<i>Dancr</i> regulates gene expression pathways involved in cell death.
<p>CRISPR-Cas9 mediated deletion of the zebrafish <i>dancr</i> promoter leads to a reduction in <i>dancr</i>, <i>snora26</i> and <i>snora27</i> expression. <b>(A)</b> Integrative Genomics Viewer visualisation of RNA-seq reads mapping...
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2025
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| Summary: | <p>CRISPR-Cas9 mediated deletion of the zebrafish <i>dancr</i> promoter leads to a reduction in <i>dancr</i>, <i>snora26</i> and <i>snora27</i> expression. <b>(A)</b> Integrative Genomics Viewer visualisation of RNA-seq reads mapping to the <i>dancr</i> locus in mock control and F0 dancr crispant embryos at 30 hpf. The locations of the four guide RNAs targeting the <i>dancr</i> promoter and primers used for RT-qPCR are shown. <b>(B)</b> <i>Dancr, snora26</i> and <i>snora27</i> expression levels were measured in F0 dancr crispant and mock control embryos at 30 hpf using RT-qPCR. <i>actb2</i> was used as a reference gene. Results presented as mean ± sem, n = 3. Two-tailed two sample t-test p < 0.01**. Individual dots represent separate biological replicates. <b>(C, D)</b> <i>Dancr</i> regulates gene pathways involved in cell death and the immune response in the developing zebrafish embryo. <b>(C)</b> <i>Dancr</i> promoter deletion induces statistically significant changes in the expression of 164 genes (DESeq2 padj<=0.05; log2FoldChange>=1.0). <b>(D)</b> KEGG pathway analysis of the <i>dancr</i> regulated gene set identified significantly enriched pathways (FDR 5%). The number of genes found in each category are indicated.</p> |
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