Image_2_Tpr Deficiency Disrupts Erythroid Maturation With Impaired Chromatin Condensation in Zebrafish Embryogenesis.JPEG
<p>Vertebrate erythropoiesis involves nuclear and chromatin condensation at the early stages of terminal differentiation, which is a unique process to distinguish mature erythrocytes from erythroblasts. However, the underlying mechanisms of chromatin condensation during erythrocyte maturation...
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| مؤلفون آخرون: | , , , , , , , , , , , , |
| منشور في: |
2024
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| _version_ | 1855379373980385280 |
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| author | Shuang Wu (148427) |
| author2 | Kai Chen (129938) Tao Xu (3537) Ke Ma (260231) Lei Gao (6566) Cong Fu (23214) Wenjuan Zhang (234517) Changbin Jing (763490) Chunguang Ren (10728141) Min Deng (114029) Yi Chen (38576) Yi Zhou (2723) Weijun Pan (763493) Xiaoe Jia (763491) |
| author2_role | author author author author author author author author author author author author author |
| author_facet | Shuang Wu (148427) Kai Chen (129938) Tao Xu (3537) Ke Ma (260231) Lei Gao (6566) Cong Fu (23214) Wenjuan Zhang (234517) Changbin Jing (763490) Chunguang Ren (10728141) Min Deng (114029) Yi Chen (38576) Yi Zhou (2723) Weijun Pan (763493) Xiaoe Jia (763491) |
| author_role | author |
| dc.creator.none.fl_str_mv | Shuang Wu (148427) Kai Chen (129938) Tao Xu (3537) Ke Ma (260231) Lei Gao (6566) Cong Fu (23214) Wenjuan Zhang (234517) Changbin Jing (763490) Chunguang Ren (10728141) Min Deng (114029) Yi Chen (38576) Yi Zhou (2723) Weijun Pan (763493) Xiaoe Jia (763491) |
| dc.date.none.fl_str_mv | 2024-04-24T04:30:45Z |
| dc.identifier.none.fl_str_mv | 10.3389/fcell.2021.709923.s008 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/figure/Image_2_Tpr_Deficiency_Disrupts_Erythroid_Maturation_With_Impaired_Chromatin_Condensation_in_Zebrafish_Embryogenesis_JPEG/25679394 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Cell Biology Marine Biology Cell Development, Proliferation and Death Cell Metabolism Cell Neurochemistry Cellular Interactions (incl. Adhesion, Matrix, Cell Wall) erythrocytes erythroid maturation chromatin condensation zebrafish Tpr |
| dc.title.none.fl_str_mv | Image_2_Tpr Deficiency Disrupts Erythroid Maturation With Impaired Chromatin Condensation in Zebrafish Embryogenesis.JPEG |
| dc.type.none.fl_str_mv | Image Figure info:eu-repo/semantics/publishedVersion image |
| description | <p>Vertebrate erythropoiesis involves nuclear and chromatin condensation at the early stages of terminal differentiation, which is a unique process to distinguish mature erythrocytes from erythroblasts. However, the underlying mechanisms of chromatin condensation during erythrocyte maturation remain elusive. Here, we reported a novel zebrafish mutant<sup>cas7</sup> with erythroid maturation deficiency. Positional cloning showed that a single base mutation in tprb gene, which encodes nucleoporin translocated promoter region (Tpr), is responsible for the disrupted erythroid maturation and upregulation of erythroid genes, including ae1-globin and be1-globin. Further investigation revealed that deficient erythropoiesis in tprb<sup>cas7</sup> mutant was independent on HIF signaling pathway. The proportion of euchromatin was significantly increased, whereas the percentage of heterochromatin was markedly decreased in tprb<sup>cas7</sup> mutant. In addition, TPR knockdown in human K562 cells also disrupted erythroid differentiation and dramatically elevated the expression of globin genes, which suggests that the functions of TPR in erythropoiesis are highly conserved in vertebrates. Taken together, this study revealed that Tpr played vital roles in chromatin condensation and gene regulation during erythroid maturation in vertebrates.</p> |
| eu_rights_str_mv | openAccess |
| id | Manara_bcb22dff7eecb59a88ce9bfafe7febef |
| identifier_str_mv | 10.3389/fcell.2021.709923.s008 |
