. The values presented represent mean ± SD. Each strain was counted five times. ns: non-significant.</p> <p>(TIF)</p> |
| eu_rights_str_mv | openAccess |
| id | Manara_b3e4bd609a9445c076746ccecded8946 |
| identifier_str_mv | 10.1371/journal.ppat.1013726.s016 |
| network_acronym_str | Manara |
| network_name_str | ManaraRepo |
| oai_identifier_str | oai:figshare.com:article/30697850 |
| publishDate | 2025 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Effect of IDR deletion on stress tolerance, DON production and sexual/asexual development of <i>F. graminearum.</i>Yakubu Saddeeq Abubakar (5558399)Shiqi Ji (20476509)Qiaoli Chen (538344)Huawei Zheng (713784)Zonghua Wang (133934)Wenhui Zheng (133904)Yi Lou (381696)BiophysicsBiochemistryMedicineMicrobiologyCell BiologyGeneticsDevelopmental BiologyInfectious DiseasesPlant Biologydiv >< pconserved molecular scaffoldsirregular colony edgesform protein condensatesdynamic protein assemblyremaining 20 proteinsplant fungal pathogenidrs largely alteredproteins exhibited featuresfusarium graminearum </graminearum </fusarium </protein interactionplant infectionmediate proteinirregular septationfungal pathogenesiscontaining proteinswide characterizationvaried functionssystematically identifiedsrc homologyseptal porepreviously characterizedpotential targetsplasma membranenuclear fragmentationnuclear distributionnegative regulatorsmutant strainsmultifaceted functionshyphal tiphead blightfunctional diversitycompletely abolishedapical collar<p>(A) The colony growth of <i>F. graminearum</i> mutants lacking different IDR sequences compared to PH-1. The strains were cultured on CM agar plates for 3 days at 28°C. (B) Graphical representation of the growth inhibition rates of the different IDR mutants due to the various stress factors. The mutants displayed varying degree of stress tolerance. The values presented are means ± SD from three independent experiments. Bars marked with black asterisks are significantly more inhibited than PH-1, while those marked with red asterisks are significantly less inhibited than PH-1 (*, p < 0.05). (C) Deletion of IDRs in SH3 domain-containing proteins does not influence DON production in <i>F. graminearum</i>. The values presented are means ± SD from three independent experiments run in duplicate. ns: non-significant. (D) Deletion of IDR1 (but not IDR2) of FgPin3 compromises sexual development in <i>F. graminearum</i>. The strains were cultured on carrot agar (CA) plates and exposed to near-UV light for 14 days at 19°C. Bar = 500 μm. The experiment was conducted in triplicate. (E) Deletion of IDRs in SH3 domain-containing proteins does not influence conidiation in <i>F. graminearum</i>. The values presented represent mean ± SD. Each strain was counted five times. ns: non-significant.</p> <p>(TIF)</p>2025-11-24T18:35:59ZImageFigureinfo:eu-repo/semantics/publishedVersionimage10.1371/journal.ppat.1013726.s016https://figshare.com/articles/figure/Effect_of_IDR_deletion_on_stress_tolerance_DON_production_and_sexual_asexual_development_of_i_F_graminearum_i_/30697850CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/306978502025-11-24T18:35:59Z |
| spellingShingle | Effect of IDR deletion on stress tolerance, DON production and sexual/asexual development of <i>F. graminearum.</i> Yakubu Saddeeq Abubakar (5558399) Biophysics Biochemistry Medicine Microbiology Cell Biology Genetics Developmental Biology Infectious Diseases Plant Biology div >< p conserved molecular scaffolds irregular colony edges form protein condensates dynamic protein assembly remaining 20 proteins plant fungal pathogen idrs largely altered proteins exhibited features fusarium graminearum </ graminearum </ fusarium </ protein interaction plant infection mediate protein irregular septation fungal pathogenesis containing proteins wide characterization varied functions systematically identified src homology septal pore previously characterized potential targets plasma membrane nuclear fragmentation nuclear distribution negative regulators mutant strains multifaceted functions hyphal tip head blight functional diversity completely abolished apical collar |
| status_str | publishedVersion |
| title | Effect of IDR deletion on stress tolerance, DON production and sexual/asexual development of <i>F. graminearum.</i> |
| title_full | Effect of IDR deletion on stress tolerance, DON production and sexual/asexual development of <i>F. graminearum.</i> |
| title_fullStr | Effect of IDR deletion on stress tolerance, DON production and sexual/asexual development of <i>F. graminearum.</i> |
| title_full_unstemmed | Effect of IDR deletion on stress tolerance, DON production and sexual/asexual development of <i>F. graminearum.</i> |
| title_short | Effect of IDR deletion on stress tolerance, DON production and sexual/asexual development of <i>F. graminearum.</i> |
| title_sort | Effect of IDR deletion on stress tolerance, DON production and sexual/asexual development of <i>F. graminearum.</i> |
| topic | Biophysics Biochemistry Medicine Microbiology Cell Biology Genetics Developmental Biology Infectious Diseases Plant Biology div >< p conserved molecular scaffolds irregular colony edges form protein condensates dynamic protein assembly remaining 20 proteins plant fungal pathogen idrs largely altered proteins exhibited features fusarium graminearum </ graminearum </ fusarium </ protein interaction plant infection mediate protein irregular septation fungal pathogenesis containing proteins wide characterization varied functions systematically identified src homology septal pore previously characterized potential targets plasma membrane nuclear fragmentation nuclear distribution negative regulators mutant strains multifaceted functions hyphal tip head blight functional diversity completely abolished apical collar |