Image 1_Peripheral immune characteristics and subset disorder in reproductive females with endometriosis.tif

<p>Pathogenesis of endometriosis (EN) is still unknown, but growing evidence suggests that immune regulation may be important, and the pattern of peripheral immune changes in reproductive women with EN has yet to be fully explored. In this study, we conducted a comprehensive and systematic ana...

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محفوظ في:
التفاصيل البيبلوغرافية
المؤلف الرئيسي: Kai-Rong Lin (3752887) (author)
مؤلفون آخرون: Pei-Xian Li (5487527) (author), Xiao-hong Zhu (20349783) (author), Xiao-fan Mao (20349786) (author), Jia-Li Peng (16652348) (author), Xiang-Ping Chen (6001580) (author), Cui-Yao SiTu (20349789) (author), Li-Fang Zhang (757908) (author), Wei Luo (80175) (author), Yu-Bin Han (20349792) (author), Si-Fei Yu (11622781) (author)
منشور في: 2024
الموضوعات:
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_version_ 1852024854056796160
author Kai-Rong Lin (3752887)
author2 Pei-Xian Li (5487527)
Xiao-hong Zhu (20349783)
Xiao-fan Mao (20349786)
Jia-Li Peng (16652348)
Xiang-Ping Chen (6001580)
Cui-Yao SiTu (20349789)
Li-Fang Zhang (757908)
Wei Luo (80175)
Yu-Bin Han (20349792)
Si-Fei Yu (11622781)
author2_role author
author
author
author
author
author
author
author
author
author
author_facet Kai-Rong Lin (3752887)
Pei-Xian Li (5487527)
Xiao-hong Zhu (20349783)
Xiao-fan Mao (20349786)
Jia-Li Peng (16652348)
Xiang-Ping Chen (6001580)
Cui-Yao SiTu (20349789)
Li-Fang Zhang (757908)
Wei Luo (80175)
Yu-Bin Han (20349792)
Si-Fei Yu (11622781)
author_role author
dc.creator.none.fl_str_mv Kai-Rong Lin (3752887)
Pei-Xian Li (5487527)
Xiao-hong Zhu (20349783)
Xiao-fan Mao (20349786)
Jia-Li Peng (16652348)
Xiang-Ping Chen (6001580)
Cui-Yao SiTu (20349789)
Li-Fang Zhang (757908)
Wei Luo (80175)
Yu-Bin Han (20349792)
Si-Fei Yu (11622781)
dc.date.none.fl_str_mv 2024-11-28T06:32:57Z
dc.identifier.none.fl_str_mv 10.3389/fimmu.2024.1431175.s005
dc.relation.none.fl_str_mv https://figshare.com/articles/figure/Image_1_Peripheral_immune_characteristics_and_subset_disorder_in_reproductive_females_with_endometriosis_tif/27923823
dc.rights.none.fl_str_mv CC BY 4.0
info:eu-repo/semantics/openAccess
dc.subject.none.fl_str_mv Genetic Immunology
endometriosis
peripheral immune profile
immune cell subset
flow cytometry
diagnosis
dc.title.none.fl_str_mv Image 1_Peripheral immune characteristics and subset disorder in reproductive females with endometriosis.tif
dc.type.none.fl_str_mv Image
Figure
info:eu-repo/semantics/publishedVersion
image
description <p>Pathogenesis of endometriosis (EN) is still unknown, but growing evidence suggests that immune regulation may be important, and the pattern of peripheral immune changes in reproductive women with EN has yet to be fully explored. In this study, we conducted a comprehensive and systematic analysis of immune cell subsets within T cells, B cells, NK cells, and γδ T cells in peripheral blood (PB) samples from women with EN, women with uterine fibroids (UF) but without EN (UF-alone), and healthy controls using multi-parameter flow cytometry. Our findings revealed that UF, a common comorbidity of EN, exhibited similar peripheral immune features to EN, particularly in T cell and B cell immunity. Compared to healthy controls, we constructed the peripheral immune profile of EN. This profile highlighted that the immunopathogenic factors in EN predominantly relate to the immune disorder of B cells and their subsets, as well as the functional abnormalities within immune cell subsets of CD4<sup>+</sup> T cells, CD8<sup>+</sup> T cells, and γδ T cells. Moreover, using the random forest (RF) machine-learning method, we developed a diagnostic model that can effectively identify the patients with EN from healthy controls. The immune factors identified within this model could be pivotal for unraveling the immune pathogenic mechanisms of EN. Our study is the first to present a comprehensive depiction of the circulating immune features in EN, although the detailed roles and underlying mechanisms of these immune factors in the context of EN require further investigation.</p>
eu_rights_str_mv openAccess
id Manara_bb8ba0141eb08ddf48bcff5d7b487a0d
identifier_str_mv 10.3389/fimmu.2024.1431175.s005
network_acronym_str Manara
network_name_str ManaraRepo
oai_identifier_str oai:figshare.com:article/27923823
publishDate 2024
repository.mail.fl_str_mv
repository.name.fl_str_mv
repository_id_str
rights_invalid_str_mv CC BY 4.0
spelling Image 1_Peripheral immune characteristics and subset disorder in reproductive females with endometriosis.tifKai-Rong Lin (3752887)Pei-Xian Li (5487527)Xiao-hong Zhu (20349783)Xiao-fan Mao (20349786)Jia-Li Peng (16652348)Xiang-Ping Chen (6001580)Cui-Yao SiTu (20349789)Li-Fang Zhang (757908)Wei Luo (80175)Yu-Bin Han (20349792)Si-Fei Yu (11622781)Genetic Immunologyendometriosisperipheral immune profileimmune cell subsetflow cytometrydiagnosis<p>Pathogenesis of endometriosis (EN) is still unknown, but growing evidence suggests that immune regulation may be important, and the pattern of peripheral immune changes in reproductive women with EN has yet to be fully explored. In this study, we conducted a comprehensive and systematic analysis of immune cell subsets within T cells, B cells, NK cells, and γδ T cells in peripheral blood (PB) samples from women with EN, women with uterine fibroids (UF) but without EN (UF-alone), and healthy controls using multi-parameter flow cytometry. Our findings revealed that UF, a common comorbidity of EN, exhibited similar peripheral immune features to EN, particularly in T cell and B cell immunity. Compared to healthy controls, we constructed the peripheral immune profile of EN. This profile highlighted that the immunopathogenic factors in EN predominantly relate to the immune disorder of B cells and their subsets, as well as the functional abnormalities within immune cell subsets of CD4<sup>+</sup> T cells, CD8<sup>+</sup> T cells, and γδ T cells. Moreover, using the random forest (RF) machine-learning method, we developed a diagnostic model that can effectively identify the patients with EN from healthy controls. The immune factors identified within this model could be pivotal for unraveling the immune pathogenic mechanisms of EN. Our study is the first to present a comprehensive depiction of the circulating immune features in EN, although the detailed roles and underlying mechanisms of these immune factors in the context of EN require further investigation.</p>2024-11-28T06:32:57ZImageFigureinfo:eu-repo/semantics/publishedVersionimage10.3389/fimmu.2024.1431175.s005https://figshare.com/articles/figure/Image_1_Peripheral_immune_characteristics_and_subset_disorder_in_reproductive_females_with_endometriosis_tif/27923823CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/279238232024-11-28T06:32:57Z
spellingShingle Image 1_Peripheral immune characteristics and subset disorder in reproductive females with endometriosis.tif
Kai-Rong Lin (3752887)
Genetic Immunology
endometriosis
peripheral immune profile
immune cell subset
flow cytometry
diagnosis
status_str publishedVersion
title Image 1_Peripheral immune characteristics and subset disorder in reproductive females with endometriosis.tif
title_full Image 1_Peripheral immune characteristics and subset disorder in reproductive females with endometriosis.tif
title_fullStr Image 1_Peripheral immune characteristics and subset disorder in reproductive females with endometriosis.tif
title_full_unstemmed Image 1_Peripheral immune characteristics and subset disorder in reproductive females with endometriosis.tif
title_short Image 1_Peripheral immune characteristics and subset disorder in reproductive females with endometriosis.tif
title_sort Image 1_Peripheral immune characteristics and subset disorder in reproductive females with endometriosis.tif
topic Genetic Immunology
endometriosis
peripheral immune profile
immune cell subset
flow cytometry
diagnosis