| network_acronym_str | Manara |
| network_name_str | ManaraRepo |
| oai_identifier_str | oai:figshare.com:article/25679394 |
| publishDate | 2024 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Image_2_Tpr Deficiency Disrupts Erythroid Maturation With Impaired Chromatin Condensation in Zebrafish Embryogenesis.JPEGShuang Wu (148427)Kai Chen (129938)Tao Xu (3537)Ke Ma (260231)Lei Gao (6566)Cong Fu (23214)Wenjuan Zhang (234517)Changbin Jing (763490)Chunguang Ren (10728141)Min Deng (114029)Yi Chen (38576)Yi Zhou (2723)Weijun Pan (763493)Xiaoe Jia (763491)Cell BiologyMarine BiologyCell Development, Proliferation and DeathCell MetabolismCell NeurochemistryCellular Interactions (incl. Adhesion, Matrix, Cell Wall)erythrocyteserythroid maturationchromatin condensationzebrafishTpr<p>Vertebrate erythropoiesis involves nuclear and chromatin condensation at the early stages of terminal differentiation, which is a unique process to distinguish mature erythrocytes from erythroblasts. However, the underlying mechanisms of chromatin condensation during erythrocyte maturation remain elusive. Here, we reported a novel zebrafish mutant<sup>cas7</sup> with erythroid maturation deficiency. Positional cloning showed that a single base mutation in tprb gene, which encodes nucleoporin translocated promoter region (Tpr), is responsible for the disrupted erythroid maturation and upregulation of erythroid genes, including ae1-globin and be1-globin. Further investigation revealed that deficient erythropoiesis in tprb<sup>cas7</sup> mutant was independent on HIF signaling pathway. The proportion of euchromatin was significantly increased, whereas the percentage of heterochromatin was markedly decreased in tprb<sup>cas7</sup> mutant. In addition, TPR knockdown in human K562 cells also disrupted erythroid differentiation and dramatically elevated the expression of globin genes, which suggests that the functions of TPR in erythropoiesis are highly conserved in vertebrates. Taken together, this study revealed that Tpr played vital roles in chromatin condensation and gene regulation during erythroid maturation in vertebrates.</p>2024-04-24T04:30:45ZImageFigureinfo:eu-repo/semantics/publishedVersionimage10.3389/fcell.2021.709923.s008https://figshare.com/articles/figure/Image_2_Tpr_Deficiency_Disrupts_Erythroid_Maturation_With_Impaired_Chromatin_Condensation_in_Zebrafish_Embryogenesis_JPEG/25679394CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/256793942024-04-24T04:30:45Z |
| spellingShingle | Image_2_Tpr Deficiency Disrupts Erythroid Maturation With Impaired Chromatin Condensation in Zebrafish Embryogenesis.JPEG Shuang Wu (148427) Cell Biology Marine Biology Cell Development, Proliferation and Death Cell Metabolism Cell Neurochemistry Cellular Interactions (incl. Adhesion, Matrix, Cell Wall) erythrocytes erythroid maturation chromatin condensation zebrafish Tpr |
| status_str | publishedVersion |
| title | Image_2_Tpr Deficiency Disrupts Erythroid Maturation With Impaired Chromatin Condensation in Zebrafish Embryogenesis.JPEG |
| title_full | Image_2_Tpr Deficiency Disrupts Erythroid Maturation With Impaired Chromatin Condensation in Zebrafish Embryogenesis.JPEG |
| title_fullStr | Image_2_Tpr Deficiency Disrupts Erythroid Maturation With Impaired Chromatin Condensation in Zebrafish Embryogenesis.JPEG |
| title_full_unstemmed | Image_2_Tpr Deficiency Disrupts Erythroid Maturation With Impaired Chromatin Condensation in Zebrafish Embryogenesis.JPEG |
| title_short | Image_2_Tpr Deficiency Disrupts Erythroid Maturation With Impaired Chromatin Condensation in Zebrafish Embryogenesis.JPEG |
| title_sort | Image_2_Tpr Deficiency Disrupts Erythroid Maturation With Impaired Chromatin Condensation in Zebrafish Embryogenesis.JPEG |
| topic | Cell Biology Marine Biology Cell Development, Proliferation and Death Cell Metabolism Cell Neurochemistry Cellular Interactions (incl. Adhesion, Matrix, Cell Wall) erythrocytes erythroid maturation chromatin condensation zebrafish Tpr |