Effect of IDR deletion on stress tolerance, DON production and sexual/asexual development of <i>F. graminearum.</i>

Effect of IDR deletion on stress tolerance, DON production and sexual/asexual development of <i>F. graminearum.</i>

<p>(A) The colony growth of <i>F. graminearum</i> mutants lacking different IDR sequences compared to PH-1. The strains were cultured on CM agar plates for 3 days at 28°C. (B) Graphical representation of the growth inhibition rates of the different IDR mutants due to the various st...

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Номзүйн дэлгэрэнгүй
Үндсэн зохиолч:	Yakubu Saddeeq Abubakar (5558399) (author)
Бусад зохиолчид:	Shiqi Ji (20476509) (author), Qiaoli Chen (538344) (author), Huawei Zheng (713784) (author), Zonghua Wang (133934) (author), Wenhui Zheng (133904) (author), Yi Lou (381696) (author)
Хэвлэсэн:	2025
Нөхцлүүд:	Biophysics Biochemistry Medicine Microbiology Cell Biology Genetics Developmental Biology Infectious Diseases Plant Biology div >< p conserved molecular scaffolds irregular colony edges form protein condensates dynamic protein assembly remaining 20 proteins plant fungal pathogen idrs largely altered proteins exhibited features fusarium graminearum </ graminearum </ fusarium </ protein interaction plant infection mediate protein irregular septation fungal pathogenesis containing proteins wide characterization varied functions systematically identified src homology septal pore previously characterized potential targets plasma membrane nuclear fragmentation nuclear distribution negative regulators mutant strains multifaceted functions hyphal tip head blight functional diversity completely abolished apical collar
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Ижил төстэй зүйлс

Role of SH3 domain-containing proteins on deoxynivalenol (DON) production in <i>F. graminearum.</i>
-н: Yakubu Saddeeq Abubakar (5558399)
Хэвлэсэн: (2025)

Influence of SH3 domain-containing proteins on sexual development of <i>F. graminearum.</i>
-н: Yakubu Saddeeq Abubakar (5558399)
Хэвлэсэн: (2025)

Effect of SH3 domain gene deletions on the pathogenicity of <i>F. graminearum.</i>
-н: Yakubu Saddeeq Abubakar (5558399)
Хэвлэсэн: (2025)

PCR gels showing successful deletion of SH3 genes in <i>F. graminearum.</i>
-н: Yakubu Saddeeq Abubakar (5558399)
Хэвлэсэн: (2025)

<i>In silico</i> analysis of intrinsically disordered sequences (IDRs) in the SH3 domain-containing proteins in <i>F. graminearum.</i>
-н: Yakubu Saddeeq Abubakar (5558399)
Хэвлэсэн: (2025)

qRT-PCR analyses confirming the deletion of the SH3 domain-containing genes in <i>F. graminearum.</i>
-н: Yakubu Saddeeq Abubakar (5558399)
Хэвлэсэн: (2025)

Effects of <i>FgHOF1</i> deletion on cytoskeletal and nuclear distribution.
-н: Yakubu Saddeeq Abubakar (5558399)
Хэвлэсэн: (2025)

qRT-PCR showing the expression of sexual reproduction-responsive genes in Δ<i>Fghof1</i> and Δ<i>Fgrax2</i> mutants.
-н: Yakubu Saddeeq Abubakar (5558399)
Хэвлэсэн: (2025)

Subcellular localizations of SH3 domain-containing proteins in <i>F. graminearum</i> hyphae.
-н: Yakubu Saddeeq Abubakar (5558399)
Хэвлэсэн: (2025)

Impact of SH3 domain-containing proteins on the vegetative growth of <i>F. graminearum.</i>
-н: Yakubu Saddeeq Abubakar (5558399)
Хэвлэсэн: (2025)

SH3 domain-containing genes and the subcellular localization of their respective products in <i>F. graminearum.</i>
-н: Yakubu Saddeeq Abubakar (5558399)
Хэвлэсэн: (2025)

SH3 domain-containing proteins exhibit features of phase separation in <i>F. graminearum.</i>
-н: Yakubu Saddeeq Abubakar (5558399)
Хэвлэсэн: (2025)

The IDRs in SH3 domain-containing proteins are important for the fusion of cytoplasmic protein condensates.
-н: Yakubu Saddeeq Abubakar (5558399)
Хэвлэсэн: (2025)

The file compiles all original, uncropped, and unprocessed data collected in the experiments, including colony diameter, conidia production, conidia size, septa of conidia, DON production, the disease indexes, relative gene expression level.
-н: Yakubu Saddeeq Abubakar (5558399)
Хэвлэсэн: (2025)

Time-lapse images showing the fusion of two FgRvs167<sup>ΔIDR</sup>-GFP protein condensates in young mycelia.
-н: Yakubu Saddeeq Abubakar (5558399)
Хэвлэсэн: (2025)

Time-lapse images showing the fusion of two FgPin<sup>ΔIDR1</sup>-GFP protein condensates in young mycelia.
-н: Yakubu Saddeeq Abubakar (5558399)
Хэвлэсэн: (2025)

