TAK1 activation induces RORγ expression to enhance cholesterol biosynthesis.

<p>(A and B) NL20 cells were transfected with the empty vector or the vector encoding TAK1. After incubation for 48 h, the cells were infected with the indicated MOIs of H5N1 and incubated for 16 h. Cell lysates were analyzed for the levels of the indicated proteins (A). Conditioned media were...

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التفاصيل البيبلوغرافية
المؤلف الرئيسي:	Jingting Zhang (791900) (author)
مؤلفون آخرون:	Ruixuan Cao (22493337) (author), Yujie Wang (308942) (author), Yuling Sun (5688665) (author), Xiaoyue Ji (14272545) (author), Penggang Liu (2512177) (author), Kaituo Liu (10512226) (author), Jing Sun (141206) (author), Xiaojun Chen (317104) (author), Demin Cai (621171) (author), Pinghu Zhang (4029842) (author), Xiaoquan Wang (305826) (author), Xiufan Liu (26081) (author), Xiulong Xu (342383) (author)
منشور في:	2025
الموضوعات:	Biochemistry Microbiology Cell Biology Genetics Molecular Biology Immunology Cancer Science Policy Mental Health Infectious Diseases Plant Biology Virology xlink "> infection turn activate ap1 supply substantial amounts sterol regulatory element orphan nuclear receptor ror &# 947 facilitate &# 160 nf -&# 954 exogenous cholesterol rescues iav infection induces axis </ p induced hmgcr expression xy018 treatment reduces facilitate virus replication &# 954 hmgcr ), cholesterol biosynthesis cellular cholesterol viral replication terminal kinase suppress iav retinoic acid prolongs survival methylglutaryl coenzyme key enzyme jun n inhibitory effect infected mice ikk ), downstream kinases
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الملخص:	<p>(A and B) NL20 cells were transfected with the empty vector or the vector encoding TAK1. After incubation for 48 h, the cells were infected with the indicated MOIs of H5N1 and incubated for 16 h. Cell lysates were analyzed for the levels of the indicated proteins (A). Conditioned media were collected and analyzed for virus titers by measuring the TCID<sub>50</sub> assay (B). Data represents the mean ± SD of three independent experiments. <i><i>p</i></i> < 0.05, <i><i>p</i></i> < 0.01. (C and D) Control and TAK1-deficient NL20 cells infected with the indicated MOIs of H5N1 (SY) were incubated for 16 h. Cell lysates were prepared and analyzed for the expression of cholesterol biosynthesis-related and viral proteins (C). Conditioned medial were collected and analyzed for virus titers by measuring TCID<sub>50</sub> values (D). △TAK1, TAK1 deficiency. Data are the mean ± SD of three experiments. <i><i>p</i></i> < 0.05, *<i><i>p</i></i> < 0.01. (E) Total cellular RNAs from control and TAK1-deficient cells were extracted and analyzed for the levels of <i>RORC</i> and <i>HMGCR</i> mRNA levels by RT-qPCR. Data represents the mean ± SD of three independent experiments. <i><i>p</i></i> < 0.05, **<i><i>p</i></i> < 0.01. (F-G) TAK1-deficient NL20 cells were infected with higher MOIs of H5N1 (SY) than control NL20 cells. After incubation for 16 h, cell lysates were analyzed for the expression of cholesterol biosynthesis-related and viral proteins (F). Conditioned medial were collected and analyzed for virus titers by measuring TCID<sub>50</sub> values (G). Data are the mean ± SD of three experiments. ns, non-significant.</p>

TAK1 activation induces RORγ expression to enhance cholesterol biosynthesis.

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