Image 4_Prediction of prognosis, immunogenicity and efficacy of immunotherapy based on cholesterol metabolism in gastric cancer.jpeg

Background<p>Cholesterol metabolism plays a crucial role in tumor progression and immune response modulation. However, the precise connection between cholesterol metabolism-related genes (CMRGs) and their implications for clinical prognosis, the tumor microenvironment (TME), and the outcomes o...

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Main Author: Chengjun Zhu (3948320) (author)
Other Authors: Mengpei Yan (20470484) (author), Zhijun Zhang (315079) (author), Yikai Shen (17017618) (author), Wangwen Wang (20470487) (author), Zetian Chen (8586345) (author), Changsheng Cai (20470490) (author), Hongda Liu (6702581) (author), Zekuan Xu (308992) (author), Zheng Li (26302) (author)
Published: 2024
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_version_ 1852024144708763648
author Chengjun Zhu (3948320)
author2 Mengpei Yan (20470484)
Zhijun Zhang (315079)
Yikai Shen (17017618)
Wangwen Wang (20470487)
Zetian Chen (8586345)
Changsheng Cai (20470490)
Hongda Liu (6702581)
Zekuan Xu (308992)
Zheng Li (26302)
author2_role author
author
author
author
author
author
author
author
author
author_facet Chengjun Zhu (3948320)
Mengpei Yan (20470484)
Zhijun Zhang (315079)
Yikai Shen (17017618)
Wangwen Wang (20470487)
Zetian Chen (8586345)
Changsheng Cai (20470490)
Hongda Liu (6702581)
Zekuan Xu (308992)
Zheng Li (26302)
author_role author
dc.creator.none.fl_str_mv Chengjun Zhu (3948320)
Mengpei Yan (20470484)
Zhijun Zhang (315079)
Yikai Shen (17017618)
Wangwen Wang (20470487)
Zetian Chen (8586345)
Changsheng Cai (20470490)
Hongda Liu (6702581)
Zekuan Xu (308992)
Zheng Li (26302)
dc.date.none.fl_str_mv 2024-12-24T05:07:40Z
dc.identifier.none.fl_str_mv 10.3389/fonc.2024.1518010.s005
dc.relation.none.fl_str_mv https://figshare.com/articles/figure/Image_4_Prediction_of_prognosis_immunogenicity_and_efficacy_of_immunotherapy_based_on_cholesterol_metabolism_in_gastric_cancer_jpeg/28088252
dc.rights.none.fl_str_mv CC BY 4.0
info:eu-repo/semantics/openAccess
dc.subject.none.fl_str_mv Oncology and Carcinogenesis not elsewhere classified
gastric cancer
cholesterol
prognosis
tumor microenvironment
immunotherapy
dc.title.none.fl_str_mv Image 4_Prediction of prognosis, immunogenicity and efficacy of immunotherapy based on cholesterol metabolism in gastric cancer.jpeg
dc.type.none.fl_str_mv Image
Figure
info:eu-repo/semantics/publishedVersion
image
description Background<p>Cholesterol metabolism plays a crucial role in tumor progression and immune response modulation. However, the precise connection between cholesterol metabolism-related genes (CMRGs) and their implications for clinical prognosis, the tumor microenvironment (TME), and the outcomes of immunotherapy in gastric cancer remains to be fully elucidated.</p>Methods<p>Transcriptome data and related clinical information from 675 gastric cancer patients were downloaded from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases. A total of 50 cholesterol metabolism-related genes (CMRGs) were identified from the Kyoto Encyclopedia of Genes and Genomes (KEGG, hsa04979). Consensus clustering analysis was used to classify patients into distinct molecular subgroups, while principal component analysis (PCA) was applied to develop a prognostic scoring system for predicting survival and immunotherapy response. The scoring system was validated using three independent cohorts of gastric cancer patients.</p>Results<p>Based on 49 CMRGs, 675 gastric cancer patients were categorized into three distinct subgroups with varying prognoses, tumor microenvironment features, and clinical characteristics. Further differential gene analysis and consensus clustering identified two additional subgroups. The prognostic scoring system developed through PCA demonstrated that the high-score subgroup had significantly improved survival, higher tumor mutational burden (TMB), and microsatellite instability (MSI), as well as a greater number of mutated genes, indicating greater sensitivity to immunotherapy. This system was validated in a real-world cohort undergoing immunotherapy. Additionally, the correlation between GPC3 expression and cholesterol levels was confirmed, highlighting GPC3’s potential biological role.</p>Conclusion<p>This study highlights the importance of CMRGs in gastric cancer, deepens our understanding of the tumor immune microenvironment, and guides individualized immunotherapy.</p>
eu_rights_str_mv openAccess
id Manara_b5d628e02afc5efcd21c2b4e4ba120ff
identifier_str_mv 10.3389/fonc.2024.1518010.s005
network_acronym_str Manara
network_name_str ManaraRepo
oai_identifier_str oai:figshare.com:article/28088252
publishDate 2024
repository.mail.fl_str_mv
repository.name.fl_str_mv
repository_id_str
rights_invalid_str_mv CC BY 4.0
spelling Image 4_Prediction of prognosis, immunogenicity and efficacy of immunotherapy based on cholesterol metabolism in gastric cancer.jpegChengjun Zhu (3948320)Mengpei Yan (20470484)Zhijun Zhang (315079)Yikai Shen (17017618)Wangwen Wang (20470487)Zetian Chen (8586345)Changsheng Cai (20470490)Hongda Liu (6702581)Zekuan Xu (308992)Zheng Li (26302)Oncology and Carcinogenesis not elsewhere classifiedgastric cancercholesterolprognosistumor microenvironmentimmunotherapyBackground<p>Cholesterol metabolism plays a crucial role in tumor progression and immune response modulation. However, the precise connection between cholesterol metabolism-related genes (CMRGs) and their implications for clinical prognosis, the tumor microenvironment (TME), and the outcomes of immunotherapy in gastric cancer remains to be fully elucidated.</p>Methods<p>Transcriptome data and related clinical information from 675 gastric cancer patients were downloaded from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases. A total of 50 cholesterol metabolism-related genes (CMRGs) were identified from the Kyoto Encyclopedia of Genes and Genomes (KEGG, hsa04979). Consensus clustering analysis was used to classify patients into distinct molecular subgroups, while principal component analysis (PCA) was applied to develop a prognostic scoring system for predicting survival and immunotherapy response. The scoring system was validated using three independent cohorts of gastric cancer patients.</p>Results<p>Based on 49 CMRGs, 675 gastric cancer patients were categorized into three distinct subgroups with varying prognoses, tumor microenvironment features, and clinical characteristics. Further differential gene analysis and consensus clustering identified two additional subgroups. The prognostic scoring system developed through PCA demonstrated that the high-score subgroup had significantly improved survival, higher tumor mutational burden (TMB), and microsatellite instability (MSI), as well as a greater number of mutated genes, indicating greater sensitivity to immunotherapy. This system was validated in a real-world cohort undergoing immunotherapy. Additionally, the correlation between GPC3 expression and cholesterol levels was confirmed, highlighting GPC3’s potential biological role.</p>Conclusion<p>This study highlights the importance of CMRGs in gastric cancer, deepens our understanding of the tumor immune microenvironment, and guides individualized immunotherapy.</p>2024-12-24T05:07:40ZImageFigureinfo:eu-repo/semantics/publishedVersionimage10.3389/fonc.2024.1518010.s005https://figshare.com/articles/figure/Image_4_Prediction_of_prognosis_immunogenicity_and_efficacy_of_immunotherapy_based_on_cholesterol_metabolism_in_gastric_cancer_jpeg/28088252CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/280882522024-12-24T05:07:40Z
spellingShingle Image 4_Prediction of prognosis, immunogenicity and efficacy of immunotherapy based on cholesterol metabolism in gastric cancer.jpeg
Chengjun Zhu (3948320)
Oncology and Carcinogenesis not elsewhere classified
gastric cancer
cholesterol
prognosis
tumor microenvironment
immunotherapy
status_str publishedVersion
title Image 4_Prediction of prognosis, immunogenicity and efficacy of immunotherapy based on cholesterol metabolism in gastric cancer.jpeg
title_full Image 4_Prediction of prognosis, immunogenicity and efficacy of immunotherapy based on cholesterol metabolism in gastric cancer.jpeg
title_fullStr Image 4_Prediction of prognosis, immunogenicity and efficacy of immunotherapy based on cholesterol metabolism in gastric cancer.jpeg
title_full_unstemmed Image 4_Prediction of prognosis, immunogenicity and efficacy of immunotherapy based on cholesterol metabolism in gastric cancer.jpeg
title_short Image 4_Prediction of prognosis, immunogenicity and efficacy of immunotherapy based on cholesterol metabolism in gastric cancer.jpeg
title_sort Image 4_Prediction of prognosis, immunogenicity and efficacy of immunotherapy based on cholesterol metabolism in gastric cancer.jpeg
topic Oncology and Carcinogenesis not elsewhere classified
gastric cancer
cholesterol
prognosis
tumor microenvironment
immunotherapy