Time-lapse images showing the fusion of two FgPin3<sup>ΔIDR2</sup>-GFP protein condensates in young mycelia.
-н: Yakubu Saddeeq Abubakar (5558399)
Хэвлэсэн: (2025)

Live-cell images of fluorescence recovery after photobleaching (FRAP) experiments in the strain expressing FgRvs167<sup>ΔIDR</sup>-GFP.
-н: Yakubu Saddeeq Abubakar (5558399)
Хэвлэсэн: (2025)

Live-cell images of fluorescence recovery after photobleaching (FRAP) experiments in the strain expressing FgPin3<sup>ΔIDR2</sup>-GFP.
-н: Yakubu Saddeeq Abubakar (5558399)
Хэвлэсэн: (2025)

Live-cell images of fluorescence recovery after photobleaching (FRAP) experiments in the strain expressing FgPin3<sup>ΔIDR1</sup>-GFP.
-н: Yakubu Saddeeq Abubakar (5558399)
Хэвлэсэн: (2025)

Defects of Δ<i>Fgcyk3</i> mutant in nuclear division.
-н: Yakubu Saddeeq Abubakar (5558399)
Хэвлэсэн: (2025)

Δ<i>Fghof1</i> mutant could be compromised in cell division.
-н: Yakubu Saddeeq Abubakar (5558399)
Хэвлэсэн: (2025)

Sensitivity of Δ<i>Fghof1</i> mutant conidia to different DAPI concentrations.
-н: Yakubu Saddeeq Abubakar (5558399)
Хэвлэсэн: (2025)

Abnormal Δ<i>Fghof1</i> mutant conidia also exhibit nuclear fragmentation.
-н: Yakubu Saddeeq Abubakar (5558399)
Хэвлэсэн: (2025)

Mobility of FgAip5-GFP and FM4–64 in the hyphal tip of Δ<i>Fgaip5-</i>Com.
-н: Yakubu Saddeeq Abubakar (5558399)
Хэвлэсэн: (2025)

Summary of the general phenotype of SH3 domain-containing gene mutants.
-н: Yakubu Saddeeq Abubakar (5558399)
Хэвлэсэн: (2025)

Co-localization of FgAip5-GFP at hyphal tips and various SH3-domain proteins at membrane-associated structures.
-н: Yakubu Saddeeq Abubakar (5558399)
Хэвлэсэн: (2025)

Conidiation and conidial morphology of the SH3 domain-containing gene mutants.
-н: Yakubu Saddeeq Abubakar (5558399)
Хэвлэсэн: (2025)

Time-lapse images showing the fusion of two FgBoi1-GFP protein condensates in young mycelia.
-н: Yakubu Saddeeq Abubakar (5558399)
Хэвлэсэн: (2025)

Time-lapse images showing the fusion of two FgRvs167-GFP protein condensates in young mycelia.
-н: Yakubu Saddeeq Abubakar (5558399)
Хэвлэсэн: (2025)

Time-lapse images showing the fusion of two FgPin3-GFP protein condensates in young mycelia.
-н: Yakubu Saddeeq Abubakar (5558399)
Хэвлэсэн: (2025)

Live-cell images of fluorescence recovery after photobleaching (FRAP) experiments in the strain expressing FgBoi1-GFP.
-н: Yakubu Saddeeq Abubakar (5558399)
Хэвлэсэн: (2025)

Live-cell images of fluorescence recovery after photobleaching (FRAP) experiments in the strain expressing FgRvs167-GFP.
-н: Yakubu Saddeeq Abubakar (5558399)
Хэвлэсэн: (2025)

Live-cell images of fluorescence recovery after photobleaching (FRAP) experiments in the strain expressing FgPin3-GFP.
-н: Yakubu Saddeeq Abubakar (5558399)
Хэвлэсэн: (2025)

FRAP and protein fusion analyses in FgBoi1-GFP.
-н: Yakubu Saddeeq Abubakar (5558399)
Хэвлэсэн: (2025)

Sensitivity of the SH3 domain-containing gene mutants to various stress-inducing agents.
-н: Yakubu Saddeeq Abubakar (5558399)
Хэвлэсэн: (2025)

Phylogenetic analysis of the 29 SH3 domain-containing proteins across different fungal species.
-н: Yakubu Saddeeq Abubakar (5558399)
Хэвлэсэн: (2025)

Localization of the CTC to the mitochondrion of <i>Toxoplasma gondii</i> is mediated by <i>Tg</i>CIA1.
-н: Evie R. Hodgson (21866946)
Хэвлэсэн: (2025)

Epitope tagging <i>Tg</i>ABCE1 in the r<i>Tg</i>CIA1-mAID-HA and r<i>Tg</i>Dre2-mAID-HA lines.
-н: Evie R. Hodgson (21866946)
Хэвлэсэн: (2025)

An elongated CD5 loop is necessary for targeting <i>Tg</i>CIA1 to the mitochondrion of <i>Toxoplasma gondii.</i>
-н: Evie R. Hodgson (21866946)
Хэвлэсэн: (2025)

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