Inactivation of the identified genes affects mitochondrial function.
<p>(<i>A</i>) P<i>hsp-6</i>::GFP expression in animals with indicated RNAi treatments. Scale bar, 0.2 mm. (<i>B</i>) Mitochondrial morphology in a single body wall muscle cell (left), TOM20 peak number for the plot profiles of mitochondrial morphology (middl...
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2025
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| Summary: | <p>(<i>A</i>) P<i>hsp-6</i>::GFP expression in animals with indicated RNAi treatments. Scale bar, 0.2 mm. (<i>B</i>) Mitochondrial morphology in a single body wall muscle cell (left), TOM20 peak number for the plot profiles of mitochondrial morphology (middle, Mean ± s.d.), and mitochondrial lengths body wall muscles (right, Median with 95% C. I. Mann-Whitney test) in indicated animals. ****<i>P</i> < 0.0001, ***<i>P</i> < 0.001, **<i>P</i> < 0.01. Scale bar, 5 μm. (<i>C</i>) Mitochondrial morphology in animal intestine after indicated RNAi treatments. Zoomed-in fluorescence images (left bottom). Scale bar, 15 μm. (<i>D</i>) Mitochondrial membrane potential (ΔΨm) in animals with indicated RNAi treatments. ΔΨm were indicated by Tetramethylrhodamine ethyl ester (TMRE). Each data point represents the fluorescence intensity measured from a single animal. <i>n</i> = 5 per group. (<i>E</i>) ROS levels in animals with indicated RNAi treatments. ROS levels were indicated by the sensor reporter P<i>rpl-17</i>::HyPer. Each data point represents the fluorescence intensity measured from a single animal. <i>n</i> = 5 per group. (<i>F</i>) Mitochondrial distribution in early embryos with indicated RNAi treatments. Mitochondria were indicated by NAO. Scale bar, 10 μm. (<i>G</i>) Percentage of embryos with P<i>hsp-6</i>::GFP punctate patterns in wild-type (WT) and <i>drp-1 loss-of-function (lf)</i> animals after indicated RNAi treatments. <i>n</i> > 150 per group. Data represent 3 biological replicates. Mean ± s.d. Data related to Fig 5B.</p> <p>(TIF)</p> |